FastQCFastQC Report
Thu 2 Feb 2017
SRR4062736_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062736_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197008
Sequences flagged as poor quality0
Sequence length38
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA54332.7577560302119712No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT46262.3481279948022413No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC42352.1496588971006254No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT31701.6090717128238448No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG20081.019247949321855No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT18210.924327946073256No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA18080.9177292292698773No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14640.7431170307804759No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11930.6055591651100463No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG10650.5405871842767807No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC9600.48728985624949245No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA9070.4603873954357183No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC8760.4446519938276618No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA8560.4345001218224641No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA7810.3964306018029724No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG7780.3949078210021928No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA7720.39186225940063346No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA7020.3563307073824413No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6080.30861690895801186No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT5960.3025257857548932No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5860.2974498497522943No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA5300.2690246081377406No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT5270.26750182733696093No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC5140.2609031105335824No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC4810.24415252172500607No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4340.22029562251279136No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT4070.20659059530577437No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3900.1979615041013563No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC3660.18577925769511897No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT3640.1847640704945992No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA3430.17410460488914156No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC3300.16750588808576303No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT3270.16598310728498333No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG3150.1598919840818647No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTA3080.1563388288800455No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTG3080.1563388288800455No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA3060.1553236416795257No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA3020.15329326727848616No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA2970.15075529927718673No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2600.13197433606757086No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG2530.12842118086575166No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTT2510.12740599366523186No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA2410.12233005766263298No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2380.12080727686185333No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA2340.11877690246081378No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA2340.11877690246081378No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA2310.1172541216600341No Hit
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA2250.1142085600584748No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA2220.11268577925769512No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT2220.11268577925769512No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAATC2200.11167059205717533No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2040.10354909445301713No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG1980.1005035328514578No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGAC200.003746320531.9994939
TGGGATG401.5899316E-423.9935266
CTCCATG350.002042501722.891651
CATGTAA851.0433723E-820.70555332
GTTATGA400.004431646320.0301931
GAAAGCA450.00882579717.7774968
CAACTGC450.00882579717.77749622
CCTTGTA450.00882579717.7774962
AAAAAGT1001.2805704E-616.0241551
CTTACAT600.002432533315.99974728
ATGGGAT600.00243666115.9956865
GCTTACA650.00414576114.76899727
AACTGCT650.00414576114.76899723
AAAAGTA1103.506946E-614.5452242
AAGTACT1503.1272066E-813.8629274
AGTACTC1402.0327388E-713.71058755
ATGGGTG3350.013.369535
TGGGTGG2650.013.2794376
GGGGTGG6550.013.1906317
TGGGGTG6550.013.1872836
Produced by FastQC (version 0.11.5)