FastQCFastQC Report
Thu 2 Feb 2017
SRR4062735_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062735_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6078519
Sequences flagged as poor quality0
Sequence length38
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCG210790.34677854918278617No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT170730.2808743379760761No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACT137180.22567997237484985No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGT127820.21028148468401597No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCA99290.1633457097033011No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT98040.16128928773604229No Hit
ACTTAGAACTGGTACGGACAAGGGGAATCTGACTGTCT87310.14363696156909272No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCC84020.1382244589512676No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACA81000.1332561434783703No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTA79750.1311997215111115No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCG76310.1255404482572153No Hit
GTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGA73820.12144405569843575No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTT72630.11948634198560538No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGG72250.1188611897075587No Hit
ATTCCATTCCATTCCATTCCATTCCATTCCATTCCATT69300.11400803386482793No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAG66800.10989518993031033No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGC65360.10752619182402819No Hit
CCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTTGAA63980.10525590197217446No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTT63020.10367656990131972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55650.023.603021
GTATCAA190950.022.2135091
AGTCCGT13750.015.7054146
GTCCTAG11100.015.5854621
TGCGCTA8900.015.4612510
GTCTAGG4950.015.2093811
CGGACGG39800.015.19454628
CCTAGAC5650.015.006943
ATCAACG285650.014.7574423
ACTGGTA29250.014.7130218
GCGCTAT9500.014.65317711
AACGCAG287400.014.6214886
CGCTTCA5700.014.59703514
TCAACGC289250.014.5555024
AACTGGT29750.014.3581917
CTGGTAC29050.014.2654759
TACGCCG23950.014.09563223
CGGCCGA28500.014.09059432
TCCTAGA7500.014.0856242
GTCCTAC6150.014.0649281
Produced by FastQC (version 0.11.5)