FastQCFastQC Report
Thu 2 Feb 2017
SRR4062738_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062738_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427900
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT33540.7838279971956065No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24310.5681233933161954No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA21710.5073615330684739No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC17930.41902313624678666No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT12920.30193970553867727No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10790.25216172002804393No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10030.23440056087870997No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA9470.22131339097920072No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG7170.1675625146062164No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG6020.14068707641972422No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA5670.13250759523253097No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG5640.13180649684505727No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA5530.12923580275765365No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG5400.1261977097452676No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG5260.12292591727039028No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC5240.12245851834540782No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC5170.12082262210796917No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5070.11848562748305677No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA4850.11334423930824959No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA4820.11264314092077589No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG4740.110773545220846No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG4710.11007244683337229No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA4700.10983874737088103No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC4570.10680065435849496No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4510.10539845758354757No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT4460.10422996027109137No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG4390.10259406403365272No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT4390.10259406403365272No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG4390.10259406403365272No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA4320.10095816779621407No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT4290.10025706940874037No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT4280.10002336994624914No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTTAT350.002054981422.8751892
GGCGAGC350.002066187822.8538068
GTATTAG1101.8189894E-1221.8456271
GTCCTAG453.5018855E-421.3601681
CGTTATT555.7870675E-520.3797132
TATAGCC400.004473660620.0040913
CGTGCGT400.004476688320.00175314
ATACCGT953.7791324E-818.523616
CCTTTAG802.7545884E-618.0226421
GTCTTGG450.00877764217.800141
AGAATTA904.3469117E-717.77933512
AATCGCT450.00883698917.77933515
TTAGCTC904.3522414E-717.7772584
CCGTGAT550.001364084117.45199627
CTAGTCC550.001364084117.4519968
CGAATGT652.0988655E-417.22825223
GTCCTAA753.191406E-517.0881351
TATTAGC854.931997E-616.954552
GTCTAAG957.564504E-716.8632891
CCCTCGA1051.1859083E-716.75945526
Produced by FastQC (version 0.11.5)