FastQCFastQC Report
Thu 2 Feb 2017
SRR4062744_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062744_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3245593
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT40630.12518513565933867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10900.023.0673541
GTATCAA34800.021.7213781
TAGGACC10800.016.2936444
GTAGGAC24450.016.0967793
TAGGACG29800.015.5679374
TTAGGAC18700.015.22864153
TGTAGGA28400.015.21559052
TATCAAC49700.014.9418712
GTCCTAC23100.014.9056571
GTCCTAA16550.014.5150281
CTGTAGG29900.014.4080681
AGGACGT31900.014.3926425
GACGTGA16550.014.3071517
ATCAACG51800.014.2999323
CGCGTAA2350.014.29774910
TAGTTCG901.5376722E-414.2199085
GGACGTG30950.014.1107886
TCAACGC52550.014.0338714
CGTGATT12100.014.01597528
CACGTTT14500.014.01323928
Produced by FastQC (version 0.11.5)