FastQCFastQC Report
Thu 2 Feb 2017
SRR4062745_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062745_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2735
Sequences flagged as poor quality0
Sequence length38
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT1224.460694698354662No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT812.9616087751371114No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT772.8153564899451555No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT461.6819012797074957No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT250.9140767824497258No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.6946983546617916No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.6581352833638026No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.6581352833638026No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT160.5850091407678245No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT150.5484460694698354No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA150.5484460694698354No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA140.5118829981718465No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA130.47531992687385743No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA120.43875685557586835No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA110.40219378427787933No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC110.40219378427787933No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC100.3656307129798903No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.3656307129798903No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT90.3290676416819013No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTT90.3290676416819013No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA80.29250457038391225No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG80.29250457038391225No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.29250457038391225No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA80.29250457038391225No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA70.25594149908592323No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC70.25594149908592323No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA70.25594149908592323No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA60.21937842778793418No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTAT60.21937842778793418No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG60.21937842778793418No Hit
TATCAACGCAGAGTACATGGGCGCAGAGTACTTTTTTT60.21937842778793418No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA60.21937842778793418No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA60.21937842778793418No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC50.18281535648994515No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCA50.18281535648994515No Hit
GTATCAACGCAGAGTACATGGGAACGCAGAGTACTTTT50.18281535648994515No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT50.18281535648994515No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA50.18281535648994515No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG50.18281535648994515No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCT40.14625228519195613No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA40.14625228519195613No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA40.14625228519195613No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG40.14625228519195613No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT40.14625228519195613No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG40.14625228519195613No Hit
GTATCAACGCAGAGTACATGGGTGTGGTATCAACGCAA40.14625228519195613No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA40.14625228519195613No Hit
ACGCAGAGTACATGGGACGCAGAGTACTTTTTTTTTTT40.14625228519195613No Hit
TATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.14625228519195613No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA30.10968921389396709No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCA30.10968921389396709No Hit
TATCAACGCAGAGTACATGGGGTATCAACGCAAAAAAA30.10968921389396709No Hit
GGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA30.10968921389396709No Hit
TCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAA30.10968921389396709No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG30.10968921389396709No Hit
ATACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA30.10968921389396709No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT30.10968921389396709No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTA30.10968921389396709No Hit
CAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAA30.10968921389396709No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC30.10968921389396709No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA30.10968921389396709No Hit
CTGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT30.10968921389396709No Hit
GTACATGGGAGAGTACTTTTTTTCTTTTTTTTTTTTTT30.10968921389396709No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG30.10968921389396709No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT30.10968921389396709No Hit
CAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAA30.10968921389396709No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA30.10968921389396709No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT30.10968921389396709No Hit
GGTATCAACGCAGAGTACATGGGCAGTGGTATCAACGC30.10968921389396709No Hit
GCAGAGAAACATGAAGGCTGCATTCTTTTTGAACCCAA30.10968921389396709No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAG30.10968921389396709No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.10968921389396709No Hit
GGCTCAAAGCATAGACAAGCAAAAAGGACTTGAAAACA30.10968921389396709No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA30.10968921389396709No Hit
GTATCAACGCAGAGTACATGGGACGCAGAGTACTTTTT30.10968921389396709No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG30.10968921389396709No Hit
GTGGGATGGCAGCATTGGCACTGATTCAGCCCTCGCCC30.10968921389396709No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)