##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062738_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	427900
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.007856975928956	32.0	32.0	32.0	32.0	32.0
2	30.714926384669315	32.0	32.0	32.0	32.0	32.0
3	30.807936433746203	32.0	32.0	32.0	32.0	32.0
4	30.825683570927787	32.0	32.0	32.0	32.0	32.0
5	30.842133676092544	32.0	32.0	32.0	32.0	32.0
6	34.412757653657394	36.0	36.0	36.0	32.0	36.0
7	34.293542883851366	36.0	36.0	36.0	32.0	36.0
8	34.25942042533302	36.0	36.0	36.0	32.0	36.0
9	34.32967749474176	36.0	36.0	36.0	32.0	36.0
10	34.14155410142557	36.0	36.0	36.0	32.0	36.0
11	34.40022435148399	36.0	36.0	36.0	32.0	36.0
12	34.25249357326478	36.0	36.0	36.0	32.0	36.0
13	34.31631222248189	36.0	36.0	36.0	32.0	36.0
14	34.21814442626782	36.0	36.0	36.0	32.0	36.0
15	34.193152605749006	36.0	36.0	36.0	32.0	36.0
16	34.18680065435849	36.0	36.0	36.0	32.0	36.0
17	34.115340032717924	36.0	36.0	36.0	32.0	36.0
18	34.1559569992989	36.0	36.0	36.0	32.0	36.0
19	34.12466464127132	36.0	36.0	36.0	32.0	36.0
20	34.055440523486794	36.0	36.0	36.0	32.0	36.0
21	34.0132741294695	36.0	36.0	36.0	32.0	36.0
22	34.00749941575135	36.0	36.0	36.0	32.0	36.0
23	33.96015424164524	36.0	36.0	36.0	32.0	36.0
24	33.947931759756955	36.0	36.0	36.0	32.0	36.0
25	33.91833138583781	36.0	36.0	36.0	32.0	36.0
26	33.89090909090909	36.0	36.0	36.0	32.0	36.0
27	33.870369245150734	36.0	36.0	36.0	32.0	36.0
28	33.858604814208924	36.0	36.0	36.0	32.0	36.0
29	33.82371582145361	36.0	36.0	36.0	32.0	36.0
30	33.76050245384435	36.0	36.0	36.0	27.0	36.0
31	33.790558541715356	36.0	36.0	36.0	32.0	36.0
32	33.7207688712316	36.0	36.0	36.0	27.0	36.0
33	33.63842019163356	36.0	36.0	36.0	27.0	36.0
34	33.618322037859315	36.0	36.0	36.0	27.0	36.0
35	33.52802290254733	36.0	36.0	36.0	21.0	36.0
36	33.48319700864688	36.0	36.0	36.0	21.0	36.0
37	33.47422996027109	36.0	36.0	36.0	21.0	36.0
38	32.78986679130638	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	4.0
11	9.0
12	2.0
13	1.0
14	151.0
15	255.0
16	290.0
17	335.0
18	435.0
19	569.0
20	706.0
21	1026.0
22	1428.0
23	1947.0
24	2476.0
25	3524.0
26	4685.0
27	6653.0
28	9153.0
29	12338.0
30	16321.0
31	21447.0
32	28259.0
33	40914.0
34	87801.0
35	187169.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.21401613088149	17.47512161336893	12.254930635337722	24.05593162041186
2	15.664019753640673	20.981696082007538	38.2628581048851	25.091426059466688
3	18.505066087017493	26.679288059928247	28.41730065794856	26.398345195105705
4	12.149178875829946	16.564576327038086	35.66462330183763	35.62162149529434
5	13.97920074783828	37.55597102126665	32.720962841785465	15.743865389109605
6	32.26875438186492	35.70623977564852	17.895302640803926	14.129703201682636
7	28.762795045571394	31.802523954194907	21.413414349146997	18.021266651086705
8	28.696257761761533	31.725944673859953	20.146810345431984	19.430987218946527
9	28.246085534003274	14.604113110539846	18.801355456882447	38.34844589857443
10	16.446209143300507	26.89332504475366	30.776026062285872	25.884439749659965
11	36.411758107185925	22.0824413637086	22.04574986211474	19.460050666990735
12	25.216062222180675	24.606086667944265	28.73756093912865	21.440290170746415
13	30.058924860868608	20.203443867116373	25.369244541678647	24.36838673033637
14	24.53652197488851	20.373633873395473	25.687387227358993	29.402456924357022
15	25.186071735796833	26.66880166062011	24.6397782099712	23.50534839361185
16	24.846263699534642	26.05768938605984	25.2606680487936	23.83537886561192
17	23.335920187749533	25.589054800796628	26.54112631017943	24.533898701274413
18	24.6704621943011	24.289974571834566	28.369231919826486	22.670331314037842
19	24.613953531854822	25.991730998006375	26.91024930877407	22.484066161364737
20	25.30079370273075	23.362111679302963	27.738695111576465	23.59839950638982
21	27.186444639205376	24.207303534910896	25.067134092900968	23.539117732982763
22	25.069939957977596	24.505278509443354	26.131251066336038	24.293530466243016
23	23.32565939395356	24.476282852835656	26.582136471468136	25.615921281742647
24	23.543820939060193	26.36876156996204	26.29817311468053	23.78924437629724
25	24.722255335759584	24.550454512518087	26.20044364348641	24.526846508235923
26	23.328775288383998	25.639820950644555	27.317882729684563	23.713521031286888
27	24.815126443229094	25.36764362174543	26.42920581498621	23.388024120039265
28	23.471395784740366	24.907566246137883	27.18510589566087	24.43593207346088
29	22.84190401398608	25.67698326999397	27.69355901779579	23.78755369822416
30	23.621644234522055	25.57077518854453	27.651484167400902	23.156096409532513
31	23.515622626340058	25.8420138686012	25.90628527623851	24.736078228820237
32	23.007077015126303	26.394368303947047	25.91945104051755	24.679103640409103
33	22.982463931043025	25.44821799603142	26.517957934936813	25.051360137988738
34	23.44954445800085	26.016146147409557	27.043628255290503	23.490681139299088
35	24.786946094907744	25.616722686325748	26.79757658487329	22.798754633893218
36	23.33439293928458	26.833897417677967	26.034985695319655	23.796723947717798
37	24.317230450645432	26.899893187613504	26.093541473356503	22.689334888384565
38	23.223730184141704	26.82663475718489	26.004435854229136	23.94519920444427
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1154.0
1	779.0
2	404.0
3	404.0
4	772.0
5	1140.0
6	1140.0
7	1283.0
8	1426.0
9	1314.5
10	1203.0
11	1203.0
12	1528.5
13	1854.0
14	2125.5
15	2397.0
16	2397.0
17	3440.5
18	4484.0
19	4484.0
20	4998.5
21	5513.0
22	4916.0
23	4319.0
24	4319.0
25	4598.0
26	4877.0
27	4877.0
28	6321.0
29	7765.0
30	9524.5
31	11284.0
32	11284.0
33	13241.0
34	15198.0
35	15198.0
36	16347.5
37	17497.0
38	20138.5
39	22780.0
40	22780.0
41	24356.0
42	25932.0
43	29823.0
44	33714.0
45	33714.0
46	37314.0
47	40914.0
48	40914.0
49	40522.5
50	40131.0
51	38132.5
52	36134.0
53	36134.0
54	33930.0
55	31726.0
56	31726.0
57	30945.5
58	30165.0
59	26832.0
60	23499.0
61	23499.0
62	22727.5
63	21956.0
64	18178.5
65	14401.0
66	14401.0
67	12072.0
68	9743.0
69	9743.0
70	7893.0
71	6043.0
72	4745.5
73	3448.0
74	3448.0
75	2649.5
76	1851.0
77	1851.0
78	1807.0
79	1763.0
80	1411.5
81	1060.0
82	1060.0
83	1055.0
84	1050.0
85	1050.0
86	698.5
87	347.0
88	312.5
89	278.0
90	278.0
91	195.5
92	113.0
93	100.0
94	87.0
95	87.0
96	74.0
97	61.0
98	61.0
99	125.0
100	189.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1217574199579341
2	0.05421827529796682
3	0.012853470437017993
4	0.0025706940874035992
5	0.0
6	0.0
7	0.0
8	2.3369946249123628E-4
9	0.0
10	4.6739892498247256E-4
11	0.0018695956999298902
12	0.003271792474877308
13	0.01565786398691283
14	0.01121757419957934
15	0.025239541949053517
16	0.013320869362000467
17	0.02243514839915868
18	0.006543584949754616
19	0.008646880112175742
20	0.007478382799719561
21	0.005842486562280907
22	0.007712082262210797
23	0.010516475812105634
24	0.015891563449404067
25	0.018462257536807664
26	0.019864454311755084
27	0.00934797849964945
28	0.006076186024772143
29	0.010750175274596869
30	0.003972890862351017
31	0.006309885487263379
32	0.008413180649684505
33	0.009114279037158215
34	0.013554568824491705
35	0.017293760224351484
36	0.015891563449404067
37	0.010983874737088105
38	0.005375087637298434
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	427900.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.19639042626993
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.57883653944175	45.028573121518825
2	11.885803760662762	13.121069298071383
3	3.1244346378431054	5.1737254239524795
4	1.159319217914396	2.5596094472272366
5	0.5225028855755636	1.4420136635540723
6	0.2920145757669591	0.9670890320516795
7	0.20737532634295752	0.8012458634320716
8	0.14103680014460176	0.6227777828202593
9	0.0982799339731549	0.48822278459852597
>10	0.7394610988823306	8.294772658503131
>50	0.1318257677264663	5.117278994302073
>100	0.11147966852638416	11.777556112482099
>500	0.004662647733042684	1.5357452138964323
>1k	0.0029671394664817076	3.0703206035897135
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3354	0.7838279971956065	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2431	0.5681233933161954	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2171	0.5073615330684739	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1793	0.41902313624678666	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1292	0.30193970553867727	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1079	0.25216172002804393	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1003	0.23440056087870997	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	947	0.22131339097920072	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	717	0.1675625146062164	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	602	0.14068707641972422	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	567	0.13250759523253097	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	564	0.13180649684505727	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	553	0.12923580275765365	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	540	0.1261977097452676	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	526	0.12292591727039028	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	524	0.12245851834540782	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	517	0.12082262210796917	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	507	0.11848562748305677	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	485	0.11334423930824959	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	482	0.11264314092077589	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	474	0.110773545220846	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	471	0.11007244683337229	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	470	0.10983874737088103	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	457	0.10680065435849496	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	451	0.10539845758354757	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	446	0.10422996027109137	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	439	0.10259406403365272	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	439	0.10259406403365272	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	439	0.10259406403365272	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	432	0.10095816779621407	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	429	0.10025706940874037	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	428	0.10002336994624914	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	2.3369946249123628E-4
7	0.0	0.0	0.0	0.0	2.3369946249123628E-4
8	0.0	0.0	0.0	0.0	2.3369946249123628E-4
9	0.0	0.0	0.0	0.0	2.3369946249123628E-4
10	0.0	0.0	0.0	0.0	2.3369946249123628E-4
11	0.0	0.0	0.0	0.0	2.3369946249123628E-4
12	0.0	0.0	0.0	0.0	4.6739892498247256E-4
13	0.0	0.0	0.0	0.0	4.6739892498247256E-4
14	0.0	0.0	0.0	0.0	4.6739892498247256E-4
15	0.0	0.0	0.0	2.3369946249123628E-4	4.6739892498247256E-4
16	0.0	0.0	0.0	2.3369946249123628E-4	4.6739892498247256E-4
17	0.0	0.0	0.0	4.6739892498247256E-4	4.6739892498247256E-4
18	0.0	0.0	0.0	4.6739892498247256E-4	4.6739892498247256E-4
19	0.0	0.0	0.0	4.6739892498247256E-4	4.6739892498247256E-4
20	0.0	0.0	0.0	4.6739892498247256E-4	4.6739892498247256E-4
21	0.0	0.0	0.0	0.0014021967749474175	4.6739892498247256E-4
22	0.0	0.0	0.0	0.0023369946249123625	4.6739892498247256E-4
23	0.0	0.0	0.0	0.007712082262210797	7.010983874737088E-4
24	0.0	0.0	0.0	0.01191867258705305	7.010983874737088E-4
25	0.0	0.0	0.0	0.013087169899509232	7.010983874737088E-4
26	0.0	0.0	0.0	0.015891563449404067	7.010983874737088E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACTTAT	35	0.0020549814	22.875189	2
GGCGAGC	35	0.0020661878	22.853806	8
GTATTAG	110	1.8189894E-12	21.845627	1
GTCCTAG	45	3.5018855E-4	21.360168	1
CGTTATT	55	5.7870675E-5	20.379713	2
TATAGCC	40	0.0044736606	20.004091	3
CGTGCGT	40	0.0044766883	20.001753	14
ATACCGT	95	3.7791324E-8	18.52361	6
CCTTTAG	80	2.7545884E-6	18.022642	1
GTCTTGG	45	0.008777642	17.80014	1
AGAATTA	90	4.3469117E-7	17.779335	12
AATCGCT	45	0.008836989	17.779335	15
TTAGCTC	90	4.3522414E-7	17.777258	4
CCGTGAT	55	0.0013640841	17.451996	27
CTAGTCC	55	0.0013640841	17.451996	8
CGAATGT	65	2.0988655E-4	17.228252	23
GTCCTAA	75	3.191406E-5	17.088135	1
TATTAGC	85	4.931997E-6	16.95455	2
GTCTAAG	95	7.564504E-7	16.863289	1
CCCTCGA	105	1.1859083E-7	16.759455	26
>>END_MODULE