FastQCFastQC Report
Thu 2 Feb 2017
SRR4062737_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062737_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3314753
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT58790.17735861465394256No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT41720.12586156494918324No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG41570.12540904254404475No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA39750.11991843736169783No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA39550.11931507415484652No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC38320.11560439043271098No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA37600.11343228288804627No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC36970.11153168878646463No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG36300.10951042204351273No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC34100.10287342676814834No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG33280.10039963762005796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTT8250.016.29038829
CATCGTT8350.016.09529328
CGTTTAT8750.015.90806131
CGGCATC8800.015.63586225
TCTAGCG8550.015.15740928
CTAGCGG8700.015.07997729
ACCGTCG8400.014.85845858
GTATCAA54700.014.6551151
TCGTTTA9200.014.60822730
GTTTTCG10550.014.55877428
TACCGTC8250.014.5467447
TAGCGGC9000.014.39953930
GTATAGA4600.014.2614881
TTAGGAC12250.014.2373513
TTTTCGG10700.014.20515229
ATTTAGA9600.014.1672811
GTCCTAA11350.014.09752751
GGCATCG9800.013.87710626
ATACCGT8800.013.81940656
TGCACCG2201.8189894E-1213.8191985
Produced by FastQC (version 0.11.5)