##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062737_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3314753
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27119275553865	32.0	32.0	32.0	32.0	32.0
2	30.850421434115905	32.0	32.0	32.0	32.0	32.0
3	30.89788892264371	32.0	32.0	32.0	32.0	32.0
4	30.976567786498723	32.0	32.0	32.0	32.0	32.0
5	30.86694031199308	32.0	32.0	32.0	32.0	32.0
6	34.5398323796675	36.0	36.0	36.0	32.0	36.0
7	34.474202451887066	36.0	36.0	36.0	32.0	36.0
8	34.453825367983676	36.0	36.0	36.0	32.0	36.0
9	34.60297132244846	36.0	36.0	36.0	32.0	36.0
10	34.301462884263174	36.0	36.0	36.0	32.0	36.0
11	34.59445424742054	36.0	36.0	36.0	32.0	36.0
12	34.39959749640471	36.0	36.0	36.0	32.0	36.0
13	34.48758625454144	36.0	36.0	36.0	32.0	36.0
14	34.38402891557833	36.0	36.0	36.0	32.0	36.0
15	34.325942837973145	36.0	36.0	36.0	32.0	36.0
16	34.35066112014983	36.0	36.0	36.0	32.0	36.0
17	34.27742972100787	36.0	36.0	36.0	32.0	36.0
18	34.30695710962476	36.0	36.0	36.0	32.0	36.0
19	34.28868515995008	36.0	36.0	36.0	32.0	36.0
20	34.27179280024786	36.0	36.0	36.0	32.0	36.0
21	34.255743942308825	36.0	36.0	36.0	32.0	36.0
22	34.22793794892108	36.0	36.0	36.0	32.0	36.0
23	34.164537448189954	36.0	36.0	36.0	32.0	36.0
24	34.15211027790004	36.0	36.0	36.0	32.0	36.0
25	34.12977920225127	36.0	36.0	36.0	32.0	36.0
26	34.08581423713924	36.0	36.0	36.0	32.0	36.0
27	34.087803525632225	36.0	36.0	36.0	32.0	36.0
28	34.05819830316165	36.0	36.0	36.0	32.0	36.0
29	34.02414961235422	36.0	36.0	36.0	32.0	36.0
30	33.99551912314432	36.0	36.0	36.0	32.0	36.0
31	34.01214177949307	36.0	36.0	36.0	32.0	36.0
32	33.96199716841647	36.0	36.0	36.0	32.0	36.0
33	33.937206482654965	36.0	36.0	36.0	32.0	36.0
34	33.94613444802675	36.0	36.0	36.0	32.0	36.0
35	33.905575769898995	36.0	36.0	36.0	32.0	36.0
36	33.85599198492316	36.0	36.0	36.0	32.0	36.0
37	33.85195789852215	36.0	36.0	36.0	32.0	36.0
38	33.25690089125796	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	2.0
7	8.0
8	3.0
9	11.0
10	28.0
11	33.0
12	13.0
13	13.0
14	220.0
15	613.0
16	818.0
17	1024.0
18	1252.0
19	1804.0
20	2428.0
21	3707.0
22	5856.0
23	9047.0
24	14374.0
25	21485.0
26	32044.0
27	46548.0
28	65026.0
29	88359.0
30	119742.0
31	160550.0
32	218765.0
33	325344.0
34	717810.0
35	1477825.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.191724802847446	17.436202734054195	11.584828986447526	25.787243476650833
2	16.130105281840745	19.828749105395637	38.4887396816763	25.552405931087318
3	18.436860263912585	24.596701318959497	28.816707926579983	28.149730490547935
4	12.306020945725386	16.035028609251864	36.90008211998689	34.758868325035856
5	14.307336276343264	36.82676234415477	34.0689651427394	14.796936236762564
6	33.21231831154178	36.27605785069116	17.509608596752688	13.002015241014378
7	29.696309105477575	30.720246944567798	21.64973427876354	17.933709671191085
8	27.432852219826525	33.74164074960909	19.906200816293037	18.91930621427134
9	27.654843829521624	14.514428418734122	18.8294839147409	39.00124383700335
10	15.647750708051209	26.685656638304394	32.12473889291804	25.541853760726358
11	36.86261365696752	21.10949172003769	23.355795973619244	18.672098649375553
12	25.19404705158297	23.523951174479436	29.29869126848606	21.983310505451538
13	29.445160028929124	19.72907156760071	25.43285821138476	25.392910192085406
14	23.40503397974038	20.41011019844772	25.39729519758031	30.787560624231592
15	24.7650955474747	28.115088471448612	22.398564637914163	24.721251343162525
16	25.350643629852115	25.700268977388095	24.633471166016225	24.315616226743565
17	23.645271591408335	26.163846792609508	25.859389709893737	24.331491906088416
18	24.581153064524695	24.86046480756513	27.135748282736614	23.42263384517356
19	25.561983181837928	25.012822400401628	25.584067880882618	23.841126536877827
20	25.609351010136816	24.693540693747057	25.292867721751755	24.404240574364373
21	26.636460564020226	24.453034820286966	24.410344413530353	24.50016020216246
22	25.46199695642572	25.00238344932787	24.996741613577093	24.538877980669323
23	23.9327167837242	24.64423389944748	25.804510951534592	25.618538365293723
24	24.785234202735534	25.338864100741393	25.444708216617162	24.43119347990591
25	24.769529669443706	24.84945843658849	25.612115861409734	24.768896032558075
26	24.176308609767887	25.789625504576414	26.106417119721826	23.927648765933874
27	24.950120335727174	25.36985473122909	25.032667295624616	24.647357637419116
28	23.979657102033084	25.28404873992781	25.726428630906195	25.009865527132913
29	24.28373159546223	25.166877866280473	25.622254405020517	24.927136133236786
30	24.096240512584423	25.382528613846272	26.04458866596634	24.476642207602968
31	25.059721098397564	24.931318474108586	24.931469323056294	25.077491104437556
32	24.246275046477063	25.457273381689998	24.92570608633843	25.37074548549451
33	23.90323935401184	25.040247543512002	25.77722411425234	25.279288988223826
34	24.7825904614667	25.11441852999381	26.00433204892518	24.098658959614312
35	25.464825007588427	25.0752053702518	25.666378417129863	23.793591205029905
36	24.154344930173053	25.63781109838833	25.349964471760707	24.857879499677907
37	25.158766866669623	25.487780630940087	24.98878396452384	24.36466853786645
38	24.422934022492818	25.5432606773309	25.428464454956284	24.60534084522
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	329.0
1	338.0
2	347.0
3	347.0
4	872.0
5	1397.0
6	1397.0
7	1642.5
8	1888.0
9	1841.0
10	1794.0
11	1794.0
12	2423.5
13	3053.0
14	4263.0
15	5473.0
16	5473.0
17	8137.5
18	10802.0
19	10802.0
20	14262.0
21	17722.0
22	21289.0
23	24856.0
24	24856.0
25	31769.0
26	38682.0
27	38682.0
28	50170.0
29	61658.0
30	77556.5
31	93455.0
32	93455.0
33	118641.5
34	143828.0
35	143828.0
36	156948.0
37	170068.0
38	188530.0
39	206992.0
40	206992.0
41	213766.0
42	220540.0
43	241327.0
44	262114.0
45	262114.0
46	265585.0
47	269056.0
48	269056.0
49	284512.5
50	299969.0
51	299007.0
52	298045.0
53	298045.0
54	282293.5
55	266542.0
56	266542.0
57	256334.0
58	246126.0
59	220340.0
60	194554.0
61	194554.0
62	184392.5
63	174231.0
64	142710.0
65	111189.0
66	111189.0
67	92064.0
68	72939.0
69	72939.0
70	58982.0
71	45025.0
72	35234.5
73	25444.0
74	25444.0
75	18948.0
76	12452.0
77	12452.0
78	12358.5
79	12265.0
80	9666.0
81	7067.0
82	7067.0
83	7246.0
84	7425.0
85	7425.0
86	4878.0
87	2331.0
88	2037.5
89	1744.0
90	1744.0
91	1282.5
92	821.0
93	707.0
94	593.0
95	593.0
96	529.0
97	465.0
98	465.0
99	968.5
100	1472.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12043129608752146
2	0.055388742388950245
3	0.012006927816341066
4	0.00274530259117346
5	1.8100896205539295E-4
6	3.921860844533514E-4
7	3.0168160342565495E-5
8	3.620179241107859E-4
9	3.016816034256549E-4
10	6.335313671938755E-4
11	0.0019307622619241917
12	0.002805638911858591
13	0.013454999512784211
14	0.008386748575233208
15	0.02250544761555386
16	0.010498519799212793
17	0.01982048134506553
18	0.005339764380634092
19	0.007270526642558284
20	0.00609396838919823
21	0.005762118625430009
22	0.0069085087184474975
23	0.00862809385797373
24	0.013424831352441645
25	0.016652824509096154
26	0.017708710121085946
27	0.008326412254548077
28	0.005460437022004354
29	0.010649360600925618
30	0.002986647873913984
31	0.005400100701319223
32	0.006486154473651581
33	0.007994562490779856
34	0.011644909892230281
35	0.01493323936956992
36	0.014722062247171961
37	0.00911078442345478
38	0.005550941503032051
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	3314753.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.92457598978654
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.52272281143576	38.20364489944897
2	13.709260586327607	13.688580438117965
3	4.499416009300707	6.738943093979858
4	1.83406538340754	3.662597464166669
5	0.9915526683131028	2.4751423268536556
6	0.5649996361574123	1.692442036172549
7	0.34990136630930424	1.2228074145867298
8	0.24947913263131566	0.9964111931934524
9	0.2002385642144593	0.8997142873689471
>10	0.9131634158146638	8.189279398996813
>50	0.07370317636592835	2.5618808034836213
>100	0.06952657205851359	7.476513116217051
>500	0.013632775060614392	4.685122244436303
>1k	0.008277042000217222	7.334429055735798
>5k	6.0860602942773695E-5	0.17249222724166957
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5677	0.1712646462647443	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	3879	0.11702229396881156	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	3850	0.11614741731887715	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3835	0.11569489491373866	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3822	0.11530270882928531	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3752	0.11319093760530574	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3644	0.10993277628830865	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3494	0.10540755223692384	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	3407	0.10278292228712063	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	6.033632068513099E-5	0.0	6.033632068513099E-5	0.0
11	0.0	9.050448102769649E-5	0.0	9.050448102769649E-5	0.0
12	0.0	9.050448102769649E-5	0.0	9.050448102769649E-5	1.2067264137026198E-4
13	0.0	9.050448102769649E-5	0.0	9.050448102769649E-5	1.8100896205539298E-4
14	0.0	9.050448102769649E-5	0.0	9.050448102769649E-5	1.8100896205539298E-4
15	0.0	9.050448102769649E-5	0.0	9.050448102769649E-5	4.525224051384824E-4
16	0.0	9.050448102769649E-5	0.0	1.2067264137026198E-4	4.826905654810479E-4
17	0.0	9.050448102769649E-5	0.0	1.2067264137026198E-4	4.826905654810479E-4
18	0.0	9.050448102769649E-5	0.0	1.5084080171282746E-4	5.430268861661789E-4
19	0.0	9.050448102769649E-5	0.0	1.8100896205539298E-4	5.731950465087444E-4
20	0.0	9.050448102769649E-5	0.0	2.1117712239795847E-4	6.938676878790064E-4
21	0.0	9.050448102769649E-5	0.0	3.3184976376822044E-4	7.542040085641373E-4
22	0.0	9.050448102769649E-5	0.0	4.826905654810479E-4	8.145403292492684E-4
23	0.0	9.050448102769649E-5	0.0	7.542040085641373E-4	8.145403292492684E-4
24	0.0	1.2067264137026198E-4	0.0	0.0014179035361005783	8.145403292492684E-4
25	0.0	1.2067264137026198E-4	0.0	0.0018402577808964951	8.145403292492684E-4
26	0.0	1.2067264137026198E-4	0.0	0.002443620987747805	8.145403292492684E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCGT	810	0.0	18.564575	6
ACCGTCG	805	0.0	18.284092	8
TACCGTC	835	0.0	17.435581	7
CGTCGTA	885	0.0	16.994894	10
CCGTCGT	880	0.0	16.72781	9
TACCGTA	100	1.317414E-6	15.998583	7
TCTAGCG	655	0.0	15.633145	28
GTCGTAG	925	0.0	15.568066	11
TAACGCA	105	2.1922715E-6	15.235137	4
AGATACC	1025	0.0	14.982455	4
TTAGGAC	1140	0.0	14.735313	3
TAGGACG	1830	0.0	14.685672	4
CTAGCGG	695	0.0	14.503186	29
ACCGTAT	100	2.3302522E-5	14.398725	8
AACCGCG	600	0.0	14.398724	7
GTATCAA	5860	0.0	14.26744	1
CGCTTCG	910	0.0	14.241355	32
CAAGACG	910	0.0	14.238993	4
GTAGGAC	2045	0.0	14.238132	3
CGCGGTC	555	0.0	14.126578	10
>>END_MODULE