FastQCFastQC Report
Thu 2 Feb 2017
SRR4062743_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062743_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences825786
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT32650.39538088560474505No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24530.29705032538696463No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15490.18757886425781015No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11190.13550726217203973No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA11090.13429629468167298No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC10990.13308532719130622No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG9040.10947146112915453No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTAG400.004479730520.002081
ATGCGTG659.42993E-619.69197313
TAACGAA751.5077621E-619.19967313
ATAACGA802.7947026E-617.99969312
ACGAACG802.7947026E-617.99969315
GTATAGA1006.7992005E-817.6018311
AGGACGT1850.017.298055
TAGGACG1850.017.2970034
ATTATAC652.0997747E-417.2304763
CGAACGA854.9813134E-616.94088716
CTAGGAC1354.4201443E-1016.592313
GTAGGAC2250.016.3552783
GTATTAC600.002439429516.0016651
CCGATAA908.5714E-616.0006969
GGACGTG1800.016.0006966
CGATAAC908.5714E-616.00069610
GGCGGGT1304.794856E-915.99972732
GTAATAC600.002441402315.9997263
ACCGTCG1850.015.5682458
TACCGTC1652.910383E-1115.5158267
Produced by FastQC (version 0.11.5)