##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062739_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3283794
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2885549458949	32.0	32.0	32.0	32.0	32.0
2	31.38720577478368	32.0	32.0	32.0	32.0	32.0
3	31.42760416761831	32.0	32.0	32.0	32.0	32.0
4	31.503617766522503	32.0	32.0	32.0	32.0	32.0
5	31.45764655151937	32.0	32.0	32.0	32.0	32.0
6	34.97749249800688	36.0	36.0	36.0	36.0	36.0
7	34.999638832399356	36.0	36.0	36.0	36.0	36.0
8	34.938906338217315	36.0	36.0	36.0	36.0	36.0
9	35.02821644719492	36.0	36.0	36.0	36.0	36.0
10	34.91166071927776	36.0	36.0	36.0	32.0	36.0
11	35.04966907181145	36.0	36.0	36.0	36.0	36.0
12	34.98119553175382	36.0	36.0	36.0	36.0	36.0
13	35.01300507888132	36.0	36.0	36.0	36.0	36.0
14	34.969059569510144	36.0	36.0	36.0	36.0	36.0
15	34.94507664000848	36.0	36.0	36.0	36.0	36.0
16	34.96486320396468	36.0	36.0	36.0	36.0	36.0
17	34.936704007620456	36.0	36.0	36.0	36.0	36.0
18	34.936997265967356	36.0	36.0	36.0	36.0	36.0
19	34.92553978720955	36.0	36.0	36.0	36.0	36.0
20	34.912460708558456	36.0	36.0	36.0	32.0	36.0
21	34.90587229284176	36.0	36.0	36.0	32.0	36.0
22	34.89276824307493	36.0	36.0	36.0	32.0	36.0
23	34.84207444194124	36.0	36.0	36.0	32.0	36.0
24	34.82977708102274	36.0	36.0	36.0	32.0	36.0
25	34.8228469873567	36.0	36.0	36.0	32.0	36.0
26	34.75451840158061	36.0	36.0	36.0	32.0	36.0
27	34.73991699844753	36.0	36.0	36.0	32.0	36.0
28	34.71050833273951	36.0	36.0	36.0	32.0	36.0
29	34.6838397901939	36.0	36.0	36.0	32.0	36.0
30	34.66341006774481	36.0	36.0	36.0	32.0	36.0
31	34.64516805865411	36.0	36.0	36.0	32.0	36.0
32	34.630992991643204	36.0	36.0	36.0	32.0	36.0
33	34.61678290416512	36.0	36.0	36.0	32.0	36.0
34	34.61048165627929	36.0	36.0	36.0	32.0	36.0
35	34.59741658581507	36.0	36.0	36.0	32.0	36.0
36	34.57016761709169	36.0	36.0	36.0	32.0	36.0
37	34.55447570706323	36.0	36.0	36.0	32.0	36.0
38	34.18326667263537	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	6.0
21	59.0
22	228.0
23	771.0
24	2029.0
25	5178.0
26	10892.0
27	21190.0
28	36574.0
29	57748.0
30	86164.0
31	122233.0
32	171572.0
33	268957.0
34	670657.0
35	1829534.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.669232537989366	18.695928259151483	12.634898590978793	26.999940611880362
2	15.178940938116378	20.750181269038578	37.95613791734132	26.114739875503723
3	17.684574610953064	24.260261149146384	29.527491675787214	28.527672564113338
4	12.291441380673362	17.037909561180697	36.469151946120775	34.201497112025166
5	13.793378571670017	37.31855205245885	34.10020059120657	14.787868784664562
6	33.18221724964667	36.56562586694821	17.16952581301661	13.082631070388507
7	29.624828246506464	31.15093721374418	20.99774771482586	18.2264868249235
8	27.085722012145375	34.64510818373923	19.442102079989183	18.827067724126213
9	27.325838481019442	14.783523100330239	18.51577317615989	39.37486524249043
10	15.169430671284948	27.876563526832943	32.770036318101006	24.183969483781105
11	36.19407962256543	21.547994574317176	23.385618773301108	18.872307029816284
12	24.43331365074976	23.88766249170545	29.634662217326785	22.044361640218003
13	29.56047258994749	20.537387282997596	25.08026580367668	24.821874323378232
14	22.920987126476263	20.168134785555974	25.81754519315158	31.09333289481618
15	24.413315817009227	28.645371786415346	22.427624875372814	24.51368752120261
16	24.551486649737058	26.771084535474678	24.65064028091905	24.02678853386922
17	23.547183532219133	26.782130669585243	25.815657133181922	23.8550286650137
18	23.748870970590723	26.005559423033237	26.98354403473543	23.262025571640606
19	25.036923753438856	25.670428778419108	25.830974781000272	23.461672687141764
20	25.474831856078673	25.487743750064713	25.704566120773713	23.332858273082905
21	25.58247535543566	25.13473011495569	25.126842879882282	24.15595164972637
22	25.328857613393296	25.732232735812744	25.316219784931565	23.62268986586239
23	24.042675131692505	25.858506004958908	25.836823715065478	24.261995148283113
24	24.616404074067983	25.56162170952258	25.89617375511375	23.925800461295683
25	24.377251780031067	25.626113233150345	25.8497571861303	24.14687780068829
26	24.06756455111513	26.123692936795955	25.942104146028694	23.866638366060226
27	24.429135584714256	26.13971895905709	25.446282833991923	23.98486262223673
28	24.02818203577935	25.911948191634433	25.61692359508544	24.442946177500783
29	24.260809295589187	25.98159324245065	25.455464015099604	24.30213344686055
30	24.064176985523453	25.87467423352378	25.882378736303192	24.17877004464957
31	24.691591494472554	25.51971895922826	25.394772022849182	24.39391752345001
32	24.327043657427964	25.895351535449546	25.365842071701213	24.411762735421284
33	23.703131195196775	25.936310255759036	26.020024398607223	24.340534150436966
34	24.214947709874615	25.80359791144024	26.100936904081074	23.880517474604073
35	24.450254796738164	25.70310439692624	26.019841683126288	23.826799123209312
36	24.03506431889455	25.941822172767232	26.102368175348396	23.920745332989828
37	24.507536100011144	25.677676492496182	25.274606141554557	24.54018126593812
38	24.020332584910197	26.009952515277302	25.73344300325873	24.23627189655377
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	57.5
2	105.0
3	105.0
4	279.5
5	454.0
6	454.0
7	607.0
8	760.0
9	762.0
10	764.0
11	764.0
12	1354.5
13	1945.0
14	3136.5
15	4328.0
16	4328.0
17	6787.0
18	9246.0
19	9246.0
20	12823.5
21	16401.0
22	20572.5
23	24744.0
24	24744.0
25	32138.0
26	39532.0
27	39532.0
28	52052.5
29	64573.0
30	81626.0
31	98679.0
32	98679.0
33	122596.5
34	146514.0
35	146514.0
36	163421.5
37	180329.0
38	199533.5
39	218738.0
40	218738.0
41	231960.0
42	245182.0
43	259025.0
44	272868.0
45	272868.0
46	281426.0
47	289984.0
48	289984.0
49	297813.0
50	305642.0
51	299594.5
52	293547.0
53	293547.0
54	281229.5
55	268912.0
56	268912.0
57	251117.0
58	233322.0
59	208268.5
60	183215.0
61	183215.0
62	161872.0
63	140529.0
64	116182.0
65	91835.0
66	91835.0
67	75921.5
68	60008.0
69	60008.0
70	48643.0
71	37278.0
72	29642.0
73	22006.0
74	22006.0
75	17670.0
76	13334.0
77	13334.0
78	11382.5
79	9431.0
80	7420.5
81	5410.0
82	5410.0
83	3989.0
84	2568.0
85	2568.0
86	1738.5
87	909.0
88	696.0
89	483.0
90	483.0
91	293.0
92	103.0
93	78.0
94	53.0
95	53.0
96	40.5
97	28.0
98	28.0
99	26.5
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009409847268129486
2	3.0452580155758855E-5
3	0.0
4	1.522629007787943E-4
5	3.0452580155758855E-5
6	1.522629007787943E-4
7	5.481464428036594E-4
8	0.002283943511681914
9	0.003593404458379545
10	9.744825649842834E-4
11	0.004628792183675346
12	3.3497838171334744E-4
13	5.176938626479007E-4
14	0.0
15	0.0
16	3.0452580155758855E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	2.1316806109031202E-4
22	6.090516031151771E-5
23	2.4362064124607084E-4
24	0.0
25	9.135774046727656E-5
26	3.0452580155758855E-5
27	6.090516031151771E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	3.0452580155758855E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	3283794.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.49453088314676
#Duplication Level	Percentage of deduplicated	Percentage of total
1	51.337093382571574	13.088151126929603
2	14.315734298693922	7.299458603859511
3	8.166760666472191	6.246231960799306
4	5.348253255009561	5.454048311229258
5	3.752744029404931	4.7837224277104315
6	2.8768819249754216	4.400685305007156
7	2.2400540085591354	3.99763882707893
8	1.757622201851144	3.5847802804798725
9	1.406669573978982	3.2276142806571055
>10	8.443797469424842	37.74726956687957
>50	0.2626093936344478	4.436957108028095
>100	0.08383368375078466	3.945253588611651
>500	0.0062605728334625115	1.1040433281894837
>1k	0.001685538839560122	0.684145284539978
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4247	0.12933210792150784	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.0905160311517716E-5	0.0
2	0.0	0.0	0.0	6.0905160311517716E-5	0.0
3	0.0	0.0	0.0	9.135774046727656E-5	0.0
4	0.0	0.0	0.0	3.3497838171334744E-4	0.0
5	0.0	0.0	0.0	3.3497838171334744E-4	0.0
6	0.0	0.0	0.0	6.39504183270936E-4	0.0
7	0.0	0.0	0.0	7.004093435824538E-4	0.0
8	0.0	0.0	0.0	7.004093435824538E-4	0.0
9	0.0	0.0	0.0	8.831248245170069E-4	0.0
10	0.0	0.0	0.0	8.831248245170069E-4	0.0
11	0.0	0.0	0.0	0.0012181032062303543	0.0
12	0.0	0.0	0.0	0.0016139867482552195	0.0
13	0.0	0.0	0.0	0.0016139867482552195	0.0
14	0.0	0.0	0.0	0.0016139867482552195	0.0
15	0.0	0.0	0.0	0.0016139867482552195	0.0
16	0.0	0.0	0.0	0.0016139867482552195	0.0
17	0.0	0.0	0.0	0.0018576073895012902	0.0
18	0.0	0.0	0.0	0.0018880599696570492	0.0
19	0.0	0.0	0.0	0.0018880599696570492	0.0
20	0.0	0.0	0.0	0.001948965129968567	0.0
21	0.0	0.0	0.0	0.001979417710124326	0.0
22	0.0	0.0	0.0	0.0031366157560431623	0.0
23	0.0	0.0	0.0	0.0035020467179122687	0.0
24	0.0	0.0	0.0	0.0037152147790025804	0.0
25	0.0	0.0	0.0	0.003837025099625616	0.0
26	0.0	0.0	0.0	0.004080645740871687	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3370	0.0	20.987919	1
GGTATCA	1160	0.0	20.968271	1
ACGCGTA	65	9.445517E-6	19.691595	27
GTCGCGA	90	4.3745058E-7	17.777136	30
CGTAGAC	70	3.6983137E-4	16.000395	3
CGCGTAG	60	0.0024437928	15.999421	28
TAGGACG	1685	0.0	15.668282	4
TCGCGAA	105	2.1886572E-6	15.237544	31
TTAGGAC	1265	0.0	14.92527	3
TATACGG	65	0.004160976	14.7707205	5
AGGACGT	1750	0.0	14.630048	5
CGTGATT	780	0.0	14.563576	28
ATACCCG	110	3.5433495E-6	14.546923	5
GTCCTAA	1290	0.0	14.513534	1
GTAGGAC	1735	0.0	14.478744	3
GGACGTG	1755	0.0	14.406012	6
TCGCGTA	170	7.585186E-10	14.11864	9
TAGGACC	980	0.0	13.878106	4
TATCAAC	5050	0.0	13.877359	2
CGCGTAA	190	2.7830538E-10	13.474428	10
>>END_MODULE