FastQCFastQC Report
Thu 2 Feb 2017
SRR4062736_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062736_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197008
Sequences flagged as poor quality0
Sequence length38
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT55152.799378705433282No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA53212.7009055469828636No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC51222.599894420531146No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT39772.0186997482335745No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT23681.2019816454154146No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG21751.1040160805652561No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17990.9131608868675385No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA17180.8720458052464874No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC14150.7182449443677413No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG12390.6289084707220011No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG12280.6233249411191424No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC11370.5771339234954925No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA11230.5700276130918541No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA9790.4969341346544303No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG9150.46444814423779746No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA8180.4152115650125883No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7790.3954154146024527No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT7520.3817103873954357No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7090.3598838625842605No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA6890.3497319905790628No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA6580.33399658897100626No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6540.33196621456996667No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT6540.33196621456996667No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC6490.32942824656866726No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC6250.31724600016242993No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA6050.3070941281572322No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT5110.25938032973280273No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT4890.2482132705270852No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC4730.24009177292292702No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA4460.22638674571591003No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT4400.22334118411435067No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG4200.21318931210915293No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC4090.20760578250629416No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTG4020.20405262730447496No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3790.19237797449849753No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA3470.1761349792901811No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA3370.17105904328758223No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTT3360.17055144968732233No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT3250.16496792008446357No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG3150.1598919840818647No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3050.1548160480792658No Hit
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA2800.14212620807276863No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA2800.14212620807276863No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG2770.14060342727198893No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA2770.14060342727198893No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA2500.12689840006497197No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA2380.12080727686185333No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA2320.117761715260294No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2260.11471615365873467No Hit
ACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAG2230.11319337285795501No Hit
GCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC2200.11167059205717533No Hit
GATACCACTGCTTCCATGTACTCTGCGTTGATACCACT2140.10862503045561603No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2110.10710224965483635No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT2090.10608706245431657No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA2080.10557946885405668No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2080.10557946885405668No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA2060.10456428165353691No Hit
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC2060.10456428165353691No Hit
TGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG2040.10354909445301713No Hit
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCA2030.10304150085275726No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA1980.1005035328514578No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAGT252.8864102E-432.04
ACTGATT502.7824426E-522.424
CATTGTT400.004470532820.07
CATGTAA400.004470532820.032
ATACATG801.2522287E-720.01
CACCACA400.004470532820.02
ACATTGT507.167022E-419.26
CCACTGA507.167022E-419.222
AAAAGTA904.3214823E-717.7777792
GCACCGA450.00882499917.7777796
GCCAGCA450.00882499917.7777792
CCAGCAC854.941814E-616.9411773
AAAAAGT957.6131437E-716.8421061
AAGTACT1304.7184585E-916.04
TGGGTGT600.002432279316.06
TGTACTA805.583042E-516.032
AGTACTC1101.9943946E-716.05
CACTGAT756.2118785E-414.93333323
AAAGTAC1205.3504846E-714.6666673
TACTCTG24250.012.87
Produced by FastQC (version 0.11.5)