##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062741_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2849071
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14123551150533	32.0	32.0	32.0	32.0	32.0
2	31.207201926522714	32.0	32.0	32.0	32.0	32.0
3	31.338288515800414	32.0	32.0	32.0	32.0	32.0
4	31.453278981113492	32.0	32.0	32.0	32.0	32.0
5	31.359231833815304	32.0	32.0	32.0	32.0	32.0
6	34.886859611431234	36.0	36.0	36.0	36.0	36.0
7	34.9209661675683	36.0	36.0	36.0	36.0	36.0
8	34.85015501544188	36.0	36.0	36.0	32.0	36.0
9	34.979316766763624	36.0	36.0	36.0	36.0	36.0
10	34.80998543033852	36.0	36.0	36.0	32.0	36.0
11	35.01166696091463	36.0	36.0	36.0	36.0	36.0
12	34.902581578346066	36.0	36.0	36.0	32.0	36.0
13	34.955235232818	36.0	36.0	36.0	36.0	36.0
14	34.89806010450424	36.0	36.0	36.0	32.0	36.0
15	34.8589810503143	36.0	36.0	36.0	32.0	36.0
16	34.87316532301231	36.0	36.0	36.0	32.0	36.0
17	34.85009148596156	36.0	36.0	36.0	32.0	36.0
18	34.840460627341336	36.0	36.0	36.0	32.0	36.0
19	34.846018579389565	36.0	36.0	36.0	32.0	36.0
20	34.8301418251774	36.0	36.0	36.0	32.0	36.0
21	34.81707686470432	36.0	36.0	36.0	32.0	36.0
22	34.79120843250308	36.0	36.0	36.0	32.0	36.0
23	34.71924988882341	36.0	36.0	36.0	32.0	36.0
24	34.69852172866173	36.0	36.0	36.0	32.0	36.0
25	34.67588241921665	36.0	36.0	36.0	32.0	36.0
26	34.60646926664867	36.0	36.0	36.0	32.0	36.0
27	34.57963911745267	36.0	36.0	36.0	32.0	36.0
28	34.53656191790236	36.0	36.0	36.0	32.0	36.0
29	34.503885652551304	36.0	36.0	36.0	32.0	36.0
30	34.479538768953105	36.0	36.0	36.0	32.0	36.0
31	34.47009604183258	36.0	36.0	36.0	32.0	36.0
32	34.44253161820116	36.0	36.0	36.0	32.0	36.0
33	34.40020097779241	36.0	36.0	36.0	32.0	36.0
34	34.38577522287089	36.0	36.0	36.0	32.0	36.0
35	34.34338386091466	36.0	36.0	36.0	32.0	36.0
36	34.319386213962375	36.0	36.0	36.0	32.0	36.0
37	34.292487270412	36.0	36.0	36.0	32.0	36.0
38	33.85199596640449	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	11.0
21	56.0
22	196.0
23	669.0
24	1868.0
25	4581.0
26	9984.0
27	19139.0
28	34161.0
29	54350.0
30	83053.0
31	119824.0
32	172646.0
33	277596.0
34	661429.0
35	1409505.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.59068277806484	18.44089086334329	12.515690640936356	26.452735717655518
2	15.079652279637818	20.937210760981387	37.5957987708976	26.387338188483195
3	17.736413027263975	25.40242064869566	28.850035678296535	28.011130645743826
4	11.795141069186606	16.939323560061677	36.76857970497669	34.49695566577503
5	13.65449555626768	37.6167091776296	33.93125965008218	14.797535616020532
6	33.38122037186923	36.43471228816742	17.233612699740895	12.950454640222445
7	29.335911024720073	30.98315550281303	21.542606493858855	18.138326978608042
8	27.85144687558902	33.527436459306266	19.65239745440595	18.96871921069877
9	27.282220340059858	14.59724552987414	18.307359609739525	39.81317452032648
10	14.893116666274722	27.580824565178247	32.51329131451601	25.01276745403102
11	37.03859967370274	21.534706290348396	22.36863165757321	19.058062378375645
12	24.120762637501493	23.995001860262683	29.543042266572133	22.34119323566369
13	29.500980671574002	20.072177989433737	25.330388744670184	25.096452594322074
14	23.455856312461147	19.824918368127715	25.684161609170147	31.03506371024099
15	25.014750422155153	27.315956675000375	23.06843178004339	24.60086112280108
16	25.41766260569238	26.160922687052267	24.22927481599259	24.192139891262762
17	23.706008028581948	26.12342760148835	25.889877788233427	24.28068658169628
18	24.697805003806504	25.157253013350665	26.781501759696408	23.363440223146423
19	24.95027326451324	26.179445861475546	25.666471632332083	23.20380924167913
20	25.14012462307889	24.626799402331496	26.102929691818844	24.13014628277077
21	25.980486959073296	25.16327263397271	24.687889271898996	24.168351135054994
22	24.78719356645752	25.17925160087439	25.51234298374065	24.521211848927436
23	23.758355811467975	24.889463736348592	25.69930836958792	25.65287208259552
24	23.916006305213173	25.991244163448368	25.551556981205454	24.541192550133008
25	24.741468359335375	24.902608604699566	25.405860366414174	24.95006266955088
26	24.054963881208998	25.504769800401604	25.99134946092955	24.448916857459853
27	25.11314218323874	25.505305239955494	25.214719189068713	24.166833387737054
28	24.144115748607177	25.01945370964781	26.042032648537017	24.794397893207996
29	23.988801963868223	25.589534272750665	26.19639875594536	24.225265007435755
30	24.519185376566607	25.278450414187642	26.202962298938846	23.999401910306904
31	24.32006783965721	25.361319531875477	25.317621077186214	25.000991551281103
32	24.05854399556908	25.63895389058398	25.36546123280185	24.937040881045082
33	23.919340725450507	25.14637227362884	25.742110322978963	25.192176677941685
34	24.23172325294807	25.595360733375898	26.175269061388782	23.997646952287255
35	25.335732243948993	25.12117107646668	25.719927653610597	23.82316902597373
36	24.205328684332542	25.814028502624186	25.58749852144787	24.3931442915954
37	25.20007398903011	25.624142044898147	25.399612715864222	23.776171250207522
38	24.263961315082224	25.459720350753173	25.570598676269334	24.705719657895266
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	96.0
1	147.5
2	199.0
3	199.0
4	697.5
5	1196.0
6	1196.0
7	1528.5
8	1861.0
9	1988.0
10	2115.0
11	2115.0
12	2742.0
13	3369.0
14	4613.0
15	5857.0
16	5857.0
17	9098.0
18	12339.0
19	12339.0
20	15269.0
21	18199.0
22	21294.5
23	24390.0
24	24390.0
25	30460.5
26	36531.0
27	36531.0
28	46539.0
29	56547.0
30	67524.0
31	78501.0
32	78501.0
33	94711.0
34	110921.0
35	110921.0
36	123107.5
37	135294.0
38	152107.5
39	168921.0
40	168921.0
41	180078.0
42	191235.0
43	212160.5
44	233086.0
45	233086.0
46	251549.0
47	270012.0
48	270012.0
49	272167.0
50	274322.0
51	263091.5
52	251861.0
53	251861.0
54	238780.0
55	225699.0
56	225699.0
57	215827.0
58	205955.0
59	184240.5
60	162526.0
61	162526.0
62	150565.5
63	138605.0
64	115088.5
65	91572.0
66	91572.0
67	75797.5
68	60023.0
69	60023.0
70	48409.5
71	36796.0
72	28829.5
73	20863.0
74	20863.0
75	15661.0
76	10459.0
77	10459.0
78	9667.0
79	8875.0
80	6823.0
81	4771.0
82	4771.0
83	4397.0
84	4023.0
85	4023.0
86	2549.0
87	1075.0
88	895.5
89	716.0
90	716.0
91	434.0
92	152.0
93	106.0
94	60.0
95	60.0
96	47.0
97	34.0
98	34.0
99	24.5
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008388699333923234
2	0.0
3	0.0
4	2.807932831438739E-4
5	7.019832078596848E-5
6	1.754958019649212E-4
7	3.509916039298424E-4
8	0.002456941227508897
9	0.003580114360084392
10	0.0010529748117895272
11	0.004738386653052873
12	3.8609076432282667E-4
13	1.0529748117895271E-4
14	0.0
15	0.0
16	3.509916039298424E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	7.019832078596848E-5
22	1.0529748117895271E-4
23	2.1059496235790543E-4
24	0.0
25	0.0
26	0.0
27	3.509916039298424E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	7.019832078596848E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2849071.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.905296326695364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.45206233305797	32.576695642322015
2	16.30361619558167	15.294518977009359
3	6.271747086959473	8.825344667997674
4	2.948521030104022	5.532050105700888
5	1.5589401293943796	3.656127436241009
6	0.95279237921801	2.6814605331022716
7	0.5796124543709763	1.903082574883969
8	0.3850640076482717	1.4449233106789658
9	0.26706608617696304	1.1274132519847089
>10	1.119774335803901	8.997904469839819
>50	0.07503798877855508	2.453364628117252
>100	0.06935832739446492	7.035805699397134
>500	0.010612284482321065	3.4155655817310393
>1k	0.005569567742441576	4.253094035043901
>5k	2.2579328685573959E-4	0.8026490859501481
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	8310	0.291674022865699	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	7845	0.27535291328296135	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6581	0.23098757454622929	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4593	0.1612104436849766	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	3094	0.10859680225589324	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	3.509916039298424E-5	0.0
6	0.0	0.0	0.0	3.509916039298424E-5	0.0
7	0.0	0.0	0.0	3.509916039298424E-5	0.0
8	0.0	0.0	0.0	3.509916039298424E-5	0.0
9	0.0	0.0	0.0	1.0529748117895271E-4	0.0
10	0.0	0.0	0.0	1.0529748117895271E-4	0.0
11	0.0	0.0	0.0	1.4039664157193695E-4	0.0
12	0.0	0.0	0.0	1.754958019649212E-4	0.0
13	0.0	0.0	0.0	1.754958019649212E-4	0.0
14	0.0	0.0	0.0	2.1059496235790543E-4	0.0
15	0.0	0.0	0.0	3.1589244353685814E-4	0.0
16	0.0	0.0	0.0	4.2118992471581085E-4	0.0
17	0.0	0.0	0.0	5.264874058947636E-4	0.0
18	0.0	0.0	0.0	5.966857266807321E-4	0.0
19	0.0	0.0	0.0	7.019832078596848E-4	0.0
20	0.0	0.0	0.0	8.423798494316217E-4	0.0
21	0.0	0.0	0.0	9.476773306105745E-4	0.0
22	0.0	0.0	0.0	0.0011933714533614642	0.0
23	0.0	0.0	0.0	0.0022814454255439755	0.0
24	0.0	0.0	0.0	0.0030185277937966448	0.0
25	0.0	0.0	0.0	0.0038960068036212507	0.0
26	0.0	0.0	0.0	0.004808584973838841	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAC	305	0.0	16.78859	1
CGTCGTA	500	0.0	16.32051	10
ATCGTTT	510	0.0	15.999377	29
TACCGTC	530	0.0	15.39806	7
ATACCGT	555	0.0	15.280829	6
ACCGTCG	515	0.0	15.225113	8
CTAGCGG	570	0.0	15.157306	29
CGTTTAT	555	0.0	14.990407	31
TACACCG	140	1.27947715E-8	14.858389	5
CCGTCGT	530	0.0	14.7936945	9
GTCGTAG	530	0.0	14.792915	11
TAGCGGC	595	0.0	14.520443	30
CATCGTT	540	0.0	14.517952	28
TGTACGG	80	0.0010179494	14.001174	5
TAGCGTA	450	0.0	13.868318	7
ATAGCGT	475	0.0	13.811927	6
AATAGCG	510	0.0	13.80508	5
CCGATAA	290	0.0	13.794261	9
TACGCTA	580	0.0	13.794261	9
TCTAGCG	605	0.0	13.751531	28
>>END_MODULE