##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062738_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	427900
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.99953026408039	32.0	32.0	32.0	32.0	32.0
2	31.25281607852302	32.0	32.0	32.0	32.0	32.0
3	31.35740827296097	32.0	32.0	32.0	32.0	32.0
4	31.47191633559243	32.0	32.0	32.0	32.0	32.0
5	31.372748305678897	32.0	32.0	32.0	32.0	32.0
6	34.8673825660201	36.0	36.0	36.0	32.0	36.0
7	34.906466464127135	36.0	36.0	36.0	36.0	36.0
8	34.84752979668147	36.0	36.0	36.0	32.0	36.0
9	34.956728207525124	36.0	36.0	36.0	32.0	36.0
10	34.79711381163823	36.0	36.0	36.0	32.0	36.0
11	34.971035288618836	36.0	36.0	36.0	36.0	36.0
12	34.87056087870998	36.0	36.0	36.0	32.0	36.0
13	34.911341434914696	36.0	36.0	36.0	32.0	36.0
14	34.843680766534234	36.0	36.0	36.0	32.0	36.0
15	34.82693620004674	36.0	36.0	36.0	32.0	36.0
16	34.83818649217107	36.0	36.0	36.0	32.0	36.0
17	34.80692451507362	36.0	36.0	36.0	32.0	36.0
18	34.77515541014256	36.0	36.0	36.0	32.0	36.0
19	34.76596401028278	36.0	36.0	36.0	32.0	36.0
20	34.72742463192335	36.0	36.0	36.0	32.0	36.0
21	34.71285814442627	36.0	36.0	36.0	32.0	36.0
22	34.686735218509	36.0	36.0	36.0	32.0	36.0
23	34.60881748071979	36.0	36.0	36.0	32.0	36.0
24	34.58715354054686	36.0	36.0	36.0	32.0	36.0
25	34.56683337228324	36.0	36.0	36.0	32.0	36.0
26	34.49510399626081	36.0	36.0	36.0	32.0	36.0
27	34.46945548025239	36.0	36.0	36.0	32.0	36.0
28	34.430070109838745	36.0	36.0	36.0	32.0	36.0
29	34.36932928254265	36.0	36.0	36.0	32.0	36.0
30	34.32714652956298	36.0	36.0	36.0	32.0	36.0
31	34.30700630988549	36.0	36.0	36.0	32.0	36.0
32	34.24950923112877	36.0	36.0	36.0	32.0	36.0
33	34.17403832671185	36.0	36.0	36.0	32.0	36.0
34	34.14544753447067	36.0	36.0	36.0	32.0	36.0
35	34.083799953260105	36.0	36.0	36.0	32.0	36.0
36	34.03359196073849	36.0	36.0	36.0	32.0	36.0
37	33.987527459686845	36.0	36.0	36.0	32.0	36.0
38	33.53756718859547	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	5.0
21	11.0
22	46.0
23	144.0
24	360.0
25	864.0
26	1850.0
27	3506.0
28	6054.0
29	9356.0
30	14068.0
31	19675.0
32	27600.0
33	42092.0
34	96134.0
35	206131.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.535766204991326	18.593611958996124	13.124558853088145	24.746062982924403
2	14.835709277868661	22.00560878709979	37.39121290021033	25.76746903482122
3	17.680766534236973	27.13811638233232	28.876840383267115	26.304276700163587
4	11.691516709511568	17.464594531432578	35.49193736854405	35.35195139051181
5	13.421126431409208	37.932928254265015	32.63122224818883	16.01472306613695
6	32.32702109609978	36.27538274218916	17.466037546243392	13.931558615467669
7	28.328413948151315	31.73202087408477	21.761677405182063	18.177887772581848
8	28.523619332960966	32.08620887095078	19.96279426302493	19.42737753306333
9	28.12965226475462	14.190795147271567	18.585921852469017	39.093630735504796
10	16.30485841218798	27.164948574072735	30.85228454511249	25.677908468626796
11	36.54860534956823	21.812169157600756	22.094721712609697	19.54450378022132
12	24.895594521136996	24.706998614158948	28.78880296518571	21.608603899518343
13	30.14536106566955	20.14980135545688	25.458518345407803	24.246319233465762
14	24.4293059125964	20.12830100490769	25.898574433278803	29.54381864921711
15	25.381163823323206	26.578639869128303	24.6106566954896	23.429539612058893
16	24.74503388642206	25.889927553166626	25.309184388875906	24.055854171535408
17	23.122458518345407	25.608553400327178	26.68871231596167	24.580275765365737
18	24.66487497078757	23.847160551530735	28.638700630988552	22.84926384669315
19	24.663239074550127	25.522551998130403	27.312923580275765	22.5012853470437
20	25.289787333489134	23.070810937134844	28.07758822154709	23.561813507828933
21	26.994391212900208	24.11311053984576	25.384903014723065	23.507595232530964
22	25.099380928677096	24.254789097427196	26.39361157656363	24.252218397332083
23	23.136768256060424	23.966403286756922	27.079053701924988	25.817774755257666
24	23.42907221313391	26.0079457817247	26.75251226922178	23.810469735919607
25	24.64851600841318	24.017527459686843	26.667445664874972	24.666510867025007
26	23.375087637298435	25.109838747370883	27.6639401729376	23.85113344239308
27	24.887590558541714	24.92077588221547	26.739892498247254	23.45174106099556
28	23.464360831970087	24.401729376022434	27.730778219210094	24.40313157279738
29	23.123860715120355	24.97008646880112	28.155877541481654	23.75017527459687
30	23.778452909558307	24.90745501285347	28.05725636831035	23.25683570927787
31	23.49684505725637	25.154007945781725	26.58074316429072	24.768403832671186
32	23.149801355456884	25.565085300303807	26.645711614863288	24.639401729376022
33	23.200747838279973	24.718625847160553	27.104463659733586	24.976162654825895
34	23.54615564384202	25.242346342603412	27.71395185791073	23.497546155643843
35	25.144426267819586	24.861649918205188	27.147931759756954	22.845992054218275
36	23.636597335826128	26.025706940874038	26.551063332554335	23.786632390745503
37	24.7520448702968	26.070577237672353	26.4559476513204	22.721430240710447
38	23.565552699228792	25.659032484225285	26.562514606216403	24.212900210329515
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	793.0
1	504.5
2	216.0
3	216.0
4	667.0
5	1118.0
6	1118.0
7	1318.5
8	1519.0
9	1451.5
10	1384.0
11	1384.0
12	1675.5
13	1967.0
14	2226.5
15	2486.0
16	2486.0
17	3779.0
18	5072.0
19	5072.0
20	5382.0
21	5692.0
22	5091.5
23	4491.0
24	4491.0
25	4474.0
26	4457.0
27	4457.0
28	5987.0
29	7517.0
30	9219.5
31	10922.0
32	10922.0
33	13184.5
34	15447.0
35	15447.0
36	16191.0
37	16935.0
38	19577.0
39	22219.0
40	22219.0
41	23625.5
42	25032.0
43	29145.0
44	33258.0
45	33258.0
46	37727.5
47	42197.0
48	42197.0
49	41895.0
50	41593.0
51	39008.5
52	36424.0
53	36424.0
54	34081.5
55	31739.0
56	31739.0
57	30881.5
58	30024.0
59	26735.0
60	23446.0
61	23446.0
62	22859.5
63	22273.0
64	18360.5
65	14448.0
66	14448.0
67	12156.5
68	9865.0
69	9865.0
70	8023.5
71	6182.0
72	4800.5
73	3419.0
74	3419.0
75	2566.5
76	1714.0
77	1714.0
78	1746.0
79	1778.0
80	1371.5
81	965.0
82	965.0
83	917.0
84	869.0
85	869.0
86	542.0
87	215.0
88	189.0
89	163.0
90	163.0
91	97.5
92	32.0
93	22.0
94	12.0
95	12.0
96	11.0
97	10.0
98	10.0
99	8.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008880579574666978
2	0.0
3	0.0
4	0.0
5	0.0
6	2.3369946249123628E-4
7	2.3369946249123628E-4
8	0.0021032951624211263
9	0.003972890862351017
10	7.010983874737088E-4
11	0.003505491937368544
12	2.3369946249123628E-4
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	2.3369946249123628E-4
23	2.3369946249123628E-4
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	427900.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.826758626567326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.11603805263246	42.322706043232984
2	12.782236899044358	13.504882867468373
3	3.420631694334855	5.421026546010403
4	1.2767634281843938	2.697890937757024
5	0.5826733151486577	1.539037128874997
6	0.3175541437584434	1.006521366191613
7	0.2125082186895835	0.7858284262413489
8	0.14700420438128312	0.6212604497552492
9	0.10438269971269852	0.4962779714260966
>10	0.7649956658648751	8.259423462332519
>50	0.14559585558968074	5.4146685141433375
>100	0.11985024697897065	12.402313356391542
>500	0.007102236858013076	2.199540542313342
>1k	0.002663338821754903	3.3286223878611825
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3825	0.8939004440289787	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2962	0.6922178078990419	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2264	0.5290955830801589	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2172	0.5075952325309652	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1726	0.40336527225987384	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1246	0.2911895302640804	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	849	0.19841084365505962	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	789	0.18438887590558542	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	611	0.14279037158214536	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	610	0.14255667211965412	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	590	0.13788268286982938	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	588	0.13741528394484692	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	571	0.13344239308249592	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	566	0.13227389577003973	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	555	0.12970320168263613	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	546	0.127599906520215	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	537	0.12549661135779389	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	531	0.12409441458284647	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	512	0.11965412479551298	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	509	0.11895302640803926	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	509	0.11895302640803926	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	507	0.11848562748305677	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	489	0.11427903715821454	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	488	0.11404533769572331	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	476	0.11124094414582845	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	470	0.10983874737088103	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	462	0.10796915167095116	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	457	0.10680065435849496	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	455	0.1063332554335125	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	447	0.1044636597335826	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	445	0.10399626080860014	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	444	0.10376256134610891	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	438	0.10236036457116149	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	438	0.10236036457116149	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	432	0.10095816779621407	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	430	0.1004907688712316	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	429	0.10025706940874037	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	428	0.10002336994624914	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	2.3369946249123628E-4
7	0.0	0.0	0.0	0.0	2.3369946249123628E-4
8	0.0	0.0	0.0	0.0	2.3369946249123628E-4
9	0.0	0.0	0.0	0.0	2.3369946249123628E-4
10	0.0	0.0	0.0	0.0	2.3369946249123628E-4
11	0.0	0.0	0.0	0.0	2.3369946249123628E-4
12	0.0	0.0	0.0	0.0	4.6739892498247256E-4
13	0.0	0.0	0.0	0.0	4.6739892498247256E-4
14	0.0	0.0	0.0	0.0	4.6739892498247256E-4
15	0.0	0.0	0.0	2.3369946249123628E-4	4.6739892498247256E-4
16	0.0	0.0	0.0	2.3369946249123628E-4	4.6739892498247256E-4
17	0.0	0.0	0.0	4.6739892498247256E-4	4.6739892498247256E-4
18	0.0	0.0	0.0	4.6739892498247256E-4	4.6739892498247256E-4
19	0.0	0.0	0.0	4.6739892498247256E-4	4.6739892498247256E-4
20	0.0	0.0	0.0	4.6739892498247256E-4	7.010983874737088E-4
21	0.0	0.0	0.0	0.0014021967749474175	9.347978499649451E-4
22	0.0	0.0	0.0	0.0023369946249123625	9.347978499649451E-4
23	0.0	0.0	0.0	0.007712082262210797	9.347978499649451E-4
24	0.0	0.0	0.0	0.012152372049544287	9.347978499649451E-4
25	0.0	0.0	0.0	0.013554568824491704	9.347978499649451E-4
26	0.0	0.0	0.0	0.016125262911895304	9.347978499649451E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTTAC	35	0.0020644346	22.857143	1
TCTAGCG	125	0.0	20.48	28
TATGGCG	95	1.715307E-9	20.210526	16
ACTGCAC	40	0.0044789617	20.0	8
GGTCGTA	40	0.0044789617	20.0	29
GTAGGAC	65	9.411142E-6	19.692308	3
AACGAAT	50	7.1858417E-4	19.200003	31
TAGCGGC	140	1.8189894E-12	18.285715	30
ATATGGC	175	0.0	18.285715	15
ATAACGA	80	2.7878687E-6	18.0	12
GGCGAGA	45	0.008841454	17.777779	19
TAAGCTG	45	0.008841454	17.777779	5
CTAGCGG	145	3.6379788E-12	17.655172	29
TGGCGAG	100	6.784467E-8	17.600002	18
GAAAATC	75	3.2260334E-5	17.066668	31
AGCGGCG	160	1.8189894E-12	17.0	31
AATATGG	170	0.0	16.941177	14
ATCGTTT	95	7.6646756E-7	16.842106	29
ATGGCGA	115	1.8313585E-8	16.695652	17
GATATAC	115	1.8313585E-8	16.695652	1
>>END_MODULE