FastQCFastQC Report
Thu 2 Feb 2017
SRR4062741_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062741_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2849071
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA80900.2839522075792425No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC63560.22309026345780783No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT60970.21399958091602492No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT40710.14288868195983884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATAC2550.015.683723
GTTATTC3450.015.3018543
GCGAGAA2650.015.09426520
TGGCGAG3550.014.87212318
CTAGCGG5500.014.8357729
TCTAGCG5550.014.70159828
CGTTATT3050.014.6938682
ACCGTCG3200.014.4986558
AGTATCG800.001019309213.9987028
TAGCGGC5750.013.91224230
ATACCGT4050.013.8244226
CATCGTT4750.013.80948828
ATCGTTT5000.013.75944929
TACCGTC4000.013.598747
CGTTTAT5200.013.53768131
TCGACGC1554.879257E-813.41976114
TTCGTTC6150.013.26776129
CTTTCGT6650.013.23185627
CGTCGTA3900.013.12837110
TTTCGTT6500.013.04517428
Produced by FastQC (version 0.11.5)