FastQCFastQC Report
Thu 2 Feb 2017
SRR4062743_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062743_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences825786
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT28710.34766876648429496No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT19690.23843949885321378No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA11280.1365971329133698No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT10880.1317532629519028No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC8670.10499088141479754No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCT555.8294667E-520.36421415
ACCGAGT555.8349047E-520.3617488
GTCTATA507.1512745E-419.216841
AAATCGC601.13744114E-418.66719614
TTAGGAC1006.823939E-817.5962353
GTCCTAA1201.6370905E-917.3485371
CATCGTT2050.017.1691428
GTAGTAC753.237846E-517.0630173
CGTCGTA1456.91216E-1116.55219510
ATCGTTT2150.016.37156529
CGCGGTT2250.016.3560210
GTATTAA1001.3013141E-616.0140341
CAAATCG703.6940232E-416.00045413
CAACTAA805.6164183E-516.00045426
GTGCGCC600.00244066116.00045411
GCATCGT2250.015.64299427
GTACAGA1451.204171E-915.4618251
CCTAGAA1357.876224E-915.41064552
CGTATAT1357.894414E-915.40784510
GACCGCG2700.015.4059787
Produced by FastQC (version 0.11.5)