FastQCFastQC Report
Thu 2 Feb 2017
SRR4062740_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062740_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4337700
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG77670.1790580261428868No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT74950.1727874219056182No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA72810.16785393180717892No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA71680.1652488646056666No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC68500.15791779053415403No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC68030.15683426700786132No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG66460.15321483735620262No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA65200.1503100721580561No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG64190.14798164926112917No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC63070.14539963575166562No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT61930.14277151485810452No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC57550.13267399774073818No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG56760.13085275606888444No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG56620.1305300043802015No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG55150.1271411116490306No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT54390.1253890310533232No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG53380.12306060815639623No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT52140.12020195034234732No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT51900.11964866173317656No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC51870.11957950065703021No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG48560.11194872858888351No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT48540.11190262120478595No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC48060.11079604398644442No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT47960.11056550706595662No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT47190.10879037277820042No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT47020.10839846001337114No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA46370.10689997003020034No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT45870.10574728542776124No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA43810.10099822486571225No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTT600.002442743616.00061610
GTATCAA73800.015.1987691
AACCGCG10550.015.167167
CTAGCGG14700.014.80226829
TCTAGCG14650.014.74357628
ACCGTCG13800.014.6099338
AGAACCG12250.014.3685635
AAGACGG20300.014.1883945
TAGTACT2600.014.1542284
TCTAGAT11050.014.0451432
TACCGTC14750.013.9944057
CAAGACG20400.013.8827294
CGCGGTC11650.013.871778510
TAGCGGC16000.013.79957830
GAACCGC12650.013.7877286
ATCGTTT13350.013.66250329
CGTCGTA14650.013.65240310
CATCGTT13600.013.64664128
ACGAACG6950.013.58247515
TAGGATA1657.563358E-913.5761254
Produced by FastQC (version 0.11.5)