##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062742_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1944962
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23512078899228	32.0	32.0	32.0	32.0	32.0
2	30.865790694111247	32.0	32.0	32.0	32.0	32.0
3	30.918805611626347	32.0	32.0	32.0	32.0	32.0
4	30.98808871330134	32.0	32.0	32.0	32.0	32.0
5	30.900059744097828	32.0	32.0	32.0	32.0	32.0
6	34.55571728393665	36.0	36.0	36.0	32.0	36.0
7	34.480051538281984	36.0	36.0	36.0	32.0	36.0
8	34.46835619410559	36.0	36.0	36.0	32.0	36.0
9	34.58826907672232	36.0	36.0	36.0	32.0	36.0
10	34.32355285090403	36.0	36.0	36.0	32.0	36.0
11	34.587678833828114	36.0	36.0	36.0	32.0	36.0
12	34.413122724248595	36.0	36.0	36.0	32.0	36.0
13	34.49245795033528	36.0	36.0	36.0	32.0	36.0
14	34.39998159347072	36.0	36.0	36.0	32.0	36.0
15	34.34110126573167	36.0	36.0	36.0	32.0	36.0
16	34.36468527405677	36.0	36.0	36.0	32.0	36.0
17	34.28782567474326	36.0	36.0	36.0	32.0	36.0
18	34.31089759080126	36.0	36.0	36.0	32.0	36.0
19	34.29815286879641	36.0	36.0	36.0	32.0	36.0
20	34.27264285883221	36.0	36.0	36.0	32.0	36.0
21	34.254334017836854	36.0	36.0	36.0	32.0	36.0
22	34.231815325954955	36.0	36.0	36.0	32.0	36.0
23	34.17070410630131	36.0	36.0	36.0	32.0	36.0
24	34.15418450334762	36.0	36.0	36.0	32.0	36.0
25	34.13272392982485	36.0	36.0	36.0	32.0	36.0
26	34.086910181278604	36.0	36.0	36.0	32.0	36.0
27	34.08053782027618	36.0	36.0	36.0	32.0	36.0
28	34.06324596573095	36.0	36.0	36.0	32.0	36.0
29	34.02836405030021	36.0	36.0	36.0	32.0	36.0
30	33.9962837320215	36.0	36.0	36.0	32.0	36.0
31	34.02135003151732	36.0	36.0	36.0	32.0	36.0
32	33.97151666716368	36.0	36.0	36.0	32.0	36.0
33	33.92337022522805	36.0	36.0	36.0	32.0	36.0
34	33.92769164641777	36.0	36.0	36.0	32.0	36.0
35	33.887598832265105	36.0	36.0	36.0	32.0	36.0
36	33.83307951517819	36.0	36.0	36.0	32.0	36.0
37	33.83446771710707	36.0	36.0	36.0	32.0	36.0
38	33.22104853462433	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	3.0
8	5.0
9	11.0
10	9.0
11	13.0
12	7.0
13	8.0
14	210.0
15	522.0
16	750.0
17	814.0
18	1131.0
19	1440.0
20	1855.0
21	2594.0
22	3813.0
23	5623.0
24	8255.0
25	12207.0
26	17823.0
27	26115.0
28	36762.0
29	50672.0
30	68190.0
31	92963.0
32	127840.0
33	191119.0
34	425433.0
35	868772.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.21526556291493	17.47332376906355	11.651936764630939	25.659473903390577
2	16.549943876348948	19.797605047939165	38.063770048386715	25.588681027325173
3	18.58415452617636	24.87628115339286	28.507527001149775	28.03203731928101
4	12.497133543454453	15.705816721596372	36.391606809569595	35.40544292537958
5	14.641293025350677	36.71081843412557	33.23958666459635	15.4083018759274
6	33.246047860134844	35.8629667771917	17.40746205961154	13.483523303061915
7	29.931808401299563	30.601744713647218	21.185309114167328	18.281137770885895
8	28.264150070310112	32.49987788920566	19.662305811702584	19.57366622878164
9	28.31534492296998	14.03538177727414	18.456304409971228	39.19296888978465
10	16.39378904342014	26.090542173320653	31.06809943700352	26.44756934625569
11	37.23909006213096	21.00369988750203	22.683148544621794	19.074061505745213
12	25.38618475580537	23.39381898727148	28.66759832260798	22.55239793431517
13	29.748179260617576	19.418494690892114	25.21339019281865	25.61993585567166
14	24.11238990518824	19.893860605789488	24.920544569855732	31.073204919166535
15	25.544332961342047	26.968819587655375	22.4245946237831	25.062252827219478
16	25.743010566358677	25.30352121113332	24.036524871744902	24.916943350763106
17	24.190984569734464	25.486036651058985	25.301007171306544	25.021971607900007
18	25.03091483752807	24.258159588947066	26.710059700986033	24.00086587253883
19	25.986021263762904	24.47435766251836	25.42087637307687	24.118744700641862
20	26.189637592892435	23.82961832155269	25.06595600601998	24.914788079534897
21	27.496133373919196	23.640305052117185	24.14162957722146	24.72193199674216
22	26.14031759057728	24.0942272104877	24.713047046247205	25.052408152687818
23	24.391347540194612	23.936850700436242	25.48941067098316	26.18239108838598
24	25.0941668247898	24.825588899396152	25.15931869755904	24.92092557825501
25	25.15395062369608	24.23973461097995	25.240600077033164	25.3657146882908
26	24.756273031799957	25.007533706264653	25.786513791567806	24.449679470367595
27	25.548472883455393	24.54971156550592	24.802435611928985	25.0993799391097
28	24.620177534994877	24.504950495049506	25.410004648159042	25.464867321796575
29	24.805437816975026	24.502519859868947	25.376616338029905	25.315425985126115
30	24.514784246864117	24.785285473657364	25.919066608806173	24.78086367067234
31	25.36991189574544	24.557820505898896	24.58286093307727	25.489406665278384
32	24.77037945043266	24.88313942696542	24.525063514254906	25.821417608347012
33	24.42024886877828	24.454905388728918	25.364716166186756	25.760129576306046
34	25.237154475390856	24.55320137973306	25.53205493427345	24.677589210602633
35	25.9524163362317	24.32848124015573	25.36254428154242	24.356558142070153
36	24.754264345999914	25.08249467390628	24.95933759489394	25.20390338519986
37	25.754701700710882	24.942474065123214	24.644650409430398	24.65817382473551
38	24.84066804501555	24.945561046441377	25.04819164979723	25.16557925874584
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	607.0
1	515.0
2	423.0
3	423.0
4	1048.0
5	1673.0
6	1673.0
7	1806.5
8	1940.0
9	1837.5
10	1735.0
11	1735.0
12	2222.5
13	2710.0
14	3523.5
15	4337.0
16	4337.0
17	6550.5
18	8764.0
19	8764.0
20	10363.5
21	11963.0
22	12477.5
23	12992.0
24	12992.0
25	15320.0
26	17648.0
27	17648.0
28	23319.0
29	28990.0
30	36223.5
31	43457.0
32	43457.0
33	56060.5
34	68664.0
35	68664.0
36	75292.5
37	81921.0
38	93410.0
39	104899.0
40	104899.0
41	110800.5
42	116702.0
43	132676.5
44	148651.0
45	148651.0
46	154757.0
47	160863.0
48	160863.0
49	171430.5
50	181998.0
51	183034.0
52	184070.0
53	184070.0
54	176775.5
55	169481.0
56	169481.0
57	164320.5
58	159160.0
59	142678.0
60	126196.0
61	126196.0
62	119247.0
63	112298.0
64	92326.0
65	72354.0
66	72354.0
67	59782.0
68	47210.0
69	47210.0
70	38064.0
71	28918.0
72	22366.0
73	15814.0
74	15814.0
75	11936.0
76	8058.0
77	8058.0
78	7826.5
79	7595.0
80	5919.5
81	4244.0
82	4244.0
83	4242.5
84	4241.0
85	4241.0
86	2814.0
87	1387.0
88	1197.5
89	1008.0
90	1008.0
91	734.0
92	460.0
93	410.0
94	360.0
95	360.0
96	338.0
97	316.0
98	316.0
99	585.5
100	855.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11902546167997113
2	0.05347148170504102
3	0.011825423838614842
4	0.0026735740852520513
5	2.0565954501938855E-4
6	1.542446587645414E-4
7	5.141488625484714E-5
8	3.5990420378392997E-4
9	3.084893175290828E-4
10	6.169786350581656E-4
11	0.0019537656776841914
12	0.0019537656776841914
13	0.014653242582631435
14	0.00827779668703039
15	0.024267826312287848
16	0.010540051682243663
17	0.020925858705722784
18	0.005295733284249256
19	0.007763647824481918
20	0.005398563056758949
21	0.005861297033052573
22	0.006735350099384975
23	0.008946190208343403
24	0.014139093720082964
25	0.017738135757922262
26	0.018920678141783748
27	0.007866477596991613
28	0.005449977943013796
29	0.010848540999772746
30	0.0027764038577617455
31	0.005501392829268643
32	0.006324031009346197
33	0.008329211573285237
34	0.012236742928653618
35	0.015578710535218683
36	0.014961731900160516
37	0.009614583729656415
38	0.006324031009346197
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1944962.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.0698882181159
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.45719980576062	37.02676359720711
2	13.916033526291411	13.657164191493454
3	4.58769690171711	6.753533224375658
4	2.0660203687973127	4.055175542129389
5	1.1103731726264567	2.7242943730587466
6	0.666471337323001	1.9622204413810727
7	0.4293882581829284	1.4749023681845452
8	0.31682353326681123	1.243719628981676
9	0.21039611578511272	0.9291702494791125
>10	1.0545379204526224	9.118460709025976
>50	0.08230316672768655	2.840586005446954
>100	0.08315450143710323	8.804285519149072
>500	0.013594513551076352	4.54287509457957
>1k	0.00590149425473082	4.580521230821505
>5k	1.0538382597733608E-4	0.28632782468625023
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5537	0.2846842251930886	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3887	0.19984966287259084	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2421	0.12447543962298493	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2361	0.1213905464476941	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2327	0.11964244031502928	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2221	0.11419246237201548	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2208	0.11352406885070249	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2123	0.10915380351904048	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2098	0.10786843136266928	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2059	0.10586325079873025	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2029	0.10432080421108483	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1963	0.10092742171826494	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.570744312742357E-4
2	0.0	0.0	0.0	0.0	2.570744312742357E-4
3	0.0	0.0	0.0	5.141488625484714E-5	3.0848931752908284E-4
4	0.0	0.0	0.0	5.141488625484714E-5	3.0848931752908284E-4
5	0.0	0.0	0.0	5.141488625484714E-5	3.0848931752908284E-4
6	0.0	0.0	0.0	5.141488625484714E-5	3.0848931752908284E-4
7	0.0	0.0	0.0	5.141488625484714E-5	3.5990420378392997E-4
8	0.0	0.0	0.0	5.141488625484714E-5	3.5990420378392997E-4
9	0.0	0.0	0.0	5.141488625484714E-5	3.5990420378392997E-4
10	0.0	0.0	0.0	1.0282977250969428E-4	4.113190900387771E-4
11	0.0	0.0	0.0	1.0282977250969428E-4	4.113190900387771E-4
12	0.0	0.0	0.0	1.0282977250969428E-4	6.683935213130128E-4
13	0.0	0.0	0.0	1.0282977250969428E-4	7.198084075678599E-4
14	0.0	0.0	0.0	1.0282977250969428E-4	7.198084075678599E-4
15	0.0	0.0	0.0	1.0282977250969428E-4	9.254679525872485E-4
16	0.0	0.0	0.0	1.5424465876454142E-4	9.254679525872485E-4
17	0.0	0.0	0.0	2.0565954501938855E-4	9.254679525872485E-4
18	0.0	0.0	0.0	2.570744312742357E-4	0.00107971261135179
19	0.0	0.0	0.0	3.5990420378392997E-4	0.00107971261135179
20	0.0	0.0	0.0	3.5990420378392997E-4	0.0012853721563711785
21	0.0	0.0	0.0	5.655637488033186E-4	0.0015424465876454142
22	0.0	0.0	0.0	8.740530663324013E-4	0.0015424465876454142
23	0.0	0.0	0.0	0.0016452763601551084	0.0015424465876454142
24	0.0	0.0	0.0	0.0027764038577617455	0.0015938614739002613
25	0.0	0.0	0.0	0.003393382492819911	0.0015938614739002613
26	0.0	0.0	0.0	0.004524509990426548	0.0016452763601551084
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	435	0.0	15.447804	29
TCTAGCG	445	0.0	15.100273	28
CGTTTTT	370	0.0	14.702254	30
TACCGTC	450	0.0	14.576959	7
TCTTATA	165	4.820322E-10	14.551371	2
TCCTACA	760	0.0	14.532224	2
GTCCTAA	485	0.0	14.530338	1
ACCGTCG	410	0.0	14.438211	8
GTATATG	200	3.6379788E-12	14.414756	1
TTAGGAC	590	0.0	14.372073	3
AGGCCCG	720	0.0	14.222519	10
CTATTCC	725	0.0	14.121529	4
GTCCTAC	685	0.0	14.028959	1
CGTCGTA	400	0.0	14.000293	10
AACCGCG	355	0.0	13.9710455	7
CGTTTAT	450	0.0	13.866243	31
ACGTTTT	370	0.0	13.837416	29
CCTATTC	740	0.0	13.837059	3
GTATCAA	5720	0.0	13.748341	1
AACGAAT	210	7.2759576E-12	13.713867	31
>>END_MODULE