FastQCFastQC Report
Thu 2 Feb 2017
SRR4062739_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062739_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3283794
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT42470.12933210792150784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33700.020.9879191
GGTATCA11600.020.9682711
ACGCGTA659.445517E-619.69159527
GTCGCGA904.3745058E-717.77713630
CGTAGAC703.6983137E-416.0003953
CGCGTAG600.002443792815.99942128
TAGGACG16850.015.6682824
TCGCGAA1052.1886572E-615.23754431
TTAGGAC12650.014.925273
TATACGG650.00416097614.77072055
AGGACGT17500.014.6300485
CGTGATT7800.014.56357628
ATACCCG1103.5433495E-614.5469235
GTCCTAA12900.014.5135341
GTAGGAC17350.014.4787443
GGACGTG17550.014.4060126
TCGCGTA1707.585186E-1014.118649
TAGGACC9800.013.8781064
TATCAAC50500.013.8773592
CGCGTAA1902.7830538E-1013.47442810
Produced by FastQC (version 0.11.5)