##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062740_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4337700
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21359084307352	32.0	32.0	32.0	32.0	32.0
2	31.270929755400328	32.0	32.0	32.0	32.0	32.0
3	31.36166862623049	32.0	32.0	32.0	32.0	32.0
4	31.453830832007746	32.0	32.0	32.0	32.0	32.0
5	31.38582244046384	32.0	32.0	32.0	32.0	32.0
6	34.89735712474353	36.0	36.0	36.0	36.0	36.0
7	34.920224081886715	36.0	36.0	36.0	36.0	36.0
8	34.85110242755377	36.0	36.0	36.0	32.0	36.0
9	34.97628766396939	36.0	36.0	36.0	36.0	36.0
10	34.81386011019665	36.0	36.0	36.0	32.0	36.0
11	34.99436221960947	36.0	36.0	36.0	36.0	36.0
12	34.878446411692835	36.0	36.0	36.0	32.0	36.0
13	34.94465454042465	36.0	36.0	36.0	36.0	36.0
14	34.873134841044795	36.0	36.0	36.0	32.0	36.0
15	34.847062729096066	36.0	36.0	36.0	32.0	36.0
16	34.85997118288494	36.0	36.0	36.0	32.0	36.0
17	34.82247181686147	36.0	36.0	36.0	32.0	36.0
18	34.81928694930493	36.0	36.0	36.0	32.0	36.0
19	34.81938838554995	36.0	36.0	36.0	32.0	36.0
20	34.80816146805911	36.0	36.0	36.0	32.0	36.0
21	34.80121008829564	36.0	36.0	36.0	32.0	36.0
22	34.786538257601954	36.0	36.0	36.0	32.0	36.0
23	34.71735850796505	36.0	36.0	36.0	32.0	36.0
24	34.69316458030754	36.0	36.0	36.0	32.0	36.0
25	34.673926043755905	36.0	36.0	36.0	32.0	36.0
26	34.60458468773774	36.0	36.0	36.0	32.0	36.0
27	34.59128224635175	36.0	36.0	36.0	32.0	36.0
28	34.56167185374738	36.0	36.0	36.0	32.0	36.0
29	34.515877538787834	36.0	36.0	36.0	32.0	36.0
30	34.50373769509187	36.0	36.0	36.0	32.0	36.0
31	34.50545819212947	36.0	36.0	36.0	32.0	36.0
32	34.463852963552114	36.0	36.0	36.0	32.0	36.0
33	34.4179869516103	36.0	36.0	36.0	32.0	36.0
34	34.41571132166816	36.0	36.0	36.0	32.0	36.0
35	34.38564377435046	36.0	36.0	36.0	32.0	36.0
36	34.35847292343869	36.0	36.0	36.0	32.0	36.0
37	34.359326832192174	36.0	36.0	36.0	32.0	36.0
38	33.920145468796825	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	8.0
20	22.0
21	102.0
22	390.0
23	1288.0
24	3360.0
25	7943.0
26	16584.0
27	31518.0
28	53924.0
29	83436.0
30	124449.0
31	177352.0
32	253426.0
33	403877.0
34	983941.0
35	2196080.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.65275152639706	18.365043259252563	12.092624554507857	26.889580659842512
2	16.15017175000576	20.505659681397976	35.83447449108974	27.509694077506513
3	17.466053438458168	24.452728404453975	28.65260391451691	29.42861424257095
4	12.20408199022152	16.11918607331832	35.421473374756324	36.25525856170384
5	14.807465805011278	36.42589604575884	32.57166187495346	16.194976274276417
6	34.94618797277446	35.38351731750433	16.283382960339278	13.38691174938193
7	30.892295576641597	30.171448911479576	20.77187858847028	18.164376923408543
8	28.768857717630013	31.897121320615433	19.108131797804045	20.2258891639505
9	27.827373198922338	13.939023529916254	18.16307484745969	40.07052842370172
10	16.315456284554873	25.720306727166175	30.38747105009929	27.57676593817966
11	38.27853078780057	20.603894287951924	21.98315105011411	19.134423874133397
12	25.633195041409195	23.22896273679687	27.492551324623925	23.64529089717001
13	29.294979910975698	18.623129535245933	25.167531489096046	26.914359064682326
14	24.38467390552597	19.627106531110957	23.990294395647464	31.997925167715607
15	25.784286603499552	26.618830255665443	21.55418770316066	26.042695437674347
16	26.987471234742483	24.977810811172194	22.534441079116156	25.500276874969163
17	24.908914862715264	25.337644373746453	24.203886852479425	25.549553911058858
18	26.253821149457085	23.717868916707012	25.263204002120936	24.765105931714963
19	26.410125181547823	24.36346450884109	24.429121423796023	24.797288885815064
20	26.47239320377158	23.406644074048458	23.88138875440902	26.23957396777094
21	28.19078879875658	23.549247446283132	23.299760218383163	24.960203536577126
22	26.660217779057515	23.454070925622087	24.021583793619612	25.864127501700786
23	24.721344068061924	23.04859006979971	24.93115586450598	27.298909997632386
24	25.539156695944854	24.468197431818705	24.139451783203082	25.85319408903336
25	25.66363541214718	23.66013032719198	24.376685513837064	26.299548746823774
26	25.605926649983456	24.58914239014039	24.769399416049307	25.03553154382685
27	26.45758187868312	23.852882335284754	23.640534679915476	26.049001106116656
28	25.24213292758835	23.735966064965304	25.061783894690738	25.96011711275561
29	25.258385780482744	23.956612951564193	24.955414159577657	25.829587108375407
30	25.167323696890058	24.158240542222835	25.295525278373333	25.378910482513774
31	26.089794130530002	24.02365308804205	23.549715286903197	26.33683749452475
32	25.5998109597252	24.021624363141758	23.519099983862414	26.85946469327063
33	25.075961915300738	23.585517670654955	24.49477833875095	26.84374207529336
34	26.072757452105954	23.585125757890125	24.73776425294511	25.604352537058812
35	27.042995135670978	23.465753740461533	24.5288747492911	24.96237637457639
36	25.46019780067778	24.357078636143576	23.959356340918	26.223367222260645
37	26.793162274938332	24.052377988334833	23.861608686631165	25.29285105009567
38	25.493263127075554	23.739036515937613	24.655145186556453	26.11255517043038
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	114.0
1	160.0
2	206.0
3	206.0
4	699.0
5	1192.0
6	1192.0
7	1470.0
8	1748.0
9	1920.5
10	2093.0
11	2093.0
12	2700.0
13	3307.0
14	4575.0
15	5843.0
16	5843.0
17	9114.5
18	12386.0
19	12386.0
20	14981.0
21	17576.0
22	20079.0
23	22582.0
24	22582.0
25	26994.5
26	31407.0
27	31407.0
28	40324.5
29	49242.0
30	61331.5
31	73421.0
32	73421.0
33	103269.5
34	133118.0
35	133118.0
36	148376.0
37	163634.0
38	189618.0
39	215602.0
40	215602.0
41	230223.5
42	244845.0
43	287563.0
44	330281.0
45	330281.0
46	335873.0
47	341465.0
48	341465.0
49	374029.0
50	406593.0
51	414162.0
52	421731.0
53	421731.0
54	406954.5
55	392178.0
56	392178.0
57	385902.0
58	379626.0
59	341972.0
60	304318.0
61	304318.0
62	294005.0
63	283692.0
64	234356.5
65	185021.0
66	185021.0
67	154989.0
68	124957.0
69	124957.0
70	101175.5
71	77394.0
72	59702.0
73	42010.0
74	42010.0
75	30995.5
76	19981.0
77	19981.0
78	20985.5
79	21990.0
80	16842.0
81	11694.0
82	11694.0
83	11363.5
84	11033.0
85	11033.0
86	6887.5
87	2742.0
88	2395.0
89	2048.0
90	2048.0
91	1225.0
92	402.0
93	261.5
94	121.0
95	121.0
96	83.5
97	46.0
98	46.0
99	53.5
100	61.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008345436521658943
2	0.0
3	0.0
4	2.7664430458537937E-4
5	6.916107614634484E-5
6	1.8442953639025289E-4
7	5.302349171219771E-4
8	0.0019826175161952184
9	0.0036424833437074945
10	7.838255296585749E-4
11	0.005048758558683173
12	3.6885907278050577E-4
13	1.8442953639025289E-4
14	0.0
15	0.0
16	4.610738409756322E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	6.916107614634484E-5
22	2.305369204878161E-5
23	6.916107614634484E-5
24	0.0
25	4.610738409756322E-5
26	1.1526846024390806E-4
27	4.610738409756322E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.1526846024390806E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	4337700.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.86311758933679
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.61432643415164	24.6072365722112
2	15.968252366470784	11.453426246298942
3	6.105977636192887	6.569381818939386
4	2.991325618763694	4.2911304965487185
5	1.7002984499510627	3.048900162378102
6	0.9955023630167892	2.142109098320025
7	0.6905732106112163	1.7336275779337125
8	0.4822515482353925	1.3836035185604483
9	0.37007647185917636	1.1944886424599277
>10	1.8276908494717292	11.610541077096382
>50	0.11452373395569573	2.839893457724546
>100	0.09833206780811067	7.608995686356003
>500	0.020111292099304994	5.14147159857171
>1k	0.019464626732782105	13.489599730809804
>5k	0.0012933306799190766	2.8855943157911192
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	7767	0.1790580261428868	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	7495	0.1727874219056182	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	7281	0.16785393180717892	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	7168	0.1652488646056666	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	6850	0.15791779053415403	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	6803	0.15683426700786132	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	6646	0.15321483735620262	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	6520	0.1503100721580561	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	6419	0.14798164926112917	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	6307	0.14539963575166562	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	6193	0.14277151485810452	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	5755	0.13267399774073818	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	5676	0.13085275606888444	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	5662	0.1305300043802015	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	5515	0.1271411116490306	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	5439	0.1253890310533232	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	5338	0.12306060815639623	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	5214	0.12020195034234732	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	5190	0.11964866173317656	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	5187	0.11957950065703021	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	4856	0.11194872858888351	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	4854	0.11190262120478595	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	4806	0.11079604398644442	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	4796	0.11056550706595662	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	4719	0.10879037277820042	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	4702	0.10839846001337114	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	4637	0.10689997003020034	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	4587	0.10574728542776124	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA	4381	0.10099822486571225	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.3053692048781614E-5
2	0.0	0.0	0.0	0.0	2.3053692048781614E-5
3	0.0	0.0	0.0	0.0	2.3053692048781614E-5
4	0.0	0.0	0.0	0.0	2.3053692048781614E-5
5	0.0	0.0	0.0	2.3053692048781614E-5	4.610738409756323E-5
6	0.0	0.0	0.0	2.3053692048781614E-5	4.610738409756323E-5
7	0.0	0.0	0.0	2.3053692048781614E-5	4.610738409756323E-5
8	2.3053692048781614E-5	0.0	0.0	2.3053692048781614E-5	4.610738409756323E-5
9	2.3053692048781614E-5	0.0	0.0	2.3053692048781614E-5	4.610738409756323E-5
10	2.3053692048781614E-5	0.0	0.0	4.610738409756323E-5	9.221476819512646E-5
11	2.3053692048781614E-5	0.0	0.0	4.610738409756323E-5	9.221476819512646E-5
12	2.3053692048781614E-5	0.0	0.0	4.610738409756323E-5	3.2275168868294257E-4
13	2.3053692048781614E-5	0.0	0.0	4.610738409756323E-5	3.919127648292874E-4
14	2.3053692048781614E-5	0.0	0.0	9.221476819512646E-5	3.919127648292874E-4
15	2.3053692048781614E-5	0.0	0.0	1.1526846024390806E-4	5.532886091707587E-4
16	2.3053692048781614E-5	0.0	0.0	1.3832215229268968E-4	5.993959932683219E-4
17	4.610738409756323E-5	0.0	0.0	1.6137584434147128E-4	5.993959932683219E-4
18	4.610738409756323E-5	0.0	0.0	2.074832284390345E-4	6.916107614634483E-4
19	4.610738409756323E-5	0.0	0.0	2.9969799663416097E-4	6.916107614634483E-4
20	4.610738409756323E-5	0.0	0.0	3.688590727805058E-4	8.29932913756138E-4
21	4.610738409756323E-5	0.0	0.0	4.610738409756322E-4	8.529866058049197E-4
22	4.610738409756323E-5	0.0	0.0	6.224496853171035E-4	8.760402978537013E-4
23	4.610738409756323E-5	0.0	0.0	0.0010143624501463909	8.990939899024829E-4
24	6.916107614634484E-5	0.0	0.0	0.00142932890702446	8.990939899024829E-4
25	9.221476819512646E-5	0.0	0.0	0.0016368121354634944	8.990939899024829E-4
26	9.221476819512646E-5	0.0	0.0	0.0020287249002927817	8.990939899024829E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTTT	60	0.0024427436	16.000616	10
GTATCAA	7380	0.0	15.198769	1
AACCGCG	1055	0.0	15.16716	7
CTAGCGG	1470	0.0	14.802268	29
TCTAGCG	1465	0.0	14.743576	28
ACCGTCG	1380	0.0	14.609933	8
AGAACCG	1225	0.0	14.368563	5
AAGACGG	2030	0.0	14.188394	5
TAGTACT	260	0.0	14.154228	4
TCTAGAT	1105	0.0	14.045143	2
TACCGTC	1475	0.0	13.994405	7
CAAGACG	2040	0.0	13.882729	4
CGCGGTC	1165	0.0	13.8717785	10
TAGCGGC	1600	0.0	13.799578	30
GAACCGC	1265	0.0	13.787728	6
ATCGTTT	1335	0.0	13.662503	29
CGTCGTA	1465	0.0	13.652403	10
CATCGTT	1360	0.0	13.646641	28
ACGAACG	695	0.0	13.582475	15
TAGGATA	165	7.563358E-9	13.576125	4
>>END_MODULE