##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062742_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1944962
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.152653368034954	32.0	32.0	32.0	32.0	32.0
2	31.224458369880747	32.0	32.0	32.0	32.0	32.0
3	31.335098577761418	32.0	32.0	32.0	32.0	32.0
4	31.440900644845502	32.0	32.0	32.0	32.0	32.0
5	31.351240281301127	32.0	32.0	32.0	32.0	32.0
6	34.866478111140474	36.0	36.0	36.0	32.0	36.0
7	34.89950497747514	36.0	36.0	36.0	36.0	36.0
8	34.84099637936371	36.0	36.0	36.0	32.0	36.0
9	34.958190956944144	36.0	36.0	36.0	32.0	36.0
10	34.788099716087	36.0	36.0	36.0	32.0	36.0
11	34.9766535284494	36.0	36.0	36.0	36.0	36.0
12	34.869428297313775	36.0	36.0	36.0	32.0	36.0
13	34.92984695844957	36.0	36.0	36.0	32.0	36.0
14	34.87430962661481	36.0	36.0	36.0	32.0	36.0
15	34.83150827625424	36.0	36.0	36.0	32.0	36.0
16	34.85327476835023	36.0	36.0	36.0	32.0	36.0
17	34.81492748958591	36.0	36.0	36.0	32.0	36.0
18	34.81302822368766	36.0	36.0	36.0	32.0	36.0
19	34.803944241584155	36.0	36.0	36.0	32.0	36.0
20	34.78954601683735	36.0	36.0	36.0	32.0	36.0
21	34.776369409787954	36.0	36.0	36.0	32.0	36.0
22	34.7539036752389	36.0	36.0	36.0	32.0	36.0
23	34.69196107687451	36.0	36.0	36.0	32.0	36.0
24	34.6711174819868	36.0	36.0	36.0	32.0	36.0
25	34.65298242330699	36.0	36.0	36.0	32.0	36.0
26	34.58376770343071	36.0	36.0	36.0	32.0	36.0
27	34.57240552771725	36.0	36.0	36.0	32.0	36.0
28	34.530666408906704	36.0	36.0	36.0	32.0	36.0
29	34.48719923576913	36.0	36.0	36.0	32.0	36.0
30	34.47033361063095	36.0	36.0	36.0	32.0	36.0
31	34.4614177552055	36.0	36.0	36.0	32.0	36.0
32	34.42895645261964	36.0	36.0	36.0	32.0	36.0
33	34.3882728814239	36.0	36.0	36.0	32.0	36.0
34	34.37976011870669	36.0	36.0	36.0	32.0	36.0
35	34.35265521897086	36.0	36.0	36.0	32.0	36.0
36	34.31191611969797	36.0	36.0	36.0	32.0	36.0
37	34.2909496432321	36.0	36.0	36.0	32.0	36.0
38	33.8540110295214	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	35.0
22	123.0
23	459.0
24	1335.0
25	3263.0
26	6976.0
27	13634.0
28	23291.0
29	37631.0
30	57245.0
31	83290.0
32	118828.0
33	191696.0
34	459029.0
35	948124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.43239324220373	18.75655602496123	12.350111272455027	26.46093946038001
2	15.55558183428871	20.88843940829662	37.22784158654081	26.328137170873863
3	17.606102330019816	25.553044224000267	28.96956341563486	27.871290030345065
4	12.079586253489149	16.6168027192347	36.35865846015263	34.94495256712352
5	14.132417051035983	37.229743938310335	32.968887293884045	15.66895171676964
6	33.308996903789186	36.32052722989391	16.95249974549579	13.417976120821118
7	29.694054618230243	30.783128658503667	21.199513613425502	18.323303109840587
8	28.254162002800122	32.695534051067476	19.415532899347376	19.63477104678503
9	28.182760514251747	13.789768438631816	18.350246081514374	39.67722496560206
10	16.182077875603873	26.32605087642446	31.08072812229427	26.411143125677395
11	37.40541735651923	20.9244367203809	22.571187643326514	19.098958279773353
12	25.222948144408186	23.509184512372755	28.547388402531876	22.72047894068718
13	29.744652583757592	19.430959434599615	25.230416286624184	25.59397169501861
14	24.071678521225607	19.816531119888204	24.8947280203932	31.21706233849299
15	25.50270905035677	27.12171240363565	22.249792026785098	25.12578651922248
16	25.76163441753618	25.39273260865765	23.863293987234712	24.98233898657146
17	24.16772152874966	25.600500164013486	25.214888517102136	25.016889790134716
18	25.1243983172936	24.234560880880963	26.60874608347104	24.032294718354393
19	25.96266662279263	24.52963091309753	25.33617623377732	24.17152623033252
20	26.203082630920292	23.780618850136918	25.083831972038528	24.93246654690426
21	27.37387915432708	23.742390589009542	24.16034262915433	24.723387627509048
22	26.111590983876276	24.132372902270482	24.698245722277065	25.05779039157618
23	24.325911240288356	23.860348727145407	25.515396468511675	26.298343564054566
24	25.176584426842275	24.752205955694766	25.073086260811266	24.998123356651696
25	25.180697989365324	24.150135889823844	25.294890686585518	25.374275434225314
26	24.890871903924086	24.977146083059722	25.63962689245343	24.492355120562767
27	25.670347976307994	24.446415350444227	24.75603611385324	25.127200559394534
28	24.614671134963046	24.416723822881885	25.472065778148878	25.496539264006184
29	24.826346221674253	24.349113247456764	25.45926347147142	25.365277059397563
30	24.556418068836305	24.603771179077018	25.94708791225741	24.89272283982926
31	25.29483866522842	24.355540108238618	24.67909398744037	25.67052723909259
32	24.835498071427615	24.696369389222	24.59677875454636	25.871353784804025
33	24.51436069187984	24.21851943636945	25.48754165891159	25.779578212839123
34	25.247022821011413	24.313174241964624	25.692224321092134	24.74757861593183
35	26.061434619288192	24.198313386071295	25.359981326113314	24.380270668527203
36	24.88423938359721	24.928044866686342	24.935808514510825	25.25190723520562
37	25.843178427136365	24.777450664845894	24.639967259000432	24.73940364901731
38	25.025296150049357	24.604053553800593	25.174810793024022	25.195839503126027
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	315.0
1	288.0
2	261.0
3	261.0
4	822.0
5	1383.0
6	1383.0
7	1629.0
8	1875.0
9	1820.5
10	1766.0
11	1766.0
12	2313.0
13	2860.0
14	3568.0
15	4276.0
16	4276.0
17	6731.5
18	9187.0
19	9187.0
20	10611.0
21	12035.0
22	12569.0
23	13103.0
24	13103.0
25	15175.0
26	17247.0
27	17247.0
28	22837.5
29	28428.0
30	35888.5
31	43349.0
32	43349.0
33	56309.5
34	69270.0
35	69270.0
36	75888.0
37	82506.0
38	93435.0
39	104364.0
40	104364.0
41	110673.5
42	116983.0
43	132448.5
44	147914.0
45	147914.0
46	154704.5
47	161495.0
48	161495.0
49	172179.0
50	182863.0
51	183317.0
52	183771.0
53	183771.0
54	177112.5
55	170454.0
56	170454.0
57	165557.5
58	160661.0
59	143790.5
60	126920.0
61	126920.0
62	119639.0
63	112358.0
64	92488.5
65	72619.0
66	72619.0
67	60210.0
68	47801.0
69	47801.0
70	38287.0
71	28773.0
72	22248.5
73	15724.0
74	15724.0
75	11705.0
76	7686.0
77	7686.0
78	7581.0
79	7476.0
80	5696.5
81	3917.0
82	3917.0
83	3733.5
84	3550.0
85	3550.0
86	2225.0
87	900.0
88	767.0
89	634.0
90	634.0
91	378.5
92	123.0
93	93.5
94	64.0
95	64.0
96	47.5
97	31.0
98	31.0
99	25.5
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009563168843401568
2	5.141488625484714E-5
3	0.0
4	1.542446587645414E-4
5	5.141488625484714E-5
6	2.0565954501938855E-4
7	3.5990420378392997E-4
8	0.002313669881468121
9	0.004010361127878077
10	7.198084075678599E-4
11	0.005244318397994408
12	1.542446587645414E-4
13	2.0565954501938855E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.0282977250969428E-4
22	1.0282977250969428E-4
23	1.542446587645414E-4
24	0.0
25	2.0565954501938855E-4
26	0.0
27	1.0282977250969428E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.0282977250969428E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1944962.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.97534395864546
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.6347012554552	34.590154187671104
2	14.767460547388225	13.874130772185774
3	5.059714155511001	7.130454383626699
4	2.238179812551227	4.20557066143562
5	1.1947029642908977	2.8060791337989124
6	0.7251605846289164	2.0438800732917466
7	0.45898512852962664	1.5092688999207615
8	0.3189386206336975	1.1985801124771105
9	0.24608246009349188	1.0403827384573283
>10	1.1579602565603928	9.561899520280905
>50	0.08812130841810006	2.9064407525881197
>100	0.08894322260907964	9.141094269944038
>500	0.013996386298091135	4.544205407335478
>1k	0.006943089265982215	5.138375174242028
>5k	1.1020776612670184E-4	0.309483912744389
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5978	0.3073581900314762	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4385	0.2254542762275047	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2573	0.1322905023337217	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2545	0.13085088551858598	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2449	0.12591505643812065	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2368	0.12175045065147803	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2307	0.11861414258993236	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2202	0.1132155795331734	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2167	0.11141605851425374	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2120	0.10899955886027593	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2063	0.10606891034374966	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2006	0.10313826182722335	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1990	0.1023156236471458	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1984	0.10200713432961674	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1966	0.10108166637702946	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0282977250969428E-4
2	0.0	0.0	0.0	0.0	1.0282977250969428E-4
3	0.0	0.0	0.0	0.0	1.0282977250969428E-4
4	0.0	0.0	0.0	0.0	1.0282977250969428E-4
5	0.0	0.0	0.0	0.0	1.0282977250969428E-4
6	0.0	0.0	0.0	0.0	2.0565954501938855E-4
7	0.0	0.0	0.0	5.141488625484714E-5	2.0565954501938855E-4
8	0.0	0.0	0.0	5.141488625484714E-5	2.0565954501938855E-4
9	0.0	0.0	0.0	5.141488625484714E-5	2.0565954501938855E-4
10	0.0	0.0	0.0	1.0282977250969428E-4	2.0565954501938855E-4
11	0.0	0.0	0.0	1.0282977250969428E-4	2.570744312742357E-4
12	0.0	0.0	0.0	1.0282977250969428E-4	7.712232938227071E-4
13	0.0	0.0	0.0	1.0282977250969428E-4	7.712232938227071E-4
14	0.0	0.0	0.0	1.0282977250969428E-4	7.712232938227071E-4
15	0.0	0.0	0.0	1.0282977250969428E-4	9.254679525872485E-4
16	0.0	0.0	0.0	1.5424465876454142E-4	0.0011825423838614842
17	1.0282977250969428E-4	0.0	0.0	2.0565954501938855E-4	0.0011825423838614842
18	1.0282977250969428E-4	0.0	0.0	2.570744312742357E-4	0.0012339572701163314
19	1.0282977250969428E-4	0.0	0.0	3.0848931752908284E-4	0.0012339572701163314
20	1.0282977250969428E-4	0.0	0.0	3.5990420378392997E-4	0.0013882019288808728
21	1.0282977250969428E-4	0.0	0.0	5.141488625484714E-4	0.0014396168151357199
22	1.0282977250969428E-4	0.0	0.0	7.198084075678599E-4	0.001491031701390567
23	1.0282977250969428E-4	0.0	0.0	0.0016966912464099555	0.001491031701390567
24	1.0282977250969428E-4	0.0	0.0	0.0028278187440165924	0.001491031701390567
25	1.0282977250969428E-4	0.0	0.0	0.003393382492819911	0.001491031701390567
26	1.0282977250969428E-4	0.0	0.0	0.004524509990426548	0.0015424465876454142
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTTA	180	0.0	16.890205	8
CCGTTTA	185	0.0	16.432022	27
GGACCGT	295	0.0	16.272453	6
GACCGTT	220	0.0	16.001247	7
TACGGCC	160	1.8189894E-11	15.999601	19
CTAGCGG	410	0.0	15.219132	29
GCGATTA	85	9.439536E-5	15.058449	30
CCGTTAT	160	3.0559022E-10	15.000398	9
ATACGAC	75	6.2446314E-4	14.934113	3
CGCCGTT	215	0.0	14.88335	25
GTCCTAC	685	0.0	14.482509	1
GTAGGAC	790	0.0	14.380497	3
GTATATA	245	0.0	14.368096	1
CATCGTT	390	0.0	14.358616	28
TGTAGGA	815	0.0	14.331668	2
ATAACGA	270	0.0	14.2218685	12
ATCACGA	520	0.0	14.153494	25
TCCTACA	800	0.0	14.000371	2
TAGCGTA	425	0.0	13.930497	7
CTTATAC	150	3.1708623E-8	13.867391	3
>>END_MODULE