##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062743_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	825786
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20649780935981	32.0	32.0	32.0	32.0	32.0
2	30.838740303177822	32.0	32.0	32.0	32.0	32.0
3	30.906438229759285	32.0	32.0	32.0	32.0	32.0
4	30.973511297115717	32.0	32.0	32.0	32.0	32.0
5	30.8900187215574	32.0	32.0	32.0	32.0	32.0
6	34.536674150445755	36.0	36.0	36.0	32.0	36.0
7	34.45675513995151	36.0	36.0	36.0	32.0	36.0
8	34.43568914948909	36.0	36.0	36.0	32.0	36.0
9	34.56291218305953	36.0	36.0	36.0	32.0	36.0
10	34.30418776777519	36.0	36.0	36.0	32.0	36.0
11	34.57250183461575	36.0	36.0	36.0	32.0	36.0
12	34.40754989791544	36.0	36.0	36.0	32.0	36.0
13	34.48136442129075	36.0	36.0	36.0	32.0	36.0
14	34.38911897271206	36.0	36.0	36.0	32.0	36.0
15	34.344031020143234	36.0	36.0	36.0	32.0	36.0
16	34.35473718372557	36.0	36.0	36.0	32.0	36.0
17	34.287303248057	36.0	36.0	36.0	32.0	36.0
18	34.31840573707958	36.0	36.0	36.0	32.0	36.0
19	34.30167258829769	36.0	36.0	36.0	32.0	36.0
20	34.26032047043665	36.0	36.0	36.0	32.0	36.0
21	34.23527645176838	36.0	36.0	36.0	32.0	36.0
22	34.21052790916775	36.0	36.0	36.0	32.0	36.0
23	34.166659400861725	36.0	36.0	36.0	32.0	36.0
24	34.158296459373254	36.0	36.0	36.0	32.0	36.0
25	34.13658260130349	36.0	36.0	36.0	32.0	36.0
26	34.10261738513368	36.0	36.0	36.0	32.0	36.0
27	34.098776196254235	36.0	36.0	36.0	32.0	36.0
28	34.08435115150899	36.0	36.0	36.0	32.0	36.0
29	34.05702930299133	36.0	36.0	36.0	32.0	36.0
30	34.0074668255456	36.0	36.0	36.0	32.0	36.0
31	34.03675165236514	36.0	36.0	36.0	32.0	36.0
32	33.98807681408985	36.0	36.0	36.0	32.0	36.0
33	33.926706192645554	36.0	36.0	36.0	32.0	36.0
34	33.918407432434066	36.0	36.0	36.0	32.0	36.0
35	33.882298803806314	36.0	36.0	36.0	32.0	36.0
36	33.8392585972637	36.0	36.0	36.0	32.0	36.0
37	33.8374227705483	36.0	36.0	36.0	32.0	36.0
38	33.233056748358536	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	10.0
11	8.0
12	0.0
13	7.0
14	140.0
15	254.0
16	315.0
17	375.0
18	456.0
19	658.0
20	850.0
21	1213.0
22	1733.0
23	2485.0
24	3525.0
25	5436.0
26	7631.0
27	11088.0
28	15697.0
29	21416.0
30	29055.0
31	39350.0
32	53656.0
33	80129.0
34	177163.0
35	373130.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.41250812310259	17.49207088195071	11.703693465630788	25.391727529315915
2	16.128156756121466	20.28441853167146	38.37299611187242	25.214428600334653
3	18.813947291929075	24.91764363918225	28.576324968510804	27.692084100377873
4	12.36996795671371	15.998028478077112	36.50343078722822	35.12857277798096
5	14.227778141068026	36.99687328193988	33.776789628305636	14.998558948686464
6	33.05012805996718	36.05018255357024	17.579757442917952	13.319931943544628
7	29.378797896791664	30.97545853284023	21.46221902526805	18.183524545100063
8	27.82789183114692	33.10070563332013	19.906924700556928	19.16447783497602
9	27.791286825610573	14.487686852736308	18.70452806060665	39.01649826104647
10	15.811717406573178	27.04352248782969	31.989634042965438	25.155126062631695
11	36.62643336437518	21.496378518395627	22.770224530648477	19.10696358658072
12	24.684139841915517	24.11956970600452	29.222135702703323	21.974154749376634
13	29.74044015323369	19.951384759231022	25.236202972869208	25.07197211466608
14	23.749321797430582	20.11306605693026	25.724586780863063	30.413025364776097
15	25.042969114321462	27.248898670410238	23.16189696375817	24.546235251510126
16	25.162771770724333	25.989104949036935	24.561824206487618	24.286299073751113
17	23.67866767600303	26.05868281604845	25.780590461771386	24.482059046177138
18	24.42206454566262	25.10872053101396	26.988607808017285	23.48060711530613
19	25.17457533928048	25.362169651139975	26.015661521245754	23.44759348833379
20	25.420686832184252	24.584823423552166	25.794536490608948	24.199953253654634
21	26.63484851053729	24.597630714007174	24.881620473421222	23.88590030203431
22	25.398586222389618	24.745225745430414	25.370005486048118	24.48618254613185
23	24.00285814632256	24.618691792517954	25.917937290331956	25.46051277082753
24	24.27129318436431	25.57737608623248	25.83389348109123	24.317437248311986
25	24.705929374603343	24.751711374768064	25.74910494978514	24.793254300843454
26	24.101783352470097	25.63732686074735	26.255032484048662	24.005857302733894
27	24.84159125700002	25.289810490824888	25.278910806673515	24.58968744550158
28	24.01759893040357	25.167608860986373	26.14225923180887	24.67253297680119
29	24.03824610484374	25.481739846555367	26.09018959555773	24.389824453043158
30	24.222475725451158	25.533991634393445	26.163834131384107	24.07969850877129
31	24.548555899624457	25.4213498454112	25.242358662967355	24.787735591996988
32	24.114578855010734	25.68965663401301	25.092250922509223	25.10351358846703
33	23.90860724878317	25.123317964760066	25.815933177588768	25.152141608867996
34	24.517728503674565	25.410209331505335	25.99942350268144	24.072638662138658
35	25.129837412553535	25.123781563087416	25.905955080130997	23.84042594422805
36	24.11587596103072	25.914160033717828	25.525872133863647	24.44409187138781
37	24.951556489977037	25.723382713417887	25.247546336217336	24.077514460387743
38	24.153457318941413	25.793438862791117	25.674878684720753	24.378225133546717
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	788.0
1	535.5
2	283.0
3	283.0
4	621.0
5	959.0
6	959.0
7	1048.0
8	1137.0
9	1107.5
10	1078.0
11	1078.0
12	1327.5
13	1577.0
14	1962.0
15	2347.0
16	2347.0
17	3584.0
18	4821.0
19	4821.0
20	5655.5
21	6490.0
22	6898.0
23	7306.0
24	7306.0
25	8657.0
26	10008.0
27	10008.0
28	12584.0
29	15160.0
30	18417.0
31	21674.0
32	21674.0
33	26320.5
34	30967.0
35	30967.0
36	34227.5
37	37488.0
38	41983.0
39	46478.0
40	46478.0
41	49888.0
42	53298.0
43	59582.0
44	65866.0
45	65866.0
46	68723.0
47	71580.0
48	71580.0
49	74184.0
50	76788.0
51	75561.5
52	74335.0
53	74335.0
54	70851.5
55	67368.0
56	67368.0
57	64916.5
58	62465.0
59	56021.0
60	49577.0
61	49577.0
62	46181.5
63	42786.0
64	35139.5
65	27493.0
66	27493.0
67	22677.0
68	17861.0
69	17861.0
70	14443.5
71	11026.0
72	8580.5
73	6135.0
74	6135.0
75	4585.0
76	3035.0
77	3035.0
78	2887.0
79	2739.0
80	2179.0
81	1619.0
82	1619.0
83	1577.5
84	1536.0
85	1536.0
86	1031.5
87	527.0
88	467.5
89	408.0
90	408.0
91	291.0
92	174.0
93	150.5
94	127.0
95	127.0
96	113.0
97	99.0
98	99.0
99	241.0
100	383.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1184326205578685
2	0.05485682731361394
3	0.012836255397887587
4	0.00339070897302691
5	0.0
6	1.2109674903667536E-4
7	0.0
8	3.6329024711002607E-4
9	2.4219349807335071E-4
10	7.265804942200521E-4
11	0.0023008382316968322
12	0.003027418725916884
13	0.014410513135364367
14	0.008961159428713977
15	0.023371672564078346
16	0.010898707413300783
17	0.019496576594904735
18	0.0054493537066503915
19	0.008234578934493924
20	0.0064181276989437935
21	0.006297030949907119
22	0.006660321197017145
23	0.009203352926787329
24	0.013441739143070966
25	0.016226964370914497
26	0.018164512355501303
27	0.008961159428713977
28	0.00653922444798047
29	0.009808836671970704
30	0.0029063219768802086
31	0.005691547204723742
32	0.005933740702797093
33	0.009082256177750651
34	0.01332064239403429
35	0.0167113513670612
36	0.01332064239403429
37	0.00992993342100738
38	0.0054493537066503915
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	825786.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.46847300569608
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.14872346002052	52.77322510380401
2	11.170361757481928	14.179316073372034
3	2.765862314641214	5.266351729628339
4	1.0562093274906759	2.681439727608255
5	0.5088206519308741	1.6147034905907682
6	0.2887757490684226	1.0996893500669311
7	0.18249808820232416	0.8108012489262248
8	0.1170470007123688	0.5943035524088536
9	0.0995137999319488	0.5684390032207619
>10	0.5235555729159849	6.400770032732674
>50	0.06960490379917532	3.185113079086636
>100	0.06308334996164613	7.814719355493629
>500	0.005177053024490738	2.1524401007107894
>1k	7.669708184430722E-4	0.8586881523500499
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2871	0.34766876648429496	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1969	0.23843949885321378	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1128	0.1365971329133698	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1088	0.1317532629519028	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	867	0.10499088141479754	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2109674903667536E-4
2	0.0	0.0	0.0	0.0	1.2109674903667536E-4
3	0.0	0.0	0.0	0.0	1.2109674903667536E-4
4	0.0	0.0	0.0	0.0	2.4219349807335071E-4
5	0.0	0.0	0.0	0.0	2.4219349807335071E-4
6	0.0	0.0	0.0	0.0	2.4219349807335071E-4
7	0.0	0.0	0.0	0.0	2.4219349807335071E-4
8	0.0	0.0	0.0	0.0	2.4219349807335071E-4
9	0.0	0.0	0.0	0.0	2.4219349807335071E-4
10	0.0	0.0	0.0	0.0	2.4219349807335071E-4
11	0.0	0.0	0.0	0.0	2.4219349807335071E-4
12	0.0	0.0	0.0	0.0	3.6329024711002607E-4
13	0.0	0.0	0.0	0.0	3.6329024711002607E-4
14	0.0	0.0	0.0	0.0	4.8438699614670143E-4
15	0.0	0.0	0.0	0.0	4.8438699614670143E-4
16	0.0	0.0	0.0	0.0	7.265804942200521E-4
17	0.0	0.0	0.0	0.0	7.265804942200521E-4
18	0.0	0.0	0.0	0.0	7.265804942200521E-4
19	0.0	0.0	0.0	0.0	7.265804942200521E-4
20	0.0	0.0	0.0	0.0	7.265804942200521E-4
21	0.0	0.0	0.0	1.2109674903667536E-4	9.687739922934029E-4
22	0.0	0.0	0.0	8.476772432567275E-4	9.687739922934029E-4
23	0.0	0.0	0.0	0.002058644733623481	9.687739922934029E-4
24	0.0	0.0	0.0	0.0038750959691736114	9.687739922934029E-4
25	0.0	0.0	0.0	0.004722773212430339	9.687739922934029E-4
26	0.0	0.0	0.0	0.006297030949907119	9.687739922934029E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCGCT	55	5.8294667E-5	20.364214	15
ACCGAGT	55	5.8349047E-5	20.361748	8
GTCTATA	50	7.1512745E-4	19.21684	1
AAATCGC	60	1.13744114E-4	18.667196	14
TTAGGAC	100	6.823939E-8	17.596235	3
GTCCTAA	120	1.6370905E-9	17.348537	1
CATCGTT	205	0.0	17.16914	28
GTAGTAC	75	3.237846E-5	17.063017	3
CGTCGTA	145	6.91216E-11	16.552195	10
ATCGTTT	215	0.0	16.371565	29
CGCGGTT	225	0.0	16.35602	10
GTATTAA	100	1.3013141E-6	16.014034	1
CAAATCG	70	3.6940232E-4	16.000454	13
CAACTAA	80	5.6164183E-5	16.000454	26
GTGCGCC	60	0.002440661	16.000454	11
GCATCGT	225	0.0	15.642994	27
GTACAGA	145	1.204171E-9	15.461825	1
CCTAGAA	135	7.876224E-9	15.4106455	2
CGTATAT	135	7.894414E-9	15.407845	10
GACCGCG	270	0.0	15.405978	7
>>END_MODULE