FastQCFastQC Report
Thu 2 Feb 2017
SRR4062738_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062738_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427900
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT38250.8939004440289787No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT29620.6922178078990419No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA22640.5290955830801589No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC21720.5075952325309652No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT17260.40336527225987384No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12460.2911895302640804No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA8490.19841084365505962No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG7890.18438887590558542No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG6110.14279037158214536No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG6100.14255667211965412No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA5900.13788268286982938No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA5880.13741528394484692No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5710.13344239308249592No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5660.13227389577003973No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5550.12970320168263613No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC5460.127599906520215No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5370.12549661135779389No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA5310.12409441458284647No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC5120.11965412479551298No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG5090.11895302640803926No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG5090.11895302640803926No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT5070.11848562748305677No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC4890.11427903715821454No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG4880.11404533769572331No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA4760.11124094414582845No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC4700.10983874737088103No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG4620.10796915167095116No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4570.10680065435849496No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG4550.1063332554335125No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA4470.1044636597335826No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT4450.10399626080860014No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT4440.10376256134610891No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC4380.10236036457116149No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT4380.10236036457116149No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG4320.10095816779621407No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT4300.1004907688712316No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC4290.10025706940874037No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA4280.10002336994624914No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAC350.002064434622.8571431
TCTAGCG1250.020.4828
TATGGCG951.715307E-920.21052616
ACTGCAC400.004478961720.08
GGTCGTA400.004478961720.029
GTAGGAC659.411142E-619.6923083
AACGAAT507.1858417E-419.20000331
TAGCGGC1401.8189894E-1218.28571530
ATATGGC1750.018.28571515
ATAACGA802.7878687E-618.012
GGCGAGA450.00884145417.77777919
TAAGCTG450.00884145417.7777795
CTAGCGG1453.6379788E-1217.65517229
TGGCGAG1006.784467E-817.60000218
GAAAATC753.2260334E-517.06666831
AGCGGCG1601.8189894E-1217.031
AATATGG1700.016.94117714
ATCGTTT957.6646756E-716.84210629
ATGGCGA1151.8313585E-816.69565217
GATATAC1151.8313585E-816.6956521
Produced by FastQC (version 0.11.5)