FastQCFastQC Report
Thu 2 Feb 2017
SRR4062740_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062740_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4337700
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT71540.16492611291698364No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA71230.1642114484634714No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG70220.1618830255665445No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG68940.15893215298430044No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA67790.15628097839869054No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG66150.15250017290269038No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC63940.14740530695990964No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT62780.14473107868225096No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC61930.14277151485810452No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG61680.142195172556885No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC59360.13684671600156764No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG55450.12783272241049404No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC55130.12709500426493303No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA54400.12541208474537197No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG53490.12331419876893285No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA52050.11999446711390829No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT51630.11902621204785946No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG50300.1159600710053715No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT50050.11538372870415196No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC49420.11393134610507873No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT48880.11268644673444453No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT47230.10888258754639556No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT46800.10789127878829793No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT46360.10687691633815155No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG44260.10203564100790742No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT44050.101551513474883No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC43700.10074463425317563No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT43700.10074463425317563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG12450.016.8342328
GTATCAA74800.015.71725751
CTAGCGG13300.015.638247529
ATACCGT13400.015.5197546
ACCGTCG12850.015.43831358
TACCGTC13500.015.0505137
CTATTCC16350.014.4805564
GTTCAAA22250.014.397341
TCTATAC2800.014.2846083
TAGCGGC14450.014.2829630
GTCCTAT16900.014.2163081
GTATTAC2053.6379788E-1214.0637441
ATCGTTT12800.013.99923929
AGGCCCG20700.013.91340910
CGTCGTA14150.013.90849310
CCGTCGT14450.013.8411949
CATCGTT13050.013.73089528
CCTATTC16900.013.72674753
GTATAAT3750.013.6678751
CGAGCCG20850.013.27643515
Produced by FastQC (version 0.11.5)