##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062744_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3245593
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.353875239440065	32.0	32.0	32.0	32.0	32.0
2	31.05636350583699	32.0	32.0	32.0	32.0	32.0
3	31.087044185762046	32.0	32.0	32.0	32.0	32.0
4	31.149667256492112	32.0	32.0	32.0	32.0	32.0
5	31.101355592028945	32.0	32.0	32.0	32.0	32.0
6	34.75331965529874	36.0	36.0	36.0	32.0	36.0
7	34.713957665055354	36.0	36.0	36.0	32.0	36.0
8	34.714269780591714	36.0	36.0	36.0	32.0	36.0
9	34.82129613910308	36.0	36.0	36.0	32.0	36.0
10	34.60610988500407	36.0	36.0	36.0	32.0	36.0
11	34.78526389476438	36.0	36.0	36.0	32.0	36.0
12	34.65433219753678	36.0	36.0	36.0	32.0	36.0
13	34.70432367829238	36.0	36.0	36.0	32.0	36.0
14	34.637426812295935	36.0	36.0	36.0	32.0	36.0
15	34.605047213251936	36.0	36.0	36.0	32.0	36.0
16	34.6125456888772	36.0	36.0	36.0	32.0	36.0
17	34.57134674618783	36.0	36.0	36.0	32.0	36.0
18	34.58660158559622	36.0	36.0	36.0	32.0	36.0
19	34.56162217505399	36.0	36.0	36.0	32.0	36.0
20	34.55097727903653	36.0	36.0	36.0	32.0	36.0
21	34.542445710229224	36.0	36.0	36.0	32.0	36.0
22	34.518505246961034	36.0	36.0	36.0	32.0	36.0
23	34.47329162960359	36.0	36.0	36.0	32.0	36.0
24	34.45870692967356	36.0	36.0	36.0	32.0	36.0
25	34.432997606292595	36.0	36.0	36.0	32.0	36.0
26	34.406970313283274	36.0	36.0	36.0	32.0	36.0
27	34.40106908044231	36.0	36.0	36.0	32.0	36.0
28	34.37713786047727	36.0	36.0	36.0	32.0	36.0
29	34.34808061269543	36.0	36.0	36.0	32.0	36.0
30	34.325310043495904	36.0	36.0	36.0	32.0	36.0
31	34.333230629965	36.0	36.0	36.0	32.0	36.0
32	34.30573395986496	36.0	36.0	36.0	32.0	36.0
33	34.28678457218758	36.0	36.0	36.0	32.0	36.0
34	34.28359285960994	36.0	36.0	36.0	32.0	36.0
35	34.26921983132204	36.0	36.0	36.0	32.0	36.0
36	34.22704695259079	36.0	36.0	36.0	32.0	36.0
37	34.20877972068587	36.0	36.0	36.0	32.0	36.0
38	33.70104168945397	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	4.0
7	4.0
8	5.0
9	11.0
10	31.0
11	25.0
12	12.0
13	17.0
14	146.0
15	501.0
16	716.0
17	900.0
18	1059.0
19	1335.0
20	1761.0
21	2690.0
22	3986.0
23	6226.0
24	9821.0
25	15393.0
26	23680.0
27	35642.0
28	50963.0
29	70557.0
30	96891.0
31	132802.0
32	184136.0
33	282573.0
34	673427.0
35	1650279.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.0305271263114	17.812415935952142	12.03035190290155	26.126705034834906
2	15.679874961272214	19.770577363859307	39.324217467503345	25.225330207365136
3	18.478224696139204	23.827296381526644	29.259412874749323	28.43506604758483
4	12.583061008052365	16.259282836020024	36.667780001022955	34.48987615490466
5	14.025180060044665	36.976242180143856	34.6562590892733	14.342318670538184
6	33.31505208307657	36.142463112955944	17.53256116618766	13.009923637779828
7	29.715460055028515	30.9191241037839	21.342775889745777	18.022639951441803
8	27.07644062167047	34.937536549730126	19.644821353854383	18.341201474745024
9	27.379224392520918	14.780078167726312	18.951406295013072	38.8892911447397
10	15.611937980770119	27.57724827719226	33.07855559602092	23.7322581460167
11	36.060886353566545	21.450909899855862	23.666578134426448	18.82162561215114
12	24.776013631139637	23.70277706739465	29.95033138089237	21.570877920573345
13	29.551872518603155	20.78432170119822	25.182857607726984	24.480948172471642
14	22.68586865453277	20.468777973240595	25.869560461333336	30.975792910893297
15	24.051822330482462	29.301546817485892	22.38644796564162	24.260182886390027
16	24.19536146104178	26.62032400625407	25.40436071718446	23.779953815519686
17	23.08987811830691	27.010000154085578	26.369897841261036	23.530223886346477
18	23.51498968396195	25.94674607263378	27.71393762063109	22.824326622773178
19	24.967646116301427	25.428920058175365	26.046139719476425	23.557294106046786
20	25.45106753780414	25.548250884633443	25.910916703333815	23.089764874228603
21	25.406412566181952	25.17297511654155	25.25848051817198	24.162131799104518
22	25.29277764305639	26.098815516575712	25.412147294878473	23.19625954548942
23	23.78271879446807	26.278631352482634	26.173679770104886	23.764970082944412
24	24.652859202632357	25.46841397000388	26.372562403066972	23.50616442429679
25	24.237737421249918	25.8197034522458	26.1761511902957	23.76640793620858
26	23.630646932730485	26.41656363750664	26.450584831724516	23.502204598038357
27	24.075656974386597	26.227948327012463	25.865643330173928	23.83075136842702
28	23.608868631941174	26.184624713750104	25.91479797967348	24.29170867463524
29	24.09831382860221	25.87837540108237	25.69891482432492	24.324395945990503
30	23.789029473461067	25.945402490097045	26.164228826657006	24.101339209784882
31	24.750826316537083	25.47837310776055	25.696803533094414	24.073997042607953
32	23.89916293676515	26.228433281136283	25.798993897197075	24.073409884901494
33	23.355422496065117	26.425185466386797	26.568961203353002	23.65043083419508
34	24.13549774745626	25.920153333210077	26.459407990829593	23.484940928504077
35	23.809349187003768	26.175042641278605	26.537651440096514	23.47795673162111
36	23.55488871385835	26.080012375398592	26.531332315197954	23.833766595545104
37	23.959542773188584	26.0028644465973	25.555602803312112	24.48198997690201
38	23.705355258337345	26.611351911474205	25.829852281379306	23.85344054880914
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	30.0
1	96.0
2	162.0
3	162.0
4	379.5
5	597.0
6	597.0
7	726.0
8	855.0
9	960.0
10	1065.0
11	1065.0
12	1522.5
13	1980.0
14	3109.5
15	4239.0
16	4239.0
17	6831.5
18	9424.0
19	9424.0
20	13030.5
21	16637.0
22	20921.5
23	25206.0
24	25206.0
25	33159.5
26	41113.0
27	41113.0
28	53928.0
29	66743.0
30	87114.0
31	107485.0
32	107485.0
33	132964.5
34	158444.0
35	158444.0
36	176073.0
37	193702.0
38	211876.5
39	230051.0
40	230051.0
41	235524.0
42	240997.0
43	253617.5
44	266238.0
45	266238.0
46	272267.0
47	278296.0
48	278296.0
49	283113.5
50	287931.0
51	282400.5
52	276870.0
53	276870.0
54	267904.5
55	258939.0
56	258939.0
57	241731.0
58	224523.0
59	201103.0
60	177683.0
61	177683.0
62	159387.5
63	141092.0
64	117176.5
65	93261.0
66	93261.0
67	76253.5
68	59246.0
69	59246.0
70	46621.5
71	33997.0
72	26633.0
73	19269.0
74	19269.0
75	15341.0
76	11413.0
77	11413.0
78	9485.0
79	7557.0
80	5832.5
81	4108.0
82	4108.0
83	3420.0
84	2732.0
85	2732.0
86	1966.0
87	1200.0
88	905.5
89	611.0
90	611.0
91	453.5
92	296.0
93	253.0
94	210.0
95	210.0
96	202.5
97	195.0
98	195.0
99	695.5
100	1196.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12376782917636316
2	0.05539819687804355
3	0.010999530748310093
4	0.0026805579134537203
5	2.7729909449521244E-4
6	2.7729909449521244E-4
7	9.243303149840415E-5
8	4.6216515749202073E-4
9	2.464880839957444E-4
10	5.854091994898929E-4
11	0.0021875817454622315
12	0.002557313871455848
13	0.01371089967226328
14	0.009366547191838286
15	0.02270771473810795
16	0.010691420643315413
17	0.019811479751157955
18	0.004960572690414356
19	0.007178965446376056
20	0.0055767929004037166
21	0.005669225931902121
22	0.006531934225887226
23	0.00884276001334733
24	0.013556844619765942
25	0.01592929242822498
26	0.017161732848203704
27	0.00804167374036116
28	0.005453548858405845
29	0.009613035275834032
30	0.0029886680184484007
31	0.005114627742911696
32	0.006131391089394141
33	0.00804167374036116
34	0.011492506916301583
35	0.015343883228735087
36	0.01371089967226328
37	0.008904382034346266
38	0.005114627742911696
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	3245593.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.161662644822318
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.30886466815793	16.983689800339345
2	13.913259667057224	8.392940887351857
3	7.691409322964487	6.959570797874883
4	5.04602606668056	6.087861436807949
5	3.521957815340394	5.311405173779618
6	2.481999150153077	4.491673263099169
7	1.854137901449757	3.9146717316354467
8	1.5007202041175423	3.6211373216689755
9	1.1672333137531294	3.168512769349794
>10	6.291960194908085	32.032049340428586
>50	0.154302256132158	3.0799905848483773
>100	0.05981543930531397	3.5197822503328156
>500	0.005953234554588983	1.22016839519635
>1k	0.002360765425959728	1.216546247286916
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4063	0.12518513565933867	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.464880839957444E-4
2	0.0	0.0	0.0	0.0	2.7729909449521244E-4
3	0.0	0.0	0.0	0.0	3.081101049946805E-4
4	0.0	0.0	0.0	0.0	3.081101049946805E-4
5	0.0	0.0	0.0	0.0	3.3892111549414854E-4
6	0.0	0.0	0.0	0.0	4.0054313649308463E-4
7	0.0	0.0	0.0	0.0	4.0054313649308463E-4
8	0.0	0.0	0.0	0.0	4.0054313649308463E-4
9	0.0	0.0	0.0	0.0	4.0054313649308463E-4
10	0.0	0.0	0.0	0.0	4.0054313649308463E-4
11	0.0	0.0	0.0	0.0	4.0054313649308463E-4
12	0.0	0.0	0.0	0.0	0.0010475743569819135
13	0.0	0.0	0.0	0.0	0.0011708183989797857
14	0.0	0.0	0.0	0.0	0.0011708183989797857
15	0.0	0.0	0.0	0.0	0.0014173064829755301
16	0.0	0.0	0.0	0.0	0.0014789285039744663
17	0.0	0.0	0.0	0.0	0.0015097395144739343
18	0.0	0.0	0.0	3.081101049946805E-5	0.0015097395144739343
19	0.0	0.0	0.0	3.081101049946805E-5	0.0015097395144739343
20	0.0	0.0	0.0	6.16220209989361E-5	0.0016946055774707427
21	0.0	0.0	0.0	6.16220209989361E-5	0.001848660629968083
22	0.0	0.0	0.0	1.232440419978722E-4	0.002002715682465423
23	0.0	0.0	0.0	1.848660629968083E-4	0.002002715682465423
24	0.0	0.0	0.0	4.313541469925527E-4	0.002002715682465423
25	0.0	0.0	0.0	4.6216515749202073E-4	0.002002715682465423
26	0.0	0.0	0.0	7.702752624867012E-4	0.0022800147769606355
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1090	0.0	23.067354	1
GTATCAA	3480	0.0	21.721378	1
TAGGACC	1080	0.0	16.293644	4
GTAGGAC	2445	0.0	16.096779	3
TAGGACG	2980	0.0	15.567937	4
TTAGGAC	1870	0.0	15.2286415	3
TGTAGGA	2840	0.0	15.2155905	2
TATCAAC	4970	0.0	14.941871	2
GTCCTAC	2310	0.0	14.905657	1
GTCCTAA	1655	0.0	14.515028	1
CTGTAGG	2990	0.0	14.408068	1
AGGACGT	3190	0.0	14.392642	5
GACGTGA	1655	0.0	14.307151	7
ATCAACG	5180	0.0	14.299932	3
CGCGTAA	235	0.0	14.297749	10
TAGTTCG	90	1.5376722E-4	14.219908	5
GGACGTG	3095	0.0	14.110788	6
TCAACGC	5255	0.0	14.033871	4
CGTGATT	1210	0.0	14.015975	28
CACGTTT	1450	0.0	14.013239	28
>>END_MODULE