FastQCFastQC Report
Thu 2 Feb 2017
SRR4062741_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062741_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2849071
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA83100.291674022865699No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC78450.27535291328296135No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT65810.23098757454622929No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT45930.1612104436849766No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG30940.10859680225589324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC3050.016.788591
CGTCGTA5000.016.3205110
ATCGTTT5100.015.99937729
TACCGTC5300.015.398067
ATACCGT5550.015.2808296
ACCGTCG5150.015.2251138
CTAGCGG5700.015.15730629
CGTTTAT5550.014.99040731
TACACCG1401.27947715E-814.8583895
CCGTCGT5300.014.79369459
GTCGTAG5300.014.79291511
TAGCGGC5950.014.52044330
CATCGTT5400.014.51795228
TGTACGG800.001017949414.0011745
TAGCGTA4500.013.8683187
ATAGCGT4750.013.8119276
AATAGCG5100.013.805085
CCGATAA2900.013.7942619
TACGCTA5800.013.7942619
TCTAGCG6050.013.75153128
Produced by FastQC (version 0.11.5)