##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062739_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3283794
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.33561240443219	32.0	32.0	32.0	32.0	32.0
2	31.0423671521417	32.0	32.0	32.0	32.0	32.0
3	31.06764096651617	32.0	32.0	32.0	32.0	32.0
4	31.12987020501286	32.0	32.0	32.0	32.0	32.0
5	31.094092077639463	32.0	32.0	32.0	32.0	32.0
6	34.74314619004724	36.0	36.0	36.0	32.0	36.0
7	34.69323623832676	36.0	36.0	36.0	32.0	36.0
8	34.69566209086197	36.0	36.0	36.0	32.0	36.0
9	34.7947715356079	36.0	36.0	36.0	32.0	36.0
10	34.587731142696526	36.0	36.0	36.0	32.0	36.0
11	34.75654319363517	36.0	36.0	36.0	32.0	36.0
12	34.63876022673773	36.0	36.0	36.0	32.0	36.0
13	34.677276650118735	36.0	36.0	36.0	32.0	36.0
14	34.609426474376896	36.0	36.0	36.0	32.0	36.0
15	34.572484753915745	36.0	36.0	36.0	32.0	36.0
16	34.591742661080445	36.0	36.0	36.0	32.0	36.0
17	34.54708882469485	36.0	36.0	36.0	32.0	36.0
18	34.55621241770952	36.0	36.0	36.0	32.0	36.0
19	34.53604215124335	36.0	36.0	36.0	32.0	36.0
20	34.52590083299988	36.0	36.0	36.0	32.0	36.0
21	34.52238812787891	36.0	36.0	36.0	32.0	36.0
22	34.495374557600144	36.0	36.0	36.0	32.0	36.0
23	34.44637574707792	36.0	36.0	36.0	32.0	36.0
24	34.4382375995571	36.0	36.0	36.0	32.0	36.0
25	34.41630412869991	36.0	36.0	36.0	32.0	36.0
26	34.38558326131298	36.0	36.0	36.0	32.0	36.0
27	34.38613293038479	36.0	36.0	36.0	32.0	36.0
28	34.36455270945741	36.0	36.0	36.0	32.0	36.0
29	34.33211553465291	36.0	36.0	36.0	32.0	36.0
30	34.311641046910985	36.0	36.0	36.0	32.0	36.0
31	34.31357478575087	36.0	36.0	36.0	32.0	36.0
32	34.285871160005776	36.0	36.0	36.0	32.0	36.0
33	34.27578343830338	36.0	36.0	36.0	32.0	36.0
34	34.271702183510904	36.0	36.0	36.0	32.0	36.0
35	34.249813477946546	36.0	36.0	36.0	32.0	36.0
36	34.21269056463347	36.0	36.0	36.0	32.0	36.0
37	34.20362757225332	36.0	36.0	36.0	32.0	36.0
38	33.68074611257588	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	6.0
8	7.0
9	10.0
10	26.0
11	20.0
12	20.0
13	22.0
14	201.0
15	627.0
16	881.0
17	961.0
18	1182.0
19	1505.0
20	1970.0
21	2893.0
22	4247.0
23	6907.0
24	10829.0
25	17182.0
26	25838.0
27	37710.0
28	53601.0
29	73060.0
30	99161.0
31	133684.0
32	182667.0
33	277678.0
34	659297.0
35	1691601.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.11409685518675	17.55781142096654	12.103132922439933	26.22495880140677
2	16.030849745214155	19.75522643093847	38.72022674172723	25.493697082120143
3	18.582798653480857	23.535700025796203	29.339936998789984	28.541564321932956
4	12.719417388211331	16.151221063094756	36.70498121628798	34.424380332405924
5	14.239078234976873	36.70798380041093	34.478850181208465	14.574087783403735
6	33.145166324835515	36.203588237354154	17.45187337173414	13.19937206607619
7	29.695355340607644	30.958041508706547	21.110130876093592	18.236472274592217
8	27.107150502941273	34.442592583005634	19.632813739519328	18.817443174533764
9	27.3676094095019	14.89980927508392	18.709678794635582	39.0229025207786
10	15.36026023675771	27.600913459766936	32.85394662673706	24.184879676738294
11	36.04116533190569	21.566606795800148	23.5054361894112	18.88679168288296
12	24.543573917256623	23.798869513499785	29.704368735570856	21.953187833672736
13	29.5621756292488	20.552039423990642	25.14409371116688	24.741691235593674
14	22.94339149142491	20.13784832116326	25.962550525908007	30.956209661503824
15	24.4769590903345	28.463629676222563	22.561525937681335	24.497885295761606
16	24.553693253805818	26.588875163245813	24.83727194316122	24.02015963978715
17	23.45018345656164	26.664587309545407	25.94599072939746	23.939238504495496
18	23.624097753417047	25.968378086428682	27.173850479761754	23.23367368039252
19	25.046961511052096	25.533174561601196	25.922053637232988	23.49781029011372
20	25.40700055883871	25.346061252986434	25.970498797811548	23.276439390363308
21	25.591873789627655	24.991739242056806	25.23924220907742	24.177144759238118
22	25.26654094899785	25.62204054882097	25.455696094590362	23.65572240759081
23	24.09516511789959	25.692894594502402	25.983166599258347	24.22877368833966
24	24.519369876715274	25.445056093057765	26.107591948817255	23.927982081409706
25	24.386813256432184	25.516298852087125	25.963000460529685	24.133887430951006
26	23.957729233168447	26.112326080070176	26.07976558883014	23.85017909793124
27	24.365295791256763	26.038755010662413	25.604553436609372	23.991395761471455
28	23.978059487039555	25.85821806879388	25.754399279328883	24.40932316483768
29	24.20309051310485	26.011640971272794	25.589883422778136	24.195385092844223
30	24.02457593568231	25.82854706580991	26.000822243201267	24.146054755306515
31	24.680085820048937	25.532804546208983	25.48298140920906	24.304128224533024
32	24.211901264691722	25.915658185138295	25.454452429324576	24.41798812084541
33	23.630483570492945	25.81083126517239	26.194840708716548	24.363844455618114
34	24.210035165046687	25.741630408232506	26.152366437694884	23.895967989025923
35	24.304648590169688	25.65590856814261	26.206341799787523	23.833101041900175
36	23.90017982105612	25.92688251691	26.297184752733866	23.87575290930001
37	24.41694234080694	25.63187802157842	25.46403708763849	24.487142549976156
38	23.919858642866757	26.11656481700866	25.84722612207706	24.11635041804752
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	31.0
1	98.0
2	165.0
3	165.0
4	428.5
5	692.0
6	692.0
7	804.5
8	917.0
9	954.5
10	992.0
11	992.0
12	1577.0
13	2162.0
14	3388.0
15	4614.0
16	4614.0
17	7160.0
18	9706.0
19	9706.0
20	13379.5
21	17053.0
22	21063.0
23	25073.0
24	25073.0
25	32618.5
26	40164.0
27	40164.0
28	52609.0
29	65054.0
30	82087.0
31	99120.0
32	99120.0
33	122596.5
34	146073.0
35	146073.0
36	162542.0
37	179011.0
38	197967.0
39	216923.0
40	216923.0
41	229747.0
42	242571.0
43	257494.5
44	272418.0
45	272418.0
46	281532.0
47	290646.0
48	290646.0
49	297432.5
50	304219.0
51	298737.0
52	293255.0
53	293255.0
54	281317.5
55	269380.0
56	269380.0
57	250460.0
58	231540.0
59	207087.5
60	182635.0
61	182635.0
62	161168.5
63	139702.0
64	116064.5
65	92427.0
66	92427.0
67	76272.5
68	60118.0
69	60118.0
70	48882.5
71	37647.0
72	29993.5
73	22340.0
74	22340.0
75	18111.5
76	13883.0
77	13883.0
78	11809.0
79	9735.0
80	7828.0
81	5921.0
82	5921.0
83	4560.0
84	3199.0
85	3199.0
86	2256.0
87	1313.0
88	1053.0
89	793.0
90	793.0
91	610.5
92	428.0
93	358.0
94	288.0
95	288.0
96	274.0
97	260.0
98	260.0
99	793.0
100	1326.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12025723903509172
2	0.05548460104379264
3	0.011115191756851982
4	0.002984352855264368
5	2.1316806109031202E-4
6	2.740732214018297E-4
7	3.0452580155758855E-5
8	3.3497838171334744E-4
9	2.1316806109031202E-4
10	4.5678870233638286E-4
11	0.0018271548093455315
12	0.002283943511681914
13	0.015652626200060055
14	0.010049351451400422
15	0.02524518894912409
16	0.012241937222615061
17	0.02216947835339245
18	0.005633727328815389
19	0.007734955359562749
20	0.006060063450996012
21	0.005999158290684495
22	0.007491334718316678
23	0.009805730810154352
24	0.015226290077879429
25	0.018819694536258973
26	0.02052503902498147
27	0.009013963726104622
28	0.005725085069282665
29	0.011906958840901713
30	0.0028625425346413325
31	0.005451011847880835
32	0.006912735695357261
33	0.00855717502376824
34	0.012790083665418719
35	0.01711435004753648
36	0.016139867482552195
37	0.009988446291088904
38	0.005968705710528736
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	3283794.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.769898150246487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.01050487430744	15.55406015703402
2	13.213814363297583	7.33892558090076
3	7.444293462611724	6.2018181377182
4	4.761104437387241	5.288615412357211
5	3.3734568133154648	4.684027606001276
6	2.6045774604042564	4.339731047991231
7	1.9573143467195706	3.80480940394931
8	1.5708347904779127	3.4897537713948332
9	1.2639917949911494	3.159083106868631
>10	7.485788182264039	36.38158493465613
>50	0.23389010147882414	4.27961916694416
>100	0.07337219345773108	3.7219857885884595
>500	0.005623689744686184	1.0884693398397027
>1k	0.0014334895424627965	0.667516545755889
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4739	0.14431477735814122	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.0452580155758858E-5	0.0
2	0.0	0.0	0.0	6.0905160311517716E-5	0.0
3	0.0	3.0452580155758858E-5	0.0	1.2181032062303543E-4	0.0
4	0.0	3.0452580155758858E-5	0.0	2.13168061090312E-4	0.0
5	0.0	3.0452580155758858E-5	0.0	2.13168061090312E-4	0.0
6	0.0	3.0452580155758858E-5	0.0	3.6543096186910626E-4	0.0
7	0.0	3.0452580155758858E-5	0.0	4.26336122180624E-4	0.0
8	0.0	3.0452580155758858E-5	0.0	4.26336122180624E-4	0.0
9	0.0	3.0452580155758858E-5	0.0	6.39504183270936E-4	0.0
10	0.0	3.0452580155758858E-5	0.0	6.39504183270936E-4	0.0
11	0.0	3.0452580155758858E-5	0.0	0.001035387725295801	0.0
12	0.0	3.0452580155758858E-5	0.0	0.0013094609466976308	0.0
13	0.0	3.0452580155758858E-5	0.0	0.0013094609466976308	0.0
14	0.0	3.0452580155758858E-5	0.0	0.0013094609466976308	0.0
15	0.0	3.0452580155758858E-5	0.0	0.0013094609466976308	0.0
16	0.0	3.0452580155758858E-5	0.0	0.0013703661070091485	0.0
17	0.0	3.0452580155758858E-5	0.0	0.0016139867482552195	0.0
18	0.0	3.0452580155758858E-5	0.0	0.0016444393284109782	0.0
19	0.0	3.0452580155758858E-5	0.0	0.0016444393284109782	0.0
20	0.0	3.0452580155758858E-5	0.0	0.001705344488722496	0.0
21	0.0	3.0452580155758858E-5	0.0	0.0017662496490340137	0.0
22	0.0	3.0452580155758858E-5	0.0	0.0024666589926164674	0.0
23	0.0	3.0452580155758858E-5	0.0	0.002771184794174056	0.0
24	0.0	3.0452580155758858E-5	0.0	0.00292344769495285	0.0
25	0.0	3.0452580155758858E-5	0.0	0.0031061631758874035	0.0
26	0.0	3.0452580155758858E-5	0.0	0.003410688977444992	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1195	0.0	21.171066	1
GTATCAA	3585	0.0	21.126402	1
TAGACCG	45	0.008869687	17.772202	5
GTCCTAA	1190	0.0	15.743179	1
CTAGTAC	135	7.948984E-9	15.403983	3
TAGGACG	1735	0.0	15.303791	4
ACGCTAT	295	0.0	15.187228	10
GCGTGTA	65	0.004163175	14.769547	9
GTAGGAC	1830	0.0	14.68526	3
TGTAGGA	2050	0.0	14.676615	2
TAGGACC	1155	0.0	14.541115	4
GTATAGA	395	0.0	14.188108	1
TTAGGAC	1320	0.0	14.178666	3
TATACGC	330	0.0	14.058552	7
GTCCTAC	1720	0.0	14.057305	1
AGGACGT	1895	0.0	13.92703	5
TATCAAC	5250	0.0	13.900404	2
GGACGTG	1890	0.0	13.795034	6
TACGTAC	70	0.0068173553	13.711238	3
TATACCG	140	2.067045E-7	13.709986	5
>>END_MODULE