FastQCFastQC Report
Thu 2 Feb 2017
SRR4062742_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062742_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1944962
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT55370.2846842251930886No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT38870.19984966287259084No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG24210.12447543962298493No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA23610.1213905464476941No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG23270.11964244031502928No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA22210.11419246237201548No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG22080.11352406885070249No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA21230.10915380351904048No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC20980.10786843136266928No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA20590.10586325079873025No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT20290.10432080421108483No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT19630.10092742171826494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG4350.015.44780429
TCTAGCG4450.015.10027328
CGTTTTT3700.014.70225430
TACCGTC4500.014.5769597
TCTTATA1654.820322E-1014.5513712
TCCTACA7600.014.5322242
GTCCTAA4850.014.5303381
ACCGTCG4100.014.4382118
GTATATG2003.6379788E-1214.4147561
TTAGGAC5900.014.3720733
AGGCCCG7200.014.22251910
CTATTCC7250.014.1215294
GTCCTAC6850.014.0289591
CGTCGTA4000.014.00029310
AACCGCG3550.013.97104557
CGTTTAT4500.013.86624331
ACGTTTT3700.013.83741629
CCTATTC7400.013.8370593
GTATCAA57200.013.7483411
AACGAAT2107.2759576E-1213.71386731
Produced by FastQC (version 0.11.5)