##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062743_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	825786
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.12781519667323	32.0	32.0	32.0	32.0	32.0
2	31.231108301666534	32.0	32.0	32.0	32.0	32.0
3	31.340043304197454	32.0	32.0	32.0	32.0	32.0
4	31.45146200105112	32.0	32.0	32.0	32.0	32.0
5	31.360621274761257	32.0	32.0	32.0	32.0	32.0
6	34.8761531437927	36.0	36.0	36.0	36.0	36.0
7	34.91102779654777	36.0	36.0	36.0	36.0	36.0
8	34.839145977287096	36.0	36.0	36.0	32.0	36.0
9	34.96959745018685	36.0	36.0	36.0	36.0	36.0
10	34.80189661728341	36.0	36.0	36.0	32.0	36.0
11	34.99177026493547	36.0	36.0	36.0	36.0	36.0
12	34.891083161981435	36.0	36.0	36.0	32.0	36.0
13	34.93945162548166	36.0	36.0	36.0	36.0	36.0
14	34.88179988520028	36.0	36.0	36.0	32.0	36.0
15	34.84552172112387	36.0	36.0	36.0	32.0	36.0
16	34.86519509897237	36.0	36.0	36.0	32.0	36.0
17	34.831590751114696	36.0	36.0	36.0	32.0	36.0
18	34.83510013490178	36.0	36.0	36.0	32.0	36.0
19	34.821843673784734	36.0	36.0	36.0	32.0	36.0
20	34.80474239088577	36.0	36.0	36.0	32.0	36.0
21	34.79550634183675	36.0	36.0	36.0	32.0	36.0
22	34.77375857667725	36.0	36.0	36.0	32.0	36.0
23	34.71463551089507	36.0	36.0	36.0	32.0	36.0
24	34.695664494190986	36.0	36.0	36.0	32.0	36.0
25	34.67798557979913	36.0	36.0	36.0	32.0	36.0
26	34.60608559602609	36.0	36.0	36.0	32.0	36.0
27	34.594479683598415	36.0	36.0	36.0	32.0	36.0
28	34.55500335437995	36.0	36.0	36.0	32.0	36.0
29	34.52689800989603	36.0	36.0	36.0	32.0	36.0
30	34.49613459177075	36.0	36.0	36.0	32.0	36.0
31	34.49086930512264	36.0	36.0	36.0	32.0	36.0
32	34.45403772890313	36.0	36.0	36.0	32.0	36.0
33	34.40900184793639	36.0	36.0	36.0	32.0	36.0
34	34.39530459465285	36.0	36.0	36.0	32.0	36.0
35	34.34876832496555	36.0	36.0	36.0	32.0	36.0
36	34.32241767237517	36.0	36.0	36.0	32.0	36.0
37	34.30010317443018	36.0	36.0	36.0	32.0	36.0
38	33.865893827214315	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	2.0
21	14.0
22	54.0
23	167.0
24	565.0
25	1291.0
26	2800.0
27	5694.0
28	9916.0
29	15735.0
30	24383.0
31	35191.0
32	50039.0
33	79948.0
34	191660.0
35	408323.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.709496462472664	18.734846521451633	12.440687983064436	26.11496903301127
2	15.317061947116986	21.250083254115783	37.44098040046743	25.991874398299796
3	17.726021027239504	25.688979953644164	29.02180468063154	27.563194338484788
4	11.961951355377005	16.98492949133249	36.436481650792885	34.616637502497625
5	13.832518352212317	37.516620528805284	33.34265778300916	15.308203335973241
6	33.122220822878624	36.50833632039371	17.114402797826067	13.255040058901601
7	29.12165893260223	31.099173633783167	21.52427269116379	18.254894742450812
8	27.667932973342165	33.43146503649361	19.683540394860014	19.217061595304212
9	27.551580194077406	14.197708031336248	18.573108423463193	39.67760335112316
10	15.50440917534737	27.267134750501953	32.03015822654515	25.198297847605534
11	36.77816261777315	21.320996923971226	22.657495095308448	19.243345362947174
12	24.593749205299698	24.22004328013534	29.179215362873784	22.006992151691183
13	29.79843421713884	20.003996197557473	25.22981163377877	24.967757951524913
14	23.63554238022926	19.960256046966162	25.804990639221298	30.599210933583276
15	25.00078712886874	27.371740378257805	23.001237608775156	24.626234884098302
16	25.03666208516745	25.956514104760924	24.530234867429172	24.476588942642454
17	23.491921635871762	26.041250396591852	25.87571114065872	24.591116826877666
18	24.485520461717687	24.870487026905273	27.15521939098023	23.48877312039681
19	25.231113145536494	25.23232411302686	26.194558880872282	23.342003860564358
20	25.408883173122334	24.42606195794068	25.9935382774714	24.171516591465586
21	26.552310831511832	24.550700242799277	24.952378645773415	23.944610279915473
22	25.30186428670901	24.67240262295876	25.602305684893768	24.42342740543846
23	23.86041904319037	24.535412322320795	26.118025735481105	25.486142899007735
24	24.300242435691572	25.384179436318853	26.014851305301857	24.300726822687718
25	24.712908839247113	24.59798760691368	25.86333213447116	24.825771419368042
26	24.096344690203868	25.592981223926326	26.40057642122344	23.91009766464637
27	24.998062447322784	25.173653158695252	25.406401674045515	24.42188271993645
28	24.025837202374465	24.942902882829205	26.363973232774597	24.667286682021736
29	24.140152533465088	25.25133630262562	26.26249415708186	24.346017006827434
30	24.162434335287834	25.2265114690731	26.410837674651788	24.20021652098728
31	24.53347477433621	24.998486290637043	25.535429275865663	24.93260965916109
32	24.315258432572122	25.26865313773786	25.481662319293374	24.93442611039664
33	23.948214186241955	24.795164849004465	26.052391297503224	25.204229667250356
34	24.4835829137331	25.10953200950367	26.292041763846807	24.11484331291642
35	25.280036232147314	24.850506063314224	26.049000588530202	23.820457116008264
36	24.223346000053283	25.567762107858456	25.792396577321487	24.416495314766777
37	25.06957008232157	25.407793302381005	25.398226659207108	24.124409956090318
38	24.26624363484442	25.305739387370807	25.876469056715727	24.551547921069044
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	393.0
1	282.0
2	171.0
3	171.0
4	512.0
5	853.0
6	853.0
7	995.5
8	1138.0
9	1130.0
10	1122.0
11	1122.0
12	1366.5
13	1611.0
14	2134.0
15	2657.0
16	2657.0
17	3957.5
18	5258.0
19	5258.0
20	6056.5
21	6855.0
22	7272.0
23	7689.0
24	7689.0
25	8709.0
26	9729.0
27	9729.0
28	12296.0
29	14863.0
30	18211.5
31	21560.0
32	21560.0
33	26507.5
34	31455.0
35	31455.0
36	34417.5
37	37380.0
38	42101.5
39	46823.0
40	46823.0
41	49974.5
42	53126.0
43	59093.5
44	65061.0
45	65061.0
46	68615.5
47	72170.0
48	72170.0
49	74701.5
50	77233.0
51	75810.0
52	74387.0
53	74387.0
54	71184.5
55	67982.0
56	67982.0
57	65206.0
58	62430.0
59	55977.5
60	49525.0
61	49525.0
62	46092.5
63	42660.0
64	35203.0
65	27746.0
66	27746.0
67	22808.5
68	17871.0
69	17871.0
70	14298.5
71	10726.0
72	8370.0
73	6014.0
74	6014.0
75	4447.0
76	2880.0
77	2880.0
78	2841.5
79	2803.0
80	2182.5
81	1562.0
82	1562.0
83	1469.0
84	1376.0
85	1376.0
86	867.0
87	358.0
88	302.5
89	247.0
90	247.0
91	144.5
92	42.0
93	30.5
94	19.0
95	19.0
96	12.5
97	6.0
98	6.0
99	5.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008234578934493924
2	1.2109674903667536E-4
3	0.0
4	1.2109674903667536E-4
5	0.0
6	2.4219349807335071E-4
7	2.4219349807335071E-4
8	0.003027418725916884
9	0.003996192718210287
10	9.687739922934029E-4
11	0.005570450455687066
12	3.6329024711002607E-4
13	1.2109674903667536E-4
14	0.0
15	0.0
16	1.2109674903667536E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	1.2109674903667536E-4
22	1.2109674903667536E-4
23	0.0
24	0.0
25	3.6329024711002607E-4
26	1.2109674903667536E-4
27	2.4219349807335071E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.2109674903667536E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	825786.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.856697209139696
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.9217232178189	50.674072279355734
2	12.097006806574658	14.96561774342381
3	2.941853184407134	5.459199649848466
4	1.085214757928304	2.6851120275224374
5	0.5281360528814075	1.633437595415771
6	0.29854039884738126	1.1080033833718956
7	0.19474711616725496	0.843248937797773
8	0.13187396074848054	0.6525830127830986
9	0.09596125238991547	0.5342261519603552
>10	0.555883422311879	6.5109682833033755
>50	0.07088826124503382	3.0812340961598164
>100	0.07206749154226037	8.577477141175848
>500	0.004922642852755856	1.9844771576444358
>1k	0.0011814342846614054	1.2903425402372277
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3265	0.39538088560474505	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2453	0.29705032538696463	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1549	0.18757886425781015	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1119	0.13550726217203973	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1109	0.13429629468167298	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1099	0.13308532719130622	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	904	0.10947146112915453	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	1.2109674903667536E-4
5	0.0	0.0	0.0	0.0	1.2109674903667536E-4
6	0.0	0.0	0.0	0.0	1.2109674903667536E-4
7	0.0	0.0	0.0	1.2109674903667536E-4	1.2109674903667536E-4
8	0.0	0.0	0.0	1.2109674903667536E-4	1.2109674903667536E-4
9	0.0	0.0	0.0	1.2109674903667536E-4	1.2109674903667536E-4
10	0.0	0.0	0.0	1.2109674903667536E-4	1.2109674903667536E-4
11	0.0	0.0	0.0	1.2109674903667536E-4	1.2109674903667536E-4
12	0.0	0.0	0.0	1.2109674903667536E-4	1.2109674903667536E-4
13	0.0	0.0	0.0	1.2109674903667536E-4	1.2109674903667536E-4
14	0.0	0.0	0.0	1.2109674903667536E-4	2.4219349807335071E-4
15	0.0	0.0	0.0	1.2109674903667536E-4	3.6329024711002607E-4
16	0.0	0.0	0.0	1.2109674903667536E-4	3.6329024711002607E-4
17	0.0	0.0	0.0	1.2109674903667536E-4	3.6329024711002607E-4
18	0.0	0.0	0.0	1.2109674903667536E-4	3.6329024711002607E-4
19	0.0	0.0	0.0	1.2109674903667536E-4	3.6329024711002607E-4
20	0.0	0.0	0.0	1.2109674903667536E-4	4.8438699614670143E-4
21	0.0	0.0	0.0	2.4219349807335071E-4	4.8438699614670143E-4
22	0.0	0.0	0.0	7.265804942200521E-4	4.8438699614670143E-4
23	0.0	0.0	0.0	0.0019375479845868057	7.265804942200521E-4
24	0.0	0.0	0.0	0.0038750959691736114	7.265804942200521E-4
25	0.0	0.0	0.0	0.004480579714356989	7.265804942200521E-4
26	0.0	0.0	0.0	0.006175934200870444	7.265804942200521E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATTAG	40	0.0044797305	20.00208	1
ATGCGTG	65	9.42993E-6	19.691973	13
TAACGAA	75	1.5077621E-6	19.199673	13
ATAACGA	80	2.7947026E-6	17.999693	12
ACGAACG	80	2.7947026E-6	17.999693	15
GTATAGA	100	6.7992005E-8	17.601831	1
AGGACGT	185	0.0	17.29805	5
TAGGACG	185	0.0	17.297003	4
ATTATAC	65	2.0997747E-4	17.230476	3
CGAACGA	85	4.9813134E-6	16.940887	16
CTAGGAC	135	4.4201443E-10	16.59231	3
GTAGGAC	225	0.0	16.355278	3
GTATTAC	60	0.0024394295	16.001665	1
CCGATAA	90	8.5714E-6	16.000696	9
GGACGTG	180	0.0	16.000696	6
CGATAAC	90	8.5714E-6	16.000696	10
GGCGGGT	130	4.794856E-9	15.999727	32
GTAATAC	60	0.0024414023	15.999726	3
ACCGTCG	185	0.0	15.568245	8
TACCGTC	165	2.910383E-11	15.515826	7
>>END_MODULE