FastQCFastQC Report
Thu 2 Feb 2017
SRR4062739_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062739_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3283794
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT47390.14431477735814122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11950.021.1710661
GTATCAA35850.021.1264021
TAGACCG450.00886968717.7722025
GTCCTAA11900.015.7431791
CTAGTAC1357.948984E-915.4039833
TAGGACG17350.015.3037914
ACGCTAT2950.015.18722810
GCGTGTA650.00416317514.7695479
GTAGGAC18300.014.685263
TGTAGGA20500.014.6766152
TAGGACC11550.014.5411154
GTATAGA3950.014.1881081
TTAGGAC13200.014.1786663
TATACGC3300.014.0585527
GTCCTAC17200.014.0573051
AGGACGT18950.013.927035
TATCAAC52500.013.9004042
GGACGTG18900.013.7950346
TACGTAC700.006817355313.7112383
TATACCG1402.067045E-713.7099865
Produced by FastQC (version 0.11.5)