FastQCFastQC Report
Thu 2 Feb 2017
SRR4062744_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062744_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3245593
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT40280.12410675029185729No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10250.022.9462091
GTATCAA32300.021.3497221
GTAGGAC26800.015.34437753
GCTCGTA2950.015.18565126
TAGGACC12800.015.1260784
CGCGTAA1603.0559022E-1015.00060710
TAGGACG31750.014.9679974
TTAGGAC20550.014.9499713
TGTAGGA30250.014.9153862
GTCCTAC22550.014.9001361
AGGACGT32700.014.6323645
TAGCGGC2100.014.47543830
GACGTGA17750.014.4248977
GGACGTG32250.014.3403326
CTAATAC3850.014.1308773
ATTTAGA12800.014.1249171
CACGTTT12500.014.079267528
TATCAAC48550.014.0388372
GGTCGCG2300.013.9153217
CTAGCGG2300.013.9123229
Produced by FastQC (version 0.11.5)