FastQCFastQC Report
Thu 2 Feb 2017
SRR4062737_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062737_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3314753
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT56770.1712646462647443No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG38790.11702229396881156No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA38500.11614741731887715No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA38350.11569489491373866No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG38220.11530270882928531No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG37520.11319093760530574No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC36440.10993277628830865No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT34940.10540755223692384No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG34070.10278292228712063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT8100.018.5645756
ACCGTCG8050.018.2840928
TACCGTC8350.017.4355817
CGTCGTA8850.016.99489410
CCGTCGT8800.016.727819
TACCGTA1001.317414E-615.9985837
TCTAGCG6550.015.63314528
GTCGTAG9250.015.56806611
TAACGCA1052.1922715E-615.2351374
AGATACC10250.014.9824554
TTAGGAC11400.014.7353133
TAGGACG18300.014.6856724
CTAGCGG6950.014.50318629
ACCGTAT1002.3302522E-514.3987258
AACCGCG6000.014.3987247
GTATCAA58600.014.267441
CGCTTCG9100.014.24135532
CAAGACG9100.014.2389934
GTAGGAC20450.014.2381323
CGCGGTC5550.014.12657810
Produced by FastQC (version 0.11.5)