##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062740_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4337700
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.266197754570396	32.0	32.0	32.0	32.0	32.0
2	30.833121930977246	32.0	32.0	32.0	32.0	32.0
3	30.87516010789128	32.0	32.0	32.0	32.0	32.0
4	30.95726882910298	32.0	32.0	32.0	32.0	32.0
5	30.84412684141365	32.0	32.0	32.0	32.0	32.0
6	34.513015653456904	36.0	36.0	36.0	32.0	36.0
7	34.44775318717293	36.0	36.0	36.0	32.0	36.0
8	34.42550821864121	36.0	36.0	36.0	32.0	36.0
9	34.5802925513521	36.0	36.0	36.0	32.0	36.0
10	34.27088318694239	36.0	36.0	36.0	32.0	36.0
11	34.55846923484796	36.0	36.0	36.0	32.0	36.0
12	34.357860386840954	36.0	36.0	36.0	32.0	36.0
13	34.45869769693616	36.0	36.0	36.0	32.0	36.0
14	34.34754639555525	36.0	36.0	36.0	32.0	36.0
15	34.28388984945939	36.0	36.0	36.0	32.0	36.0
16	34.30723263480647	36.0	36.0	36.0	32.0	36.0
17	34.21955575535422	36.0	36.0	36.0	32.0	36.0
18	34.249005002651174	36.0	36.0	36.0	32.0	36.0
19	34.24712889319225	36.0	36.0	36.0	32.0	36.0
20	34.2284122922286	36.0	36.0	36.0	32.0	36.0
21	34.21084883694124	36.0	36.0	36.0	32.0	36.0
22	34.18945454964613	36.0	36.0	36.0	32.0	36.0
23	34.122843211840376	36.0	36.0	36.0	32.0	36.0
24	34.10742213615511	36.0	36.0	36.0	32.0	36.0
25	34.08468289646587	36.0	36.0	36.0	32.0	36.0
26	34.03107107453258	36.0	36.0	36.0	32.0	36.0
27	34.037540171058396	36.0	36.0	36.0	32.0	36.0
28	34.01882195633631	36.0	36.0	36.0	32.0	36.0
29	33.993083200774606	36.0	36.0	36.0	32.0	36.0
30	33.96443737464555	36.0	36.0	36.0	32.0	36.0
31	33.98968301173433	36.0	36.0	36.0	32.0	36.0
32	33.93339419508034	36.0	36.0	36.0	32.0	36.0
33	33.89228415980819	36.0	36.0	36.0	32.0	36.0
34	33.920111118795674	36.0	36.0	36.0	32.0	36.0
35	33.87450077229868	36.0	36.0	36.0	32.0	36.0
36	33.83150056481546	36.0	36.0	36.0	32.0	36.0
37	33.83765659220324	36.0	36.0	36.0	32.0	36.0
38	33.22997556308643	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	8.0
8	3.0
9	12.0
10	31.0
11	42.0
12	25.0
13	15.0
14	302.0
15	795.0
16	1071.0
17	1303.0
18	1826.0
19	2398.0
20	3509.0
21	5509.0
22	8650.0
23	13450.0
24	20182.0
25	30607.0
26	44515.0
27	62963.0
28	87824.0
29	116780.0
30	156436.0
31	209744.0
32	283959.0
33	426514.0
34	938829.0
35	1920395.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.81595004229694	16.961037192452796	11.305550073687842	25.917462691562427
2	17.23207713814564	19.34702362265751	36.34308409108786	27.077815148108996
3	18.516054031595747	23.70169829792081	28.316970113664087	29.46527755681936
4	12.772254721187466	15.294194404521974	35.335351051071406	36.598199823219154
5	15.424721844780834	35.864513936693434	32.85525969376299	15.855504524762743
6	34.95996942147008	34.953099395898825	16.643951011645846	13.442980170985253
7	31.199090208467766	29.9681605238909	20.759633510593297	18.073115757048036
8	28.950182193916298	31.633894200732833	19.280856198443132	20.13506740690774
9	27.85843518482657	14.14419386548779	18.3662817611594	39.63108918852624
10	16.552807825195075	25.4776227205274	30.400760221878926	27.5688092323986
11	38.1537516673898	20.66387588764156	22.065682132451496	19.116690312517147
12	25.787254899248524	23.132263802781445	27.610081510437563	23.47039978753247
13	29.29164184225137	18.60129560391116	25.210531024216948	26.89653152962052
14	24.465534262846983	19.66353893024313	24.015279474293123	31.85564733261676
15	25.833071230726233	26.46921962292274	21.696077499974635	26.00163164637639
16	27.05697442892307	24.926220429378656	22.659458402616963	25.357346739081315
17	24.934289879915173	25.202802678717806	24.35875707973366	25.50415036163336
18	26.2404509536078	23.709485357264306	25.336238249211814	24.71382543991608
19	26.427730402908107	24.368658486172908	24.536040214036504	24.66757089688248
20	26.53007539425093	23.40822959764987	23.897063400612247	26.164631607486953
21	28.18294808768933	23.438477316966726	23.412909292996876	24.96566530234707
22	26.76299945013078	23.288171817415755	24.01745748312063	25.931371249332834
23	24.825317835541235	23.1376803942355	24.87581000741013	27.161191762813136
24	25.597868629261615	24.476570254650845	24.128873686771044	25.796687429316496
25	25.663338101601934	23.669980475000717	24.351510858648044	26.31517056474931
26	25.487123072634343	24.69596672125965	24.85877725393209	24.958132952173916
27	26.36570613060785	23.926420255756124	23.72966349152187	25.978210122114152
28	25.231748534856806	23.74172903035417	25.05549330714289	25.971029127646133
29	25.151495099796666	24.06816363015529	24.99914116463609	25.781200105411955
30	25.090966600969526	24.31216846440447	25.264473447297007	25.332391487328998
31	26.080359804466525	24.117278586788096	23.55542993180121	26.24693167694417
32	25.514832990810675	24.026807604325697	23.46518418988589	26.993175214977732
33	25.077195592986424	23.642566824650668	24.442869497313918	26.83736808504899
34	26.21752569285516	23.69313091078618	24.567935335209505	25.52140806114916
35	27.022101504829916	23.5151664674138	24.524657974860705	24.938074052895583
36	25.397663132498117	24.406883143713017	23.950517028937387	26.24493669485148
37	26.725466417050157	24.12466684496417	23.93125247848902	25.218614259496647
38	25.4570831841481	23.846620444413293	24.59249664952833	26.103799721910274
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	194.0
1	302.5
2	411.0
3	411.0
4	939.5
5	1468.0
6	1468.0
7	1745.5
8	2023.0
9	2036.5
10	2050.0
11	2050.0
12	2662.0
13	3274.0
14	4554.5
15	5835.0
16	5835.0
17	8886.0
18	11937.0
19	11937.0
20	14722.5
21	17508.0
22	20073.5
23	22639.0
24	22639.0
25	27008.0
26	31377.0
27	31377.0
28	40179.0
29	48981.0
30	61266.0
31	73551.0
32	73551.0
33	102248.0
34	130945.0
35	130945.0
36	146826.5
37	162708.0
38	188631.5
39	214555.0
40	214555.0
41	229375.0
42	244195.0
43	286618.0
44	329041.0
45	329041.0
46	335153.5
47	341266.0
48	341266.0
49	373418.0
50	405570.0
51	414051.5
52	422533.0
53	422533.0
54	407563.5
55	392594.0
56	392594.0
57	385314.5
58	378035.0
59	340442.5
60	302850.0
61	302850.0
62	292540.5
63	282231.0
64	233670.0
65	185109.0
66	185109.0
67	154916.5
68	124724.0
69	124724.0
70	100819.0
71	76914.0
72	59602.0
73	42290.0
74	42290.0
75	31724.0
76	21158.0
77	21158.0
78	21414.0
79	21670.0
80	17045.0
81	12420.0
82	12420.0
83	12620.0
84	12820.0
85	12820.0
86	8567.5
87	4315.0
88	3752.0
89	3189.0
90	3189.0
91	2296.5
92	1404.0
93	1225.5
94	1047.0
95	1047.0
96	948.5
97	850.0
98	850.0
99	1434.5
100	2019.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12068607787537175
2	0.05562855891371003
3	0.012310671554049381
4	0.002904765198146483
5	3.2275168868294257E-4
6	3.6885907278050577E-4
7	6.916107614634484E-5
8	3.2275168868294257E-4
9	2.7664430458537937E-4
10	4.380201489268507E-4
11	0.0018904027480000922
12	0.002213154436683035
13	0.013186711851903083
14	0.00871429559443945
15	0.022500403439610853
16	0.010512483574244415
17	0.01957258454941559
18	0.0051870807109758625
19	0.007123590843073518
20	0.005394563939414897
21	0.005786476704244184
22	0.0070313760748783915
23	0.009083154667219956
24	0.013486409848537242
25	0.016045369665952002
26	0.01745164488092768
27	0.008414597597805287
28	0.005532886091707588
29	0.009936141273024876
30	0.002743389353805012
31	0.005371510247366116
32	0.006316711621366162
33	0.007861308988634531
34	0.011572953408488369
35	0.015330705212439772
36	0.014108859533854348
37	0.008760402978537012
38	0.005786476704244184
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	4337700.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.28883930811954
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.48951454267008	27.372505345397702
2	14.449068208298726	11.064761015592174
3	5.644841988364354	6.484033434366264
4	2.6792565175072016	4.103424890562608
5	1.541379397899504	2.950881403951008
6	0.9373518938312555	2.1534069622839884
7	0.6091935680321762	1.6327720243746786
8	0.4320943533760292	1.3235513009888482
9	0.3316654795724012	1.142917762625827
>10	1.6552263423791138	11.201759170957068
>50	0.10286732887303066	2.7236422203336113
>100	0.0905424405549788	7.452511303417494
>500	0.018317606897811426	5.00532557232257
>1k	0.017531702930782968	12.731071114335249
>5k	0.0011486288127064703	2.657436478490873
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	7154	0.16492611291698364	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	7123	0.1642114484634714	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	7022	0.1618830255665445	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	6894	0.15893215298430044	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	6779	0.15628097839869054	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	6615	0.15250017290269038	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	6394	0.14740530695990964	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	6278	0.14473107868225096	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	6193	0.14277151485810452	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	6168	0.142195172556885	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	5936	0.13684671600156764	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	5545	0.12783272241049404	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	5513	0.12709500426493303	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	5440	0.12541208474537197	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	5349	0.12331419876893285	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	5205	0.11999446711390829	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	5163	0.11902621204785946	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	5030	0.1159600710053715	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	5005	0.11538372870415196	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	4942	0.11393134610507873	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	4888	0.11268644673444453	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	4723	0.10888258754639556	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	4680	0.10789127878829793	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4636	0.10687691633815155	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	4426	0.10203564100790742	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	4405	0.101551513474883	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	4370	0.10074463425317563	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	4370	0.10074463425317563	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1526846024390806E-4
2	0.0	0.0	0.0	0.0	1.1526846024390806E-4
3	0.0	0.0	0.0	0.0	1.1526846024390806E-4
4	0.0	0.0	0.0	0.0	1.1526846024390806E-4
5	0.0	0.0	0.0	2.3053692048781614E-5	1.3832215229268968E-4
6	0.0	0.0	0.0	2.3053692048781614E-5	1.6137584434147128E-4
7	0.0	0.0	0.0	2.3053692048781614E-5	1.6137584434147128E-4
8	0.0	0.0	0.0	2.3053692048781614E-5	1.6137584434147128E-4
9	0.0	0.0	0.0	2.3053692048781614E-5	1.6137584434147128E-4
10	0.0	0.0	0.0	4.610738409756323E-5	1.844295363902529E-4
11	0.0	0.0	0.0	4.610738409756323E-5	1.844295363902529E-4
12	0.0	0.0	0.0	4.610738409756323E-5	2.7664430458537937E-4
13	4.610738409756323E-5	0.0	0.0	4.610738409756323E-5	4.610738409756322E-4
14	4.610738409756323E-5	0.0	0.0	9.221476819512646E-5	4.610738409756322E-4
15	4.610738409756323E-5	0.0	0.0	9.221476819512646E-5	5.302349171219771E-4
16	4.610738409756323E-5	0.0	0.0	1.1526846024390806E-4	5.302349171219771E-4
17	4.610738409756323E-5	0.0	0.0	1.3832215229268968E-4	5.302349171219771E-4
18	4.610738409756323E-5	0.0	0.0	1.3832215229268968E-4	5.302349171219771E-4
19	4.610738409756323E-5	0.0	0.0	2.305369204878161E-4	5.532886091707587E-4
20	4.610738409756323E-5	0.0	0.0	2.7664430458537937E-4	7.607718376097932E-4
21	4.610738409756323E-5	0.0	0.0	4.14966456878069E-4	7.838255296585749E-4
22	4.610738409756323E-5	0.0	2.3053692048781614E-5	5.763423012195403E-4	8.068792217073564E-4
23	4.610738409756323E-5	0.0	2.3053692048781614E-5	0.0010374161421951725	8.068792217073564E-4
24	4.610738409756323E-5	0.0	2.3053692048781614E-5	0.0014754362911220233	8.29932913756138E-4
25	6.916107614634484E-5	0.0	2.3053692048781614E-5	0.0017290269036586208	8.29932913756138E-4
26	6.916107614634484E-5	0.0	2.3053692048781614E-5	0.002259261820780598	8.760402978537013E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	1245	0.0	16.83423	28
GTATCAA	7480	0.0	15.7172575	1
CTAGCGG	1330	0.0	15.6382475	29
ATACCGT	1340	0.0	15.519754	6
ACCGTCG	1285	0.0	15.4383135	8
TACCGTC	1350	0.0	15.050513	7
CTATTCC	1635	0.0	14.480556	4
GTTCAAA	2225	0.0	14.39734	1
TCTATAC	280	0.0	14.284608	3
TAGCGGC	1445	0.0	14.28296	30
GTCCTAT	1690	0.0	14.216308	1
GTATTAC	205	3.6379788E-12	14.063744	1
ATCGTTT	1280	0.0	13.999239	29
AGGCCCG	2070	0.0	13.913409	10
CGTCGTA	1415	0.0	13.908493	10
CCGTCGT	1445	0.0	13.841194	9
CATCGTT	1305	0.0	13.730895	28
CCTATTC	1690	0.0	13.7267475	3
GTATAAT	375	0.0	13.667875	1
CGAGCCG	2085	0.0	13.276435	15
>>END_MODULE