##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062736_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	197008
Sequences flagged as poor quality	0
Sequence length	38
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.383502192804354	32.0	32.0	32.0	32.0	32.0
2	30.310251360350847	32.0	32.0	32.0	21.0	32.0
3	30.51232437261431	32.0	32.0	32.0	32.0	32.0
4	30.368330220092584	32.0	32.0	32.0	21.0	32.0
5	30.572662023877204	32.0	32.0	32.0	32.0	32.0
6	34.02174023389913	36.0	36.0	36.0	32.0	36.0
7	33.656389588240074	36.0	36.0	36.0	21.0	36.0
8	33.583747868106876	36.0	36.0	36.0	21.0	36.0
9	33.52389750670024	36.0	36.0	36.0	21.0	36.0
10	33.531470803216116	36.0	36.0	36.0	21.0	36.0
11	33.89906501258832	36.0	36.0	36.0	32.0	36.0
12	33.72885872654918	36.0	36.0	36.0	32.0	36.0
13	33.75979148054901	36.0	36.0	36.0	32.0	36.0
14	33.66232335742711	36.0	36.0	36.0	27.0	36.0
15	33.74672094534232	36.0	36.0	36.0	32.0	36.0
16	33.64942540404451	36.0	36.0	36.0	27.0	36.0
17	33.58562596442784	36.0	36.0	36.0	27.0	36.0
18	33.65209027044587	36.0	36.0	36.0	27.0	36.0
19	33.59677271988955	36.0	36.0	36.0	27.0	36.0
20	33.39325814180135	36.0	36.0	36.0	21.0	36.0
21	33.24685291967839	36.0	36.0	36.0	21.0	36.0
22	33.2505786567043	36.0	36.0	36.0	21.0	36.0
23	33.19685495005279	36.0	36.0	36.0	21.0	36.0
24	33.21343295703728	36.0	36.0	36.0	21.0	36.0
25	33.118776902460816	36.0	36.0	36.0	21.0	36.0
26	33.076245634695034	36.0	36.0	36.0	21.0	36.0
27	33.01051733939738	36.0	36.0	36.0	21.0	36.0
28	32.94247949321855	36.0	36.0	36.0	14.0	36.0
29	32.916079550069036	36.0	36.0	36.0	14.0	36.0
30	32.73596503695281	36.0	36.0	36.0	14.0	36.0
31	32.73912734508243	36.0	36.0	36.0	14.0	36.0
32	32.624639608543816	36.0	36.0	36.0	14.0	36.0
33	32.46509786404613	36.0	36.0	36.0	14.0	36.0
34	32.377152196865104	36.0	36.0	36.0	14.0	36.0
35	32.114091813530415	36.0	32.0	36.0	14.0	36.0
36	32.00855802810038	36.0	32.0	36.0	14.0	36.0
37	31.88489807520507	36.0	32.0	36.0	14.0	36.0
38	30.98799033541785	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	5.0
12	2.0
13	3.0
14	147.0
15	228.0
16	266.0
17	392.0
18	552.0
19	683.0
20	816.0
21	1004.0
22	1236.0
23	1533.0
24	1913.0
25	2365.0
26	3237.0
27	4299.0
28	5954.0
29	7931.0
30	10263.0
31	13277.0
32	16479.0
33	21405.0
34	37716.0
35	65298.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.98904070594933	15.450774673661098	13.397686145337726	19.162498475051848
2	12.777563334213681	22.53570485342218	39.18188651646588	25.50484529589826
3	20.26133836922421	33.2104819631852	25.591666412841523	20.936513254749066
4	11.641954691045314	17.348466774617645	32.241133383755574	38.768445150581464
5	11.343759358804508	41.22239311293507	30.77048023674286	16.66336729151756
6	29.288806996705702	32.7389382103174	21.164222591075443	16.808032201901455
7	26.799926906521566	33.29864777064891	21.6427759278811	18.258649394948428
8	32.8697311784293	27.378583610817834	20.51338016730285	19.238305043450012
9	30.644440834889952	15.139994314951677	17.953585641192234	36.26197920896613
10	18.106878908470723	28.492751563388293	29.07699179728742	24.32337773085357
11	34.39389247879474	24.769673558270686	18.53118988035715	22.30524408257742
12	24.52042903771085	26.464601343154026	28.54684541545896	20.46812420367616
13	34.314332710584026	22.54944764844448	24.315652668345383	18.820566972626107
14	28.07030012589855	18.13396011858831	26.76105673557243	27.034683019940708
15	26.756701868399674	22.975223395613323	32.11413484971568	18.153939886271324
16	23.781868394066464	26.7166543135921	27.977175579494574	21.524301712846857
17	21.391218699863934	24.67862147397494	30.249182591742652	23.680977234418474
18	23.658321150097468	22.471978557504872	33.99173570500325	19.877964587394413
19	21.462401835616856	30.271941357727002	30.557741216603805	17.707915590052338
20	23.42086694315984	20.089750293160602	37.22098979141179	19.26839297226777
21	25.228056104085972	24.7610296917118	26.748430131325797	23.262484072876426
22	22.21331032032083	26.260723894613943	28.648662368648154	22.877303416417078
23	20.476582092128375	27.07298995867727	28.448214593930533	24.002213355263827
24	19.358408764386052	30.04076576690916	29.166053234101096	21.434772234603688
25	24.630654414377823	24.484946946235468	27.486926943189317	23.397471696197393
26	19.54347450829077	24.868251373333468	30.501710955190237	25.086563163185527
27	23.62921034596543	28.24174429525091	30.109399192831944	18.019646165951723
28	21.9726889689832	24.521549317224224	30.233006751611757	23.272754962180823
29	18.874753792109164	29.062684021361708	32.91164944057506	19.15091274595407
30	23.80292485824945	25.854183481302123	31.42217552195167	18.92071613849676
31	18.60218383394334	28.522333280877998	30.061474265583044	22.81400861959562
32	18.24365827533517	30.145338064561983	30.37783835645645	21.233165303646395
33	18.975267528986535	28.885008223851198	29.463723678599713	22.67600056856255
34	17.70281246827089	30.79703523200325	31.482383998375468	20.01776830135039
35	21.21527495735521	29.03602469336366	29.99553245065389	19.753167898627243
36	20.734400462998217	31.46560258304269	28.921650751102923	18.87834620285617
37	21.799740080412622	31.43656337570564	29.141960768387282	17.621735775494457
38	19.614203406177822	33.26429604812305	26.33873956191782	20.782760983781316
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	330.0
1	323.0
2	316.0
3	316.0
4	812.5
5	1309.0
6	1309.0
7	1554.0
8	1799.0
9	1718.5
10	1638.0
11	1638.0
12	2116.5
13	2595.0
14	2871.5
15	3148.0
16	3148.0
17	4289.0
18	5430.0
19	5430.0
20	5880.5
21	6331.0
22	4957.5
23	3584.0
24	3584.0
25	2922.0
26	2260.0
27	2260.0
28	3008.5
29	3757.0
30	4860.0
31	5963.0
32	5963.0
33	5217.0
34	4471.0
35	4471.0
36	4880.0
37	5289.0
38	6510.0
39	7731.0
40	7731.0
41	9262.5
42	10794.0
43	13422.0
44	16050.0
45	16050.0
46	25310.0
47	34570.0
48	34570.0
49	27979.5
50	21389.0
51	16212.5
52	11036.0
53	11036.0
54	9700.0
55	8364.0
56	8364.0
57	8434.5
58	8505.0
59	8066.0
60	7627.0
61	7627.0
62	7009.0
63	6391.0
64	5940.5
65	5490.0
66	5490.0
67	4777.0
68	4064.0
69	4064.0
70	3460.5
71	2857.0
72	2322.5
73	1788.0
74	1788.0
75	1421.0
76	1054.0
77	1054.0
78	819.5
79	585.0
80	398.5
81	212.0
82	212.0
83	148.0
84	84.0
85	84.0
86	58.0
87	32.0
88	27.0
89	22.0
90	22.0
91	19.5
92	17.0
93	16.5
94	16.0
95	16.0
96	15.5
97	15.0
98	15.0
99	55.0
100	95.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14212620807276863
2	0.058880857630147
3	0.011167059205717535
4	0.0025379680012994395
5	5.075936002598879E-4
6	5.075936002598879E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0025379680012994395
12	0.0045683424023389915
13	0.016242995208316412
14	0.01218224640623731
15	0.02436449281247462
16	0.013197433606757085
17	0.02233411841143507
18	0.008121497604158206
19	0.008629091204418094
20	0.008629091204418094
21	0.008629091204418094
22	0.009136684804677983
23	0.011167059205717535
24	0.01472021440753675
25	0.01928855680987574
26	0.02131893121091529
27	0.011674652805977423
28	0.009136684804677983
29	0.010151872005197758
30	0.003553155201819215
31	0.0076139040038983195
32	0.00964427840493787
33	0.010151872005197758
34	0.01421262080727686
35	0.016242995208316412
36	0.015735401608056525
37	0.01218224640623731
38	0.006598716803378543
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	197008.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.90443027694307
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.55816839362164	29.679505400795907
2	13.549668052367068	11.084829042475434
3	5.652416702860333	6.9362665475513685
4	2.821865111373084	4.61707138796394
5	1.5834212322392505	3.2384471696580848
6	0.9530309610969784	2.3389913099975637
7	0.6552087857541726	1.8760659465605456
8	0.4616243717813489	1.5105985543734264
9	0.2928584724204256	1.078128806952002
>10	1.254575913631569	8.74126939007553
>50	0.09679220698641186	2.6806018029724683
>100	0.09058757833343674	7.530658653455697
>500	0.017372960228330334	5.072382847397059
>1k	0.011168331575355215	10.857427109559001
>5k	0.0012409257305950239	2.7577560302119712
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	5433	2.7577560302119712	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4626	2.3481279948022413	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	4235	2.1496588971006254	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3170	1.6090717128238448	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	2008	1.019247949321855	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1821	0.924327946073256	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1808	0.9177292292698773	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1464	0.7431170307804759	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	1193	0.6055591651100463	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	1065	0.5405871842767807	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	960	0.48728985624949245	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	907	0.4603873954357183	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	876	0.4446519938276618	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	856	0.4345001218224641	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	781	0.3964306018029724	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG	778	0.3949078210021928	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	772	0.39186225940063346	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	702	0.3563307073824413	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	608	0.30861690895801186	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	596	0.3025257857548932	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	586	0.2974498497522943	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	530	0.2690246081377406	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	527	0.26750182733696093	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	514	0.2609031105335824	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC	481	0.24415252172500607	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	434	0.22029562251279136	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	407	0.20659059530577437	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	390	0.1979615041013563	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	366	0.18577925769511897	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT	364	0.1847640704945992	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	343	0.17410460488914156	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	330	0.16750588808576303	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	327	0.16598310728498333	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	315	0.1598919840818647	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTA	308	0.1563388288800455	No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTG	308	0.1563388288800455	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	306	0.1553236416795257	No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	302	0.15329326727848616	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA	297	0.15075529927718673	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	260	0.13197433606757086	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	253	0.12842118086575166	No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTT	251	0.12740599366523186	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	241	0.12233005766263298	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	238	0.12080727686185333	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA	234	0.11877690246081378	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA	234	0.11877690246081378	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	231	0.1172541216600341	No Hit
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	225	0.1142085600584748	No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA	222	0.11268577925769512	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT	222	0.11268577925769512	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAATC	220	0.11167059205717533	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	204	0.10354909445301713	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG	198	0.1005035328514578	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.075936002598879E-4	0.0
3	0.0	0.0	0.0	5.075936002598879E-4	0.0
4	0.0	0.0	0.0	5.075936002598879E-4	0.0
5	0.0	0.0	0.0	0.0010151872005197758	0.0
6	0.0	0.0	0.0	0.0010151872005197758	0.0
7	0.0	0.0	0.0	0.0010151872005197758	0.0
8	0.0	0.0	0.0	0.0010151872005197758	0.0
9	0.0	0.0	0.0	0.0015227808007796638	0.0
10	0.0	0.0	0.0	0.0015227808007796638	0.0
11	0.0	0.0	0.0	0.0015227808007796638	0.0
12	0.0	0.0	0.0	0.0020303744010395515	0.0
13	0.0	0.0	0.0	0.0020303744010395515	0.0
14	0.0	0.0	0.0	0.0020303744010395515	0.0
15	0.0	0.0	0.0	0.0020303744010395515	0.0
16	0.0	0.0	0.0	0.0020303744010395515	0.0
17	0.0	0.0	0.0	0.0020303744010395515	0.0
18	0.0	0.0	0.0	0.0025379680012994395	0.0
19	0.0	0.0	0.0	0.0025379680012994395	0.0
20	0.0	0.0	0.0	0.0025379680012994395	0.0
21	0.0	0.0	0.0	0.0030455616015593275	0.0
22	0.0	0.0	0.0	0.009136684804677983	0.0
23	0.0	0.0	0.0	0.01979615041013563	0.0
24	0.0	0.0	0.0	0.03147080321611305	0.0
25	0.0	0.0	0.0	0.03806952001949159	0.0
26	0.0	0.0	0.0	0.04822139202468935	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAAGAC	20	0.0037463205	31.999493	9
TGGGATG	40	1.5899316E-4	23.993526	6
CTCCATG	35	0.0020425017	22.89165	1
CATGTAA	85	1.0433723E-8	20.705553	32
GTTATGA	40	0.0044316463	20.030193	1
GAAAGCA	45	0.008825797	17.777496	8
CAACTGC	45	0.008825797	17.777496	22
CCTTGTA	45	0.008825797	17.777496	2
AAAAAGT	100	1.2805704E-6	16.024155	1
CTTACAT	60	0.0024325333	15.999747	28
ATGGGAT	60	0.002436661	15.995686	5
GCTTACA	65	0.004145761	14.768997	27
AACTGCT	65	0.004145761	14.768997	23
AAAAGTA	110	3.506946E-6	14.545224	2
AAGTACT	150	3.1272066E-8	13.862927	4
AGTACTC	140	2.0327388E-7	13.7105875	5
ATGGGTG	335	0.0	13.36953	5
TGGGTGG	265	0.0	13.279437	6
GGGGTGG	655	0.0	13.190631	7
TGGGGTG	655	0.0	13.187283	6
>>END_MODULE