##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062736_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	197008
Sequences flagged as poor quality	0
Sequence length	38
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.389755745959555	32.0	32.0	32.0	27.0	32.0
2	31.23674165516121	32.0	32.0	32.0	32.0	32.0
3	31.34683363112158	32.0	32.0	32.0	32.0	32.0
4	31.502720701697392	32.0	32.0	32.0	32.0	32.0
5	31.36761451311622	32.0	32.0	32.0	32.0	32.0
6	34.81946926013157	36.0	36.0	36.0	32.0	36.0
7	34.89114655242427	36.0	36.0	36.0	36.0	36.0
8	34.88891821651912	36.0	36.0	36.0	36.0	36.0
9	34.919246934134655	36.0	36.0	36.0	32.0	36.0
10	34.80509928530821	36.0	36.0	36.0	32.0	36.0
11	34.96345833671729	36.0	36.0	36.0	36.0	36.0
12	34.87101539023796	36.0	36.0	36.0	32.0	36.0
13	34.85089437992366	36.0	36.0	36.0	32.0	36.0
14	34.782922520912855	36.0	36.0	36.0	32.0	36.0
15	34.77110574189881	36.0	36.0	36.0	32.0	36.0
16	34.72244274344189	36.0	36.0	36.0	32.0	36.0
17	34.691032851457805	36.0	36.0	36.0	32.0	36.0
18	34.64184195565662	36.0	36.0	36.0	32.0	36.0
19	34.624791886623896	36.0	36.0	36.0	32.0	36.0
20	34.541673434581334	36.0	36.0	36.0	32.0	36.0
21	34.43005867782019	36.0	36.0	36.0	32.0	36.0
22	34.39190794282466	36.0	36.0	36.0	32.0	36.0
23	34.25815195322017	36.0	36.0	36.0	32.0	36.0
24	34.29977462844148	36.0	36.0	36.0	32.0	36.0
25	34.21728051652725	36.0	36.0	36.0	32.0	36.0
26	34.0789003492244	36.0	36.0	36.0	32.0	36.0
27	34.01617193210428	36.0	36.0	36.0	32.0	36.0
28	33.88658328595793	36.0	36.0	36.0	32.0	36.0
29	33.83194591082596	36.0	36.0	36.0	32.0	36.0
30	33.74287338585235	36.0	36.0	36.0	32.0	36.0
31	33.621746325022336	36.0	36.0	36.0	27.0	36.0
32	33.55844940306993	36.0	36.0	36.0	27.0	36.0
33	33.35203646552424	36.0	36.0	36.0	21.0	36.0
34	33.20894075367498	36.0	36.0	36.0	14.0	36.0
35	32.95845853975473	36.0	36.0	36.0	14.0	36.0
36	32.85219889547633	36.0	36.0	36.0	14.0	36.0
37	32.658623000081214	36.0	36.0	36.0	14.0	36.0
38	32.18103325753269	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	5.0
21	4.0
22	16.0
23	72.0
24	234.0
25	558.0
26	1219.0
27	2201.0
28	3940.0
29	6177.0
30	9147.0
31	12190.0
32	16254.0
33	22711.0
34	43544.0
35	78736.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.49668262324042	16.658459945277244	14.043646220931707	19.80121121055063
2	12.736538617721108	23.377730853569396	37.36142694712905	26.524303581580444
3	20.140806464712092	32.46771704702347	26.264923251847637	21.126553236416797
4	11.66106616042151	17.641594672243485	32.05486127326071	38.64247789407429
5	11.175688296921953	40.781085032079915	31.107366198326968	16.93586047267116
6	30.026191829773406	32.951453748071145	19.942844960610735	17.07950946154471
7	25.73499553317632	33.345346381872815	22.00672053926744	18.912937545683427
8	32.682575215354234	27.482601611159335	20.69177313820742	19.143050035279007
9	29.875482865569207	14.31363292572119	18.508535489667565	37.30234871904203
10	18.115184309107338	28.03467914682802	29.726505791701776	24.123630752362875
11	34.42996593856759	24.098336522891213	18.766783250506354	22.704914288034843
12	24.05841387151791	26.61313246162592	28.999329976447658	20.32912369040851
13	34.878786648257936	21.757492081539837	24.848229513522295	18.515491756679932
14	27.543043937302038	17.954600828392756	27.490761796475272	27.011593437829934
15	27.108036221879317	22.680297246812312	32.2281328677008	17.98353366360757
16	23.77365386177211	25.600483229107446	28.8785226995858	21.747340209534638
17	21.127060830017054	24.04521643791115	31.39161861447251	23.436104117599285
18	23.816799317794203	21.128076017217577	35.22547307723544	19.82965158775278
19	21.448875172581825	29.12419800211159	31.843884512304072	17.583042313002515
20	23.178754162267523	19.376370502720704	38.38828880045481	19.05658653455697
21	24.658897100625353	23.931007877852675	28.321692520100704	23.088402501421264
22	21.933627060830016	24.690367903841466	30.46830585559977	22.90769917972874
23	20.289531389588237	25.226894339316168	30.10385365061317	24.379720620482416
24	19.24033541785105	28.75466986112239	30.61956874847722	21.38542597254934
25	24.53605944936246	23.0107406805815	28.908978315601395	23.54422155445464
26	19.599711686835054	23.212255339884674	32.3433566149598	24.844676358320473
27	24.058921465118168	26.421262080727686	31.71140258263624	17.80841387151791
28	21.947839681637294	22.55796718914968	31.951494355559163	23.54269877365386
29	19.098716803378544	26.785714285714285	34.8742183058556	19.241350605051572
30	23.87821814342565	23.984305205879963	33.2214935434094	18.915983107284983
31	18.583509299114755	25.973056931698206	31.85302119710875	23.590412572078293
32	18.658633151953218	27.39685698042719	32.27533907252497	21.669170795094615
33	19.218001299439617	26.74053845529116	31.30025176642573	22.7412084788435
34	18.056119548444734	28.197332088037037	33.6859416876472	20.060606675871032
35	22.020425566474458	26.953220173800048	31.39161861447251	19.634735645252984
36	21.212844148460977	28.56787541622675	31.132745878339968	19.086534556972307
37	22.454418094696663	28.87395435718346	30.909912287825875	17.761715260293997
38	20.489523268090636	29.468854056688055	28.354178510517343	21.68744416470397
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	146.0
1	159.5
2	173.0
3	173.0
4	684.5
5	1196.0
6	1196.0
7	1535.5
8	1875.0
9	1916.0
10	1957.0
11	1957.0
12	2277.5
13	2598.0
14	2948.0
15	3298.0
16	3298.0
17	4777.5
18	6257.0
19	6257.0
20	6537.5
21	6818.0
22	5242.5
23	3667.0
24	3667.0
25	2742.5
26	1818.0
27	1818.0
28	2617.5
29	3417.0
30	4452.0
31	5487.0
32	5487.0
33	4826.5
34	4166.0
35	4166.0
36	4367.5
37	4569.0
38	5557.5
39	6546.0
40	6546.0
41	7842.0
42	9138.0
43	11469.5
44	13801.0
45	13801.0
46	25148.0
47	36495.0
48	36495.0
49	30480.5
50	24466.0
51	18279.5
52	12093.0
53	12093.0
54	10365.0
55	8637.0
56	8637.0
57	8458.5
58	8280.0
59	7870.5
60	7461.0
61	7461.0
62	6938.5
63	6416.0
64	5943.0
65	5470.0
66	5470.0
67	4773.5
68	4077.0
69	4077.0
70	3521.5
71	2966.0
72	2372.5
73	1779.0
74	1779.0
75	1424.5
76	1070.0
77	1070.0
78	826.0
79	582.0
80	399.0
81	216.0
82	216.0
83	132.0
84	48.0
85	48.0
86	30.0
87	12.0
88	10.0
89	8.0
90	8.0
91	4.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.5
97	2.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0076139040038983195
2	0.0
3	0.0
4	0.0010151872005197758
5	0.0
6	0.0
7	0.0
8	0.003553155201819215
9	0.003553155201819215
10	0.0010151872005197758
11	0.0055835296028587675
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	197008.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.335580281003814
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.96792579347341	25.059896044830666
2	15.193339293696914	11.041175992853082
3	6.381314260169871	6.956062697961504
4	3.2591081805990165	4.736863477625274
5	1.887293249888243	3.4287947697555428
6	1.1259499329459097	2.454722650856818
7	0.7320071524362987	1.861853325753269
8	0.4945239159588735	1.4375050759360026
9	0.37298837729101475	1.2197474214245108
>10	1.3382878855610192	8.6011735564038
>50	0.1019780956638355	2.651668967757655
>100	0.10756593652212784	8.14840006497198
>500	0.019557443004023248	5.0744132217981
>1k	0.013969602145730889	9.227544059124503
>5k	0.004190880643719267	8.100178672947292
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5515	2.799378705433282	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	5321	2.7009055469828636	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	5122	2.599894420531146	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3977	2.0186997482335745	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2368	1.2019816454154146	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	2175	1.1040160805652561	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1799	0.9131608868675385	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1718	0.8720458052464874	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	1415	0.7182449443677413	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	1239	0.6289084707220011	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	1228	0.6233249411191424	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	1137	0.5771339234954925	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	1123	0.5700276130918541	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	979	0.4969341346544303	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG	915	0.46444814423779746	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	818	0.4152115650125883	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	779	0.3954154146024527	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	752	0.3817103873954357	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	709	0.3598838625842605	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	689	0.3497319905790628	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	658	0.33399658897100626	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	654	0.33196621456996667	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	654	0.33196621456996667	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	649	0.32942824656866726	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC	625	0.31724600016242993	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	605	0.3070941281572322	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	511	0.25938032973280273	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT	489	0.2482132705270852	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	473	0.24009177292292702	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	446	0.22638674571591003	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	440	0.22334118411435067	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	420	0.21318931210915293	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	409	0.20760578250629416	No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTG	402	0.20405262730447496	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	379	0.19237797449849753	No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	347	0.1761349792901811	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	337	0.17105904328758223	No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTT	336	0.17055144968732233	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT	325	0.16496792008446357	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	315	0.1598919840818647	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	305	0.1548160480792658	No Hit
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	280	0.14212620807276863	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA	280	0.14212620807276863	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG	277	0.14060342727198893	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	277	0.14060342727198893	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA	250	0.12689840006497197	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA	238	0.12080727686185333	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	232	0.117761715260294	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	226	0.11471615365873467	No Hit
ACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAG	223	0.11319337285795501	No Hit
GCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC	220	0.11167059205717533	No Hit
GATACCACTGCTTCCATGTACTCTGCGTTGATACCACT	214	0.10862503045561603	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	211	0.10710224965483635	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT	209	0.10608706245431657	No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA	208	0.10557946885405668	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	208	0.10557946885405668	No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA	206	0.10456428165353691	No Hit
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	206	0.10456428165353691	No Hit
TGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	204	0.10354909445301713	No Hit
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCA	203	0.10304150085275726	No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA	198	0.1005035328514578	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.075936002598879E-4	0.0
3	0.0	0.0	0.0	5.075936002598879E-4	0.0
4	0.0	0.0	0.0	5.075936002598879E-4	0.0
5	0.0	0.0	0.0	0.0010151872005197758	0.0
6	0.0	0.0	0.0	0.0010151872005197758	0.0
7	0.0	0.0	0.0	0.0010151872005197758	0.0
8	0.0	0.0	0.0	0.0010151872005197758	0.0
9	0.0	0.0	0.0	0.0015227808007796638	0.0
10	0.0	0.0	0.0	0.0015227808007796638	0.0
11	0.0	0.0	0.0	0.0015227808007796638	0.0
12	0.0	0.0	0.0	0.0020303744010395515	0.0
13	0.0	0.0	0.0	0.0020303744010395515	0.0
14	0.0	0.0	0.0	0.0020303744010395515	0.0
15	0.0	0.0	0.0	0.0020303744010395515	0.0
16	0.0	0.0	0.0	0.0020303744010395515	0.0
17	0.0	0.0	0.0	0.0020303744010395515	0.0
18	0.0	0.0	0.0	0.0025379680012994395	0.0
19	0.0	0.0	0.0	0.0025379680012994395	0.0
20	0.0	0.0	0.0	0.0025379680012994395	0.0
21	0.0	0.0	0.0	0.0030455616015593275	0.0
22	0.0	0.0	0.0	0.007613904003898319	0.0
23	0.0	0.0	0.0	0.018780963209615854	0.0
24	0.0	0.0	0.0	0.030455616015593275	0.0
25	0.0	0.0	0.0	0.03756192641923171	0.0
26	0.0	0.0	0.0	0.047713798424429466	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGAGT	25	2.8864102E-4	32.0	4
ACTGATT	50	2.7824426E-5	22.4	24
CATTGTT	40	0.0044705328	20.0	7
CATGTAA	40	0.0044705328	20.0	32
ATACATG	80	1.2522287E-7	20.0	1
CACCACA	40	0.0044705328	20.0	2
ACATTGT	50	7.167022E-4	19.2	6
CCACTGA	50	7.167022E-4	19.2	22
AAAAGTA	90	4.3214823E-7	17.777779	2
GCACCGA	45	0.008824999	17.777779	6
GCCAGCA	45	0.008824999	17.777779	2
CCAGCAC	85	4.941814E-6	16.941177	3
AAAAAGT	95	7.6131437E-7	16.842106	1
AAGTACT	130	4.7184585E-9	16.0	4
TGGGTGT	60	0.0024322793	16.0	6
TGTACTA	80	5.583042E-5	16.0	32
AGTACTC	110	1.9943946E-7	16.0	5
CACTGAT	75	6.2118785E-4	14.933333	23
AAAGTAC	120	5.3504846E-7	14.666667	3
TACTCTG	2425	0.0	12.8	7
>>END_MODULE