FastQCFastQC Report
Thu 2 Feb 2017
SRR4062742_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062742_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1944962
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT59780.3073581900314762No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT43850.2254542762275047No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA25730.1322905023337217No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG25450.13085088551858598No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA24490.12591505643812065No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT23680.12175045065147803No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA23070.11861414258993236No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC22020.1132155795331734No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA21670.11141605851425374No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC21200.10899955886027593No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG20630.10606891034374966No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC20060.10313826182722335No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG19900.1023156236471458No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT19840.10200713432961674No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG19660.10108166637702946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTA1800.016.8902058
CCGTTTA1850.016.43202227
GGACCGT2950.016.2724536
GACCGTT2200.016.0012477
TACGGCC1601.8189894E-1115.99960119
CTAGCGG4100.015.21913229
GCGATTA859.439536E-515.05844930
CCGTTAT1603.0559022E-1015.0003989
ATACGAC756.2446314E-414.9341133
CGCCGTT2150.014.8833525
GTCCTAC6850.014.4825091
GTAGGAC7900.014.3804973
GTATATA2450.014.3680961
CATCGTT3900.014.35861628
TGTAGGA8150.014.3316682
ATAACGA2700.014.221868512
ATCACGA5200.014.15349425
TCCTACA8000.014.0003712
TAGCGTA4250.013.9304977
CTTATAC1503.1708623E-813.8673913
Produced by FastQC (version 0.11.5)