##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062744_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3245593
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29367453035547	32.0	32.0	32.0	32.0	32.0
2	31.38306312590642	32.0	32.0	32.0	32.0	32.0
3	31.424987051672836	32.0	32.0	32.0	32.0	32.0
4	31.502014577921507	32.0	32.0	32.0	32.0	32.0
5	31.451157923991087	32.0	32.0	32.0	32.0	32.0
6	34.978697575450774	36.0	36.0	36.0	36.0	36.0
7	35.00422973552137	36.0	36.0	36.0	36.0	36.0
8	34.94655522118762	36.0	36.0	36.0	36.0	36.0
9	35.03752318913678	36.0	36.0	36.0	36.0	36.0
10	34.91384132267971	36.0	36.0	36.0	32.0	36.0
11	35.06063576055285	36.0	36.0	36.0	36.0	36.0
12	34.98368495372032	36.0	36.0	36.0	36.0	36.0
13	35.022278209251745	36.0	36.0	36.0	36.0	36.0
14	34.97694288840283	36.0	36.0	36.0	36.0	36.0
15	34.950640453069745	36.0	36.0	36.0	36.0	36.0
16	34.969254925063	36.0	36.0	36.0	36.0	36.0
17	34.950987076937864	36.0	36.0	36.0	36.0	36.0
18	34.9451902934225	36.0	36.0	36.0	36.0	36.0
19	34.93481160453575	36.0	36.0	36.0	36.0	36.0
20	34.92894333947602	36.0	36.0	36.0	36.0	36.0
21	34.915921990218735	36.0	36.0	36.0	32.0	36.0
22	34.902147003644636	36.0	36.0	36.0	32.0	36.0
23	34.858607656597734	36.0	36.0	36.0	32.0	36.0
24	34.838492996503255	36.0	36.0	36.0	32.0	36.0
25	34.82780619751152	36.0	36.0	36.0	32.0	36.0
26	34.76715472334332	36.0	36.0	36.0	32.0	36.0
27	34.744500003543266	36.0	36.0	36.0	32.0	36.0
28	34.71976862163555	36.0	36.0	36.0	32.0	36.0
29	34.696851083915945	36.0	36.0	36.0	32.0	36.0
30	34.6749247364041	36.0	36.0	36.0	32.0	36.0
31	34.66551228080662	36.0	36.0	36.0	32.0	36.0
32	34.64791950192153	36.0	36.0	36.0	32.0	36.0
33	34.63376399936776	36.0	36.0	36.0	32.0	36.0
34	34.62530514454524	36.0	36.0	36.0	32.0	36.0
35	34.61242090428467	36.0	36.0	36.0	32.0	36.0
36	34.58546774040984	36.0	36.0	36.0	32.0	36.0
37	34.568841811034225	36.0	36.0	36.0	32.0	36.0
38	34.19542746117582	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	3.0
20	11.0
21	55.0
22	171.0
23	665.0
24	1830.0
25	4596.0
26	9924.0
27	19505.0
28	34341.0
29	54881.0
30	83438.0
31	120691.0
32	171754.0
33	270926.0
34	684427.0
35	1788373.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.56125001810312	18.929823680236353	12.616256693917382	26.89266960774314
2	14.836579940861347	20.68407837951339	38.530863235162265	25.948478444462996
3	17.51692217724157	24.638147789941623	29.473473722675642	28.37145631014117
4	12.090447737188988	17.145126598054528	36.55301799027418	34.2114076744823
5	13.540391540159224	37.53930329526838	34.31403752719457	14.606267637377822
6	33.38585588444628	36.50803089730989	17.227900012016306	12.878213206227526
7	29.582166745697297	31.085320114112218	21.31291292734697	18.01960021284351
8	27.044824516726578	35.14003752245786	19.444138895333666	18.370999065481897
9	27.231777925744566	14.657216374932098	18.7764310471848	39.334574652138535
10	15.380507993083464	27.844597350962363	33.01363464380217	23.761260012152004
11	36.14508802088356	21.430426788742178	23.580131157017128	18.84435403335713
12	24.715767639968647	23.824587501047578	29.792727595403477	21.6669172635803
13	29.57581184056633	20.800452799307863	25.16129588844918	24.46243947167663
14	22.64541487487803	20.45629257889082	25.794515824997156	31.103776721233995
15	23.98883655467583	29.413761984327667	22.317185180027195	24.280216280969302
16	24.220820115405754	26.689614714357194	25.20230515727177	23.887260012965278
17	23.05880620274939	27.1068183841905	26.214161787999913	23.620213625060195
18	23.57824902875992	26.055793194032645	27.56645703882156	22.799500738385866
19	24.973926182364824	25.544515285804476	25.967488837941172	23.51406969388953
20	25.502889610619693	25.642648354245278	25.716964511569994	23.13749752356503
21	25.371219443508984	25.286643063334925	25.197013668097632	24.145123825058455
22	25.27307028330416	26.109188675228225	25.382634236640268	23.235106804827346
23	23.77552158531618	26.293464415979116	26.104623325340942	23.82639067336376
24	24.774209212307273	25.51616915614496	26.11510438924412	23.594517242303642
25	24.252885993882774	25.902725206426325	25.99722269209071	23.84716610760019
26	23.626267142101394	26.496930759297765	26.277895150682877	23.59890694791796
27	24.107026490415144	26.274737805680264	25.72103245358547	23.897203250319127
28	23.672499909877796	26.21825965239634	25.871728217308824	24.23751222041704
29	24.103884867880847	25.884299109592607	25.621542812053143	24.390273210473403
30	23.817773824382787	25.97066237202262	26.091965320359023	24.119598483235574
31	24.706887154365937	25.580903089204345	25.6182768449402	24.093932911489517
32	23.949182784163018	26.258067477961657	25.734495976544192	24.058253761331134
33	23.34263723147049	26.509701000710812	26.412276585511492	23.73538518230721
34	24.137129948209772	25.97574618875503	26.352133493016535	23.53499037001867
35	23.87378824147082	26.136949395688248	26.302620199143885	23.68664216369705
36	23.60385297848498	26.12533364472995	26.398534874828727	23.872278501956345
37	24.052522913378233	26.04454717520034	25.405988982598863	24.49694092882256
38	23.836367552165385	26.492309104874888	25.784795434791384	23.886527908168343
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	48.0
2	89.0
3	89.0
4	276.0
5	463.0
6	463.0
7	586.0
8	709.0
9	814.0
10	919.0
11	919.0
12	1384.5
13	1850.0
14	3012.5
15	4175.0
16	4175.0
17	6718.5
18	9262.0
19	9262.0
20	12778.5
21	16295.0
22	20615.0
23	24935.0
24	24935.0
25	32901.0
26	40867.0
27	40867.0
28	53509.5
29	66152.0
30	86633.0
31	107114.0
32	107114.0
33	132689.5
34	158265.0
35	158265.0
36	176272.0
37	194279.0
38	213442.0
39	232605.0
40	232605.0
41	238076.5
42	243548.0
43	255660.5
44	267773.0
45	267773.0
46	273167.5
47	278562.0
48	278562.0
49	283230.0
50	287898.0
51	282423.0
52	276948.0
53	276948.0
54	268154.5
55	259361.0
56	259361.0
57	242073.0
58	224785.0
59	201588.5
60	178392.0
61	178392.0
62	159143.5
63	139895.0
64	116136.0
65	92377.0
66	92377.0
67	75778.5
68	59180.0
69	59180.0
70	46565.5
71	33951.0
72	26459.0
73	18967.0
74	18967.0
75	15015.5
76	11064.0
77	11064.0
78	9209.5
79	7355.0
80	5628.5
81	3902.0
82	3902.0
83	3104.0
84	2306.0
85	2306.0
86	1601.5
87	897.0
88	600.5
89	304.0
90	304.0
91	195.0
92	86.0
93	59.5
94	33.0
95	33.0
96	24.5
97	16.0
98	16.0
99	11.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009120059107842542
2	0.0
3	0.0
4	1.848660629968083E-4
5	0.0
6	9.243303149840415E-5
7	4.929761679914888E-4
8	0.0021567707349627635
9	0.003697321259936166
10	0.0011400073884803177
11	0.005237871784909569
12	2.7729909449521244E-4
13	1.5405505249734024E-4
14	0.0
15	0.0
16	3.081101049946805E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.848660629968083E-4
22	0.0
23	2.1567707349627631E-4
24	0.0
25	1.232440419978722E-4
26	6.16220209989361E-5
27	1.232440419978722E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	6.16220209989361E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	3245593.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.038418739252887
#Duplication Level	Percentage of deduplicated	Percentage of total
1	51.75972345351395	14.512608000175526
2	15.296772167452627	8.577946067799715
3	8.554870862931777	7.195951545453445
4	5.524784165729685	6.196248475308912
5	3.8265281750465645	5.364489964475236
6	2.7795014340634596	4.67596950567751
7	2.1318329903366333	4.184125824564852
8	1.616788897075228	3.6265763287336057
9	1.3050651137170963	3.2932765926351566
>10	6.958696481109142	33.02543775459979
>50	0.1700808841760819	3.1821274070926315
>100	0.0664142998643386	3.6817974091379537
>500	0.006512634864589043	1.2997380142314328
>1k	0.002428440118804794	1.183707110114236
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4028	0.12410675029185729	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.232440419978722E-4
2	0.0	0.0	0.0	0.0	1.5405505249734024E-4
3	0.0	0.0	0.0	0.0	1.5405505249734024E-4
4	0.0	0.0	0.0	0.0	1.5405505249734024E-4
5	0.0	0.0	0.0	0.0	1.5405505249734024E-4
6	0.0	0.0	0.0	0.0	2.1567707349627634E-4
7	0.0	0.0	0.0	0.0	2.1567707349627634E-4
8	0.0	0.0	0.0	0.0	2.1567707349627634E-4
9	0.0	0.0	0.0	0.0	2.1567707349627634E-4
10	0.0	0.0	0.0	0.0	3.081101049946805E-4
11	0.0	0.0	0.0	0.0	3.081101049946805E-4
12	0.0	0.0	0.0	0.0	8.318972834856373E-4
13	0.0	0.0	0.0	0.0	0.0011091963779808498
14	0.0	0.0	0.0	0.0	0.0011091963779808498
15	0.0	0.0	0.0	0.0	0.0016021725459723385
16	0.0	0.0	0.0	0.0	0.0016329835564718065
17	0.0	0.0	0.0	0.0	0.0016329835564718065
18	0.0	0.0	0.0	3.081101049946805E-5	0.0017870386089691467
19	0.0	0.0	0.0	6.16220209989361E-5	0.001817849619468615
20	0.0	0.0	0.0	9.243303149840415E-5	0.002002715682465423
21	0.0	0.0	0.0	1.232440419978722E-4	0.002033526692964891
22	0.0	0.0	0.0	1.848660629968083E-4	0.002095148713963827
23	0.0	0.0	0.0	1.848660629968083E-4	0.002125959724463295
24	0.0	0.0	0.0	3.697321259936166E-4	0.002125959724463295
25	0.0	0.0	0.0	4.313541469925527E-4	0.002125959724463295
26	0.0	0.0	0.0	5.854091994898929E-4	0.0022183927559616995
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1025	0.0	22.946209	1
GTATCAA	3230	0.0	21.349722	1
GTAGGAC	2680	0.0	15.3443775	3
GCTCGTA	295	0.0	15.185651	26
TAGGACC	1280	0.0	15.126078	4
CGCGTAA	160	3.0559022E-10	15.000607	10
TAGGACG	3175	0.0	14.967997	4
TTAGGAC	2055	0.0	14.949971	3
TGTAGGA	3025	0.0	14.915386	2
GTCCTAC	2255	0.0	14.900136	1
AGGACGT	3270	0.0	14.632364	5
TAGCGGC	210	0.0	14.475438	30
GACGTGA	1775	0.0	14.424897	7
GGACGTG	3225	0.0	14.340332	6
CTAATAC	385	0.0	14.130877	3
ATTTAGA	1280	0.0	14.124917	1
CACGTTT	1250	0.0	14.0792675	28
TATCAAC	4855	0.0	14.038837	2
GGTCGCG	230	0.0	13.915321	7
CTAGCGG	230	0.0	13.91232	29
>>END_MODULE