##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062737_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3314753
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.180961598043655	32.0	32.0	32.0	32.0	32.0
2	31.235462793155328	32.0	32.0	32.0	32.0	32.0
3	31.342564287595486	32.0	32.0	32.0	32.0	32.0
4	31.43743198965353	32.0	32.0	32.0	32.0	32.0
5	31.37727939306488	32.0	32.0	32.0	32.0	32.0
6	34.879785612985344	36.0	36.0	36.0	36.0	36.0
7	34.90557516653579	36.0	36.0	36.0	36.0	36.0
8	34.83076748101593	36.0	36.0	36.0	32.0	36.0
9	34.97613698516903	36.0	36.0	36.0	36.0	36.0
10	34.7992815754296	36.0	36.0	36.0	32.0	36.0
11	34.98306359478369	36.0	36.0	36.0	36.0	36.0
12	34.87508390519595	36.0	36.0	36.0	32.0	36.0
13	34.93613430623639	36.0	36.0	36.0	36.0	36.0
14	34.87238868175095	36.0	36.0	36.0	32.0	36.0
15	34.84048193032784	36.0	36.0	36.0	32.0	36.0
16	34.86390599842583	36.0	36.0	36.0	32.0	36.0
17	34.8319274467811	36.0	36.0	36.0	32.0	36.0
18	34.82725922564969	36.0	36.0	36.0	32.0	36.0
19	34.82287518858871	36.0	36.0	36.0	32.0	36.0
20	34.81579803985395	36.0	36.0	36.0	32.0	36.0
21	34.80477761088081	36.0	36.0	36.0	32.0	36.0
22	34.78606007747787	36.0	36.0	36.0	32.0	36.0
23	34.71799165729694	36.0	36.0	36.0	32.0	36.0
24	34.705773854039805	36.0	36.0	36.0	32.0	36.0
25	34.68347777345703	36.0	36.0	36.0	32.0	36.0
26	34.615550238584895	36.0	36.0	36.0	32.0	36.0
27	34.59869951094395	36.0	36.0	36.0	32.0	36.0
28	34.570612651983424	36.0	36.0	36.0	32.0	36.0
29	34.52424207776567	36.0	36.0	36.0	32.0	36.0
30	34.50398114127961	36.0	36.0	36.0	32.0	36.0
31	34.499025417580135	36.0	36.0	36.0	32.0	36.0
32	34.46349184992064	36.0	36.0	36.0	32.0	36.0
33	34.42813054245671	36.0	36.0	36.0	32.0	36.0
34	34.41516245705185	36.0	36.0	36.0	32.0	36.0
35	34.38602122088735	36.0	36.0	36.0	32.0	36.0
36	34.360939714060144	36.0	36.0	36.0	32.0	36.0
37	34.34862838950595	36.0	36.0	36.0	32.0	36.0
38	33.921140730546135	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	4.0
20	12.0
21	63.0
22	251.0
23	822.0
24	2279.0
25	5633.0
26	11761.0
27	22926.0
28	39882.0
29	63400.0
30	95468.0
31	137994.0
32	197991.0
33	317252.0
34	765150.0
35	1653863.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.95164557993172	18.927012793842785	12.399241511124655	26.72210011510084
2	15.19452580629688	21.06980520117185	37.7528883750916	25.982780617439673
3	17.394267385835384	25.460780939032258	29.212976049799185	27.931975625333173
4	11.771463566579028	16.980280534400084	36.94308310292345	34.30517279609744
5	13.80610489294671	37.52034466540624	33.65206013966057	15.021490301986484
6	33.2614424337317	36.76584473315444	17.120411977391925	12.852300855721932
7	29.410779799423004	30.881846734748486	21.679487693895474	18.027885771933036
8	27.26852009679357	34.013297802534964	19.728342046584846	18.98984005408662
9	27.49943885037941	14.315439699947868	18.657199127261492	39.52792232241123
10	15.412741630150315	27.00607139355452	32.09946526484097	25.481721711454192
11	37.01662792399094	20.996287642117238	23.285881726480227	18.701202707411593
12	25.017527791051837	23.565466450420182	29.289753392125345	22.127252366402633
13	29.405429376920395	19.72706799467229	25.473965568995503	25.39353705941181
14	23.264146680009038	20.423754047435814	25.31944310782734	30.992656164727812
15	24.71067980027471	28.175221502175273	22.283802141517032	24.830296556032984
16	25.358624330643337	25.8029413982955	24.435930311486537	24.402503959574627
17	23.649906946309425	26.356895973847827	25.637506022319005	24.355691057523742
18	24.61365899661302	24.874613583576213	27.02162121883591	23.490106200974854
19	25.568270094332817	25.075744708580096	25.440688944244112	23.91529625284297
20	25.56112024033163	24.7176184771535	25.24332883928305	24.47793244323182
21	26.578883221288617	24.505000078437337	24.4339237854582	24.482192914815847
22	25.422806894754117	25.004615729924893	25.030319010290963	24.542258365030023
23	23.77820201004843	24.683158226904865	25.82454281564862	25.71409694739808
24	24.784139270708856	25.30954795123498	25.39788032471801	24.508432453338152
25	24.754710082295777	24.868353610874543	25.60370296290732	24.773233343922364
26	24.178467703908982	25.761723882125686	26.053751850440655	24.00605656352468
27	25.000135756844404	25.248570782110264	25.026261407345952	24.725032053699376
28	23.974848201359197	25.28076752626817	25.737965996259753	25.00641827611288
29	24.31369697832689	25.020024116427376	25.647129665468288	25.019149239777445
30	24.127408588211548	25.266799668029567	26.04607341783837	24.559718325920514
31	24.992661594996672	24.866287171321662	24.982283747838828	25.158767485842837
32	24.37729146032902	25.38798516812565	24.90378619462747	25.330937176917857
33	23.945826431109648	25.01510670629154	25.80062526529126	25.238441597307553
34	24.750562108247586	25.01673578695004	26.070871645639958	24.161830459162417
35	25.479998057170473	25.090195257384185	25.58082004903533	23.848986636410014
36	24.237069851056773	25.558736955664568	25.356519776888355	24.8476734163903
37	25.19174128509726	25.39613057141814	24.944694220051993	24.467433923432605
38	24.50198385882262	25.350493792597455	25.482358861236076	24.66516348734385
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	228.0
1	228.0
2	228.0
3	228.0
4	731.5
5	1235.0
6	1235.0
7	1471.5
8	1708.0
9	1757.0
10	1806.0
11	1806.0
12	2434.5
13	3063.0
14	4310.0
15	5557.0
16	5557.0
17	8446.0
18	11335.0
19	11335.0
20	14477.5
21	17620.0
22	21437.5
23	25255.0
24	25255.0
25	31975.0
26	38695.0
27	38695.0
28	50254.5
29	61814.0
30	77685.5
31	93557.0
32	93557.0
33	119279.0
34	145001.0
35	145001.0
36	157743.5
37	170486.0
38	189393.5
39	208301.0
40	208301.0
41	214892.5
42	221484.0
43	242629.0
44	263774.0
45	263774.0
46	266587.5
47	269401.0
48	269401.0
49	285563.5
50	301726.0
51	299653.5
52	297581.0
53	297581.0
54	282229.5
55	266878.0
56	266878.0
57	256250.0
58	245622.0
59	219952.5
60	194283.0
61	194283.0
62	184052.5
63	173822.0
64	142110.0
65	110398.0
66	110398.0
67	92010.5
68	73623.0
69	73623.0
70	59455.5
71	45288.0
72	35143.0
73	24998.0
74	24998.0
75	18457.5
76	11917.0
77	11917.0
78	12104.0
79	12291.0
80	9461.5
81	6632.0
82	6632.0
83	6350.0
84	6068.0
85	6068.0
86	3830.0
87	1592.0
88	1361.5
89	1131.0
90	1131.0
91	664.5
92	198.0
93	135.0
94	72.0
95	72.0
96	57.0
97	42.0
98	42.0
99	42.5
100	43.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008085066971807553
2	0.0
3	0.0
4	2.4134528274052396E-4
5	6.033632068513099E-5
6	2.4134528274052396E-4
7	4.826905654810479E-4
8	0.0021117712239795846
9	0.0038916926841909487
10	8.447084895918338E-4
11	0.004464887730699693
12	5.128587258236134E-4
13	2.4134528274052396E-4
14	0.0
15	0.0
16	9.050448102769648E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.5084080171282746E-4
22	3.0168160342565495E-5
23	2.1117712239795844E-4
24	0.0
25	6.033632068513099E-5
26	6.033632068513099E-5
27	9.050448102769648E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	3.0168160342565495E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	3314753.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.88040744617247
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.59597886947891	35.71685862116722
2	14.855293384839069	14.225549999970502
3	4.815059626517297	6.916410503857898
4	2.0141047034717205	3.857446153659134
5	1.0595733691185951	2.5366402316256016
6	0.6214957757671865	1.7854482581884756
7	0.39706449324432413	1.3308126803262366
8	0.27460718800953393	1.0518643239635337
9	0.19369298352140893	0.8346689073422847
>10	1.0002180310155417	8.63887480898021
>50	0.07389048464867538	2.4630364799921174
>100	0.07557374100076274	7.8228178712962055
>500	0.014512008122186216	4.856027351708981
>1k	0.008871970030510556	7.785160863554593
>5k	6.337121450364682E-5	0.17838294436694424
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5879	0.17735861465394256	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4172	0.12586156494918324	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	4157	0.12540904254404475	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	3975	0.11991843736169783	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3955	0.11931507415484652	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3832	0.11560439043271098	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	3760	0.11343228288804627	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3697	0.11153168878646463	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3630	0.10951042204351273	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	3410	0.10287342676814834	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	3328	0.10039963762005796	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	6.033632068513099E-5
7	0.0	0.0	0.0	0.0	6.033632068513099E-5
8	0.0	0.0	0.0	0.0	6.033632068513099E-5
9	0.0	0.0	0.0	0.0	6.033632068513099E-5
10	0.0	3.0168160342565495E-5	0.0	6.033632068513099E-5	6.033632068513099E-5
11	0.0	3.0168160342565495E-5	0.0	6.033632068513099E-5	6.033632068513099E-5
12	0.0	3.0168160342565495E-5	0.0	6.033632068513099E-5	3.921860844533514E-4
13	0.0	3.0168160342565495E-5	0.0	6.033632068513099E-5	4.2235424479591694E-4
14	0.0	3.0168160342565495E-5	0.0	9.050448102769649E-5	4.2235424479591694E-4
15	0.0	3.0168160342565495E-5	0.0	1.2067264137026198E-4	5.128587258236134E-4
16	0.0	6.033632068513099E-5	0.0	1.5084080171282746E-4	5.430268861661789E-4
17	0.0	6.033632068513099E-5	0.0	1.5084080171282746E-4	5.430268861661789E-4
18	0.0	6.033632068513099E-5	0.0	1.8100896205539298E-4	5.731950465087444E-4
19	0.0	6.033632068513099E-5	0.0	2.1117712239795847E-4	6.636995275364409E-4
20	0.0	6.033632068513099E-5	0.0	2.1117712239795847E-4	7.843721689067028E-4
21	0.0	6.033632068513099E-5	0.0	3.3184976376822044E-4	7.843721689067028E-4
22	0.0	6.033632068513099E-5	0.0	4.525224051384824E-4	7.843721689067028E-4
23	0.0	6.033632068513099E-5	0.0	7.542040085641373E-4	7.843721689067028E-4
24	0.0	6.033632068513099E-5	0.0	0.0013877353757580128	7.843721689067028E-4
25	0.0	6.033632068513099E-5	0.0	0.001779921460211364	7.843721689067028E-4
26	0.0	6.033632068513099E-5	0.0	0.002383284667062674	8.447084895918339E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTTT	825	0.0	16.290388	29
CATCGTT	835	0.0	16.095293	28
CGTTTAT	875	0.0	15.908061	31
CGGCATC	880	0.0	15.635862	25
TCTAGCG	855	0.0	15.157409	28
CTAGCGG	870	0.0	15.079977	29
ACCGTCG	840	0.0	14.8584585	8
GTATCAA	5470	0.0	14.655115	1
TCGTTTA	920	0.0	14.608227	30
GTTTTCG	1055	0.0	14.558774	28
TACCGTC	825	0.0	14.546744	7
TAGCGGC	900	0.0	14.399539	30
GTATAGA	460	0.0	14.261488	1
TTAGGAC	1225	0.0	14.237351	3
TTTTCGG	1070	0.0	14.205152	29
ATTTAGA	960	0.0	14.167281	1
GTCCTAA	1135	0.0	14.0975275	1
GGCATCG	980	0.0	13.877106	26
ATACCGT	880	0.0	13.8194065	6
TGCACCG	220	1.8189894E-12	13.819198	5
>>END_MODULE