##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062741_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2849071
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.209852615115594	32.0	32.0	32.0	32.0	32.0
2	30.755846028407156	32.0	32.0	32.0	32.0	32.0
3	30.82142916059305	32.0	32.0	32.0	32.0	32.0
4	30.89479553159609	32.0	32.0	32.0	32.0	32.0
5	30.76971546163644	32.0	32.0	32.0	32.0	32.0
6	34.44439187370199	36.0	36.0	36.0	32.0	36.0
7	34.37891228403925	36.0	36.0	36.0	32.0	36.0
8	34.355354780558294	36.0	36.0	36.0	32.0	36.0
9	34.52246539310533	36.0	36.0	36.0	32.0	36.0
10	34.19001983453554	36.0	36.0	36.0	32.0	36.0
11	34.524512727131054	36.0	36.0	36.0	32.0	36.0
12	34.31574327210519	36.0	36.0	36.0	32.0	36.0
13	34.40857633944538	36.0	36.0	36.0	32.0	36.0
14	34.2963987208462	36.0	36.0	36.0	32.0	36.0
15	34.22854853389052	36.0	36.0	36.0	32.0	36.0
16	34.2472844657083	36.0	36.0	36.0	32.0	36.0
17	34.177892723628155	36.0	36.0	36.0	32.0	36.0
18	34.20426272283141	36.0	36.0	36.0	32.0	36.0
19	34.193843186077146	36.0	36.0	36.0	32.0	36.0
20	34.17103785760341	36.0	36.0	36.0	32.0	36.0
21	34.143893921913495	36.0	36.0	36.0	32.0	36.0
22	34.11704903107013	36.0	36.0	36.0	32.0	36.0
23	34.0418792652061	36.0	36.0	36.0	32.0	36.0
24	34.02594494837089	36.0	36.0	36.0	32.0	36.0
25	33.995267580204214	36.0	36.0	36.0	32.0	36.0
26	33.94494872188162	36.0	36.0	36.0	32.0	36.0
27	33.936924000840975	36.0	36.0	36.0	32.0	36.0
28	33.9083634630376	36.0	36.0	36.0	32.0	36.0
29	33.878062357870334	36.0	36.0	36.0	32.0	36.0
30	33.833193346181965	36.0	36.0	36.0	32.0	36.0
31	33.858477026371055	36.0	36.0	36.0	32.0	36.0
32	33.80467913927031	36.0	36.0	36.0	32.0	36.0
33	33.77020825384836	36.0	36.0	36.0	32.0	36.0
34	33.77585044388153	36.0	36.0	36.0	32.0	36.0
35	33.715838601424814	36.0	36.0	36.0	27.0	36.0
36	33.667293303676885	36.0	36.0	36.0	27.0	36.0
37	33.65281068811553	36.0	36.0	36.0	27.0	36.0
38	33.03664282146707	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	11.0
8	8.0
9	8.0
10	24.0
11	35.0
12	17.0
13	10.0
14	306.0
15	704.0
16	947.0
17	1138.0
18	1604.0
19	2048.0
20	2657.0
21	3833.0
22	5710.0
23	8988.0
24	13465.0
25	20371.0
26	29837.0
27	43048.0
28	60872.0
29	82511.0
30	110407.0
31	147809.0
32	197413.0
33	289578.0
34	617547.0
35	1208165.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.27912998373604	17.23086806234001	11.781493669426995	25.70850828449695
2	16.005689300953485	19.89320269012625	38.416758854413594	25.684349154506663
3	18.79780223792052	24.652720734534437	28.43547759762644	28.1139994299186
4	12.215057114937494	15.987970482191596	36.85650924958722	34.94046315328369
5	14.09087335349434	37.136582400395355	34.266376606492514	14.506167639617784
6	33.270201333283254	35.98667910119032	17.67728760779444	13.06583195773198
7	29.588978302050034	30.77754117043766	21.59321406872626	18.04026645878604
8	27.926558104353898	33.227859764027016	19.93081916635516	18.91476296526392
9	27.446111230253596	14.84410839633943	18.515596180217848	39.19418419318913
10	15.11971718272209	27.283710207760624	32.49597760374694	25.100595005770348
11	36.83876891713736	21.6134831681528	22.529136498228855	19.018611416480987
12	24.261431503293622	23.87547029808097	29.60535852868612	22.257739669939287
13	29.53281788901858	20.082213660737008	25.321316050444505	25.063652399799906
14	23.5672686847512	19.844236552353316	25.805152823620553	30.783341939274933
15	25.113010495687416	27.187783491690798	23.189093697251238	24.51011231537055
16	25.50541430071835	26.137550759069235	24.32349323493235	24.03354170528007
17	23.826207722642348	25.90724670447161	25.996873421307708	24.269672151578334
18	24.673481186008576	25.132989132361526	26.893623085544437	23.29990659608546
19	24.92724239278893	26.105018774288368	25.782185961107718	23.18555287181498
20	25.074072163012463	24.618740283568002	26.14665744208928	24.16053011133026
21	26.100919234565577	25.10937585559579	24.646037828039812	24.14366708179882
22	24.88621788458987	25.17040113504812	25.48070003994572	24.46268094041629
23	23.771313836200918	24.98115870043299	25.659901818137143	25.587625645228947
24	23.983030617904557	25.97796858873997	25.55956133761137	24.479439455744103
25	24.78664943245765	24.924998192093092	25.363599918275586	24.924752457173668
26	23.915874067372783	25.60105373315935	26.05546211584254	24.427610083625325
27	25.020210001484816	25.61245167764951	25.25402471254059	24.113313608325083
28	24.196812804942258	25.01418091193092	26.053915546351224	24.735090736775597
29	23.943786068324464	25.781603223858802	26.1635237787669	24.111086929049833
30	24.465992155073316	25.43707824743987	26.219535095955564	23.877394501531253
31	24.272958015126502	25.510730957521606	25.31023301216923	24.906078015182665
32	23.985402018402276	25.769537366697943	25.31697466414077	24.928085950759016
33	23.922136456018787	25.213333192924814	25.7227704004802	25.141759950576205
34	24.266787047837827	25.72210273423692	26.079244463057034	23.931865754868223
35	25.22764619503765	25.225013331067903	25.78953447103481	23.757806002859642
36	24.097501692030747	26.01823742350757	25.562793297030733	24.321467587430952
37	25.104876719176104	25.75690083722354	25.420410759398358	23.71781168420199
38	24.138308675304142	25.780695236025934	25.522736295338945	24.55825979333098
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	193.0
1	282.5
2	372.0
3	372.0
4	938.5
5	1505.0
6	1505.0
7	1777.0
8	2049.0
9	2123.5
10	2198.0
11	2198.0
12	2922.0
13	3646.0
14	4795.5
15	5945.0
16	5945.0
17	8944.0
18	11943.0
19	11943.0
20	15087.0
21	18231.0
22	21317.5
23	24404.0
24	24404.0
25	30223.0
26	36042.0
27	36042.0
28	46246.5
29	56451.0
30	67591.5
31	78732.0
32	78732.0
33	94272.5
34	109813.0
35	109813.0
36	122541.5
37	135270.0
38	152594.5
39	169919.0
40	169919.0
41	181542.0
42	193165.0
43	214631.5
44	236098.0
45	236098.0
46	251245.0
47	266392.0
48	266392.0
49	267964.5
50	269537.0
51	260534.0
52	251531.0
53	251531.0
54	238552.0
55	225573.0
56	225573.0
57	215199.5
58	204826.0
59	183300.5
60	161775.0
61	161775.0
62	149876.0
63	137977.0
64	114905.0
65	91833.0
66	91833.0
67	75892.5
68	59952.0
69	59952.0
70	48528.0
71	37104.0
72	29071.0
73	21038.0
74	21038.0
75	16076.0
76	11114.0
77	11114.0
78	10009.0
79	8904.0
80	7019.0
81	5134.0
82	5134.0
83	4954.5
84	4775.0
85	4775.0
86	3251.5
87	1728.0
88	1487.5
89	1247.0
90	1247.0
91	932.0
92	617.0
93	531.0
94	445.0
95	445.0
96	402.0
97	359.0
98	359.0
99	796.5
100	1234.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12337354878133959
2	0.056895738997027455
3	0.012460201939509407
4	0.002772833671045755
5	2.4569412275088966E-4
6	4.913882455017793E-4
7	0.0
8	5.615865662877478E-4
9	2.807932831438739E-4
10	8.072806890386375E-4
11	0.002351643746329944
12	0.002807932831438739
13	0.013899267515621759
14	0.00926617834374784
15	0.02327074334054855
16	0.011196632165361972
17	0.020708504631860702
18	0.005405270700519573
19	0.008002608569600406
20	0.005615865662877478
21	0.006282749710344178
22	0.007195327880561769
23	0.00968736826846365
24	0.014425754921516522
25	0.016601902865881545
26	0.018672753329067617
27	0.008213203531958311
28	0.006001956427200305
29	0.010915838882218098
30	0.003369519397726487
31	0.005756262304449415
32	0.0064582455123091
33	0.008458897654709202
34	0.01200391285440061
35	0.016215812101558716
36	0.014846944846232333
37	0.00877479009824606
38	0.005896658946021352
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2849071.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.36311526186383
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.74739049397931	35.41674706692255
2	15.298029662322184	15.103168030012437
3	5.754920522582717	8.522424152373489
4	2.6844014835903334	5.300416793743516
5	1.415862513361407	3.494569222100567
6	0.8498207855288271	2.5169880832792297
7	0.5267075872979907	1.8199949138762248
8	0.3600581863124115	1.421887500153377
9	0.22356394836003782	0.9932231656164529
>10	0.9890361482354892	8.425200548776724
>50	0.07084569324337535	2.436772493490678
>100	0.06505999490326878	6.87773419117134
>500	0.00929693718371634	3.186427608857397
>1k	0.0047914983946499914	3.759237588466097
>5k	2.145447042380593E-4	0.7252086411601216
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	8090	0.2839522075792425	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	6356	0.22309026345780783	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6097	0.21399958091602492	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4071	0.14288868195983884	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	3.509916039298424E-5	0.0
6	0.0	0.0	0.0	3.509916039298424E-5	0.0
7	0.0	0.0	0.0	7.019832078596848E-5	0.0
8	0.0	0.0	0.0	7.019832078596848E-5	0.0
9	3.509916039298424E-5	0.0	0.0	7.019832078596848E-5	0.0
10	3.509916039298424E-5	0.0	0.0	7.019832078596848E-5	0.0
11	3.509916039298424E-5	0.0	0.0	1.0529748117895271E-4	0.0
12	3.509916039298424E-5	0.0	0.0	1.4039664157193695E-4	0.0
13	3.509916039298424E-5	0.0	0.0	1.4039664157193695E-4	0.0
14	3.509916039298424E-5	0.0	0.0	1.754958019649212E-4	0.0
15	3.509916039298424E-5	0.0	0.0	2.4569412275088966E-4	0.0
16	3.509916039298424E-5	0.0	0.0	3.509916039298424E-4	0.0
17	3.509916039298424E-5	0.0	0.0	4.2118992471581085E-4	0.0
18	3.509916039298424E-5	0.0	0.0	4.562890851087951E-4	0.0
19	3.509916039298424E-5	0.0	0.0	5.966857266807321E-4	0.0
20	3.509916039298424E-5	3.509916039298424E-5	0.0	6.668840474667006E-4	0.0
21	3.509916039298424E-5	3.509916039298424E-5	0.0	8.072806890386375E-4	0.0
22	3.509916039298424E-5	3.509916039298424E-5	0.0	0.0011231731325754956	0.0
23	3.509916039298424E-5	3.509916039298424E-5	0.0	0.002211247104758007	0.0
24	3.509916039298424E-5	3.509916039298424E-5	0.0	0.003053626954189629	0.0
25	3.509916039298424E-5	3.509916039298424E-5	0.0	0.0038609076432282665	0.0
26	3.509916039298424E-5	3.509916039298424E-5	0.0	0.004984080775803762	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAATAC	255	0.0	15.68372	3
GTTATTC	345	0.0	15.301854	3
GCGAGAA	265	0.0	15.094265	20
TGGCGAG	355	0.0	14.872123	18
CTAGCGG	550	0.0	14.83577	29
TCTAGCG	555	0.0	14.701598	28
CGTTATT	305	0.0	14.693868	2
ACCGTCG	320	0.0	14.498655	8
AGTATCG	80	0.0010193092	13.998702	8
TAGCGGC	575	0.0	13.912242	30
ATACCGT	405	0.0	13.824422	6
CATCGTT	475	0.0	13.809488	28
ATCGTTT	500	0.0	13.759449	29
TACCGTC	400	0.0	13.59874	7
CGTTTAT	520	0.0	13.537681	31
TCGACGC	155	4.879257E-8	13.419761	14
TTCGTTC	615	0.0	13.267761	29
CTTTCGT	665	0.0	13.231856	27
CGTCGTA	390	0.0	13.128371	10
TTTCGTT	650	0.0	13.045174	28
>>END_MODULE