##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062735_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6078519
Sequences flagged as poor quality	0
Sequence length	38
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.288444603035707	32.0	32.0	32.0	32.0	32.0
2	30.96376946423956	32.0	32.0	32.0	32.0	32.0
3	31.01212515746023	32.0	32.0	32.0	32.0	32.0
4	31.048842489428758	32.0	32.0	32.0	32.0	32.0
5	30.98102185746232	32.0	32.0	32.0	32.0	32.0
6	34.62239617906928	36.0	36.0	36.0	32.0	36.0
7	34.58052364399947	36.0	36.0	36.0	32.0	36.0
8	34.55262721067418	36.0	36.0	36.0	32.0	36.0
9	34.684405691583756	36.0	36.0	36.0	32.0	36.0
10	34.45332029068265	36.0	36.0	36.0	32.0	36.0
11	34.675599434664925	36.0	36.0	36.0	32.0	36.0
12	34.55735155224488	36.0	36.0	36.0	32.0	36.0
13	34.57691750243768	36.0	36.0	36.0	32.0	36.0
14	34.516930357542684	36.0	36.0	36.0	32.0	36.0
15	34.47489594093561	36.0	36.0	36.0	32.0	36.0
16	34.469883042234464	36.0	36.0	36.0	32.0	36.0
17	34.441994999110804	36.0	36.0	36.0	32.0	36.0
18	34.44343219129528	36.0	36.0	36.0	32.0	36.0
19	34.40492791089409	36.0	36.0	36.0	32.0	36.0
20	34.38144521716556	36.0	36.0	36.0	32.0	36.0
21	34.38294656971542	36.0	36.0	36.0	32.0	36.0
22	34.376409122024626	36.0	36.0	36.0	32.0	36.0
23	34.32142796625296	36.0	36.0	36.0	32.0	36.0
24	34.25894333800717	36.0	36.0	36.0	32.0	36.0
25	34.23928509559648	36.0	36.0	36.0	32.0	36.0
26	34.20638530536797	36.0	36.0	36.0	32.0	36.0
27	34.242054026646954	36.0	36.0	36.0	32.0	36.0
28	34.19823545834109	36.0	36.0	36.0	32.0	36.0
29	34.11097522274751	36.0	36.0	36.0	32.0	36.0
30	34.10279724386812	36.0	36.0	36.0	32.0	36.0
31	34.081105775929956	36.0	36.0	36.0	32.0	36.0
32	34.11065902730583	36.0	36.0	36.0	32.0	36.0
33	34.0541974122315	36.0	36.0	36.0	32.0	36.0
34	34.007098110575946	36.0	36.0	36.0	32.0	36.0
35	33.978882356047585	36.0	36.0	36.0	32.0	36.0
36	33.92077905818835	36.0	36.0	36.0	32.0	36.0
37	33.97123444049447	36.0	36.0	36.0	32.0	36.0
38	33.4231802187342	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	2.0
6	3.0
7	8.0
8	6.0
9	15.0
10	50.0
11	49.0
12	41.0
13	39.0
14	594.0
15	1469.0
16	2203.0
17	2899.0
18	3809.0
19	5010.0
20	6235.0
21	8357.0
22	11488.0
23	16135.0
24	23488.0
25	34818.0
26	51445.0
27	75115.0
28	105692.0
29	144120.0
30	196374.0
31	266800.0
32	369083.0
33	561591.0
34	1295094.0
35	2896487.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.062524089411994	21.604109414850477	12.502561301582638	21.830805194154895
2	14.824699569512042	21.352205911279615	41.1148507234084	22.70824379579994
3	16.707627420522233	28.747966564704914	30.682541570763878	23.86186444400898
4	11.34758120439232	15.397094763615366	39.424365389535126	33.83095864245719
5	14.224860446633288	36.96422422706658	33.09147537903684	15.719439947263297
6	29.82198257269138	40.538316487636926	17.20403006923083	12.43567087044086
7	25.883583117395247	33.963563810054325	23.72414192474908	16.428711147801348
8	26.619667148254617	34.53118876986468	20.847422605108957	18.001721476771746
9	26.089897747900554	13.840127820882303	22.142661371067167	37.92731306014998
10	16.21007894058004	27.3846523876959	30.62295344111438	25.782315230609676
11	35.79183424075325	25.410938328239347	21.10591164854733	17.691315782460073
12	22.056823184659557	26.505125392293056	32.318482407437266	19.11956901561012
13	26.332622583737948	25.13297555079529	27.34398870873982	21.19041315672695
14	21.964427179865595	19.65309055430651	27.73163012888381	30.65085213694409
15	25.767071960665255	27.612566582250984	23.38592446688947	23.234436990194286
16	25.20307760196083	30.378016959618442	22.956213620970182	21.462691817450548
17	19.725629235981863	28.329947208038636	28.750235920813537	23.194187635165967
18	21.00976987014363	30.160145056651032	27.31279460930473	21.517290463900608
19	23.94695481109689	24.813020200679066	28.0117530864685	23.228271901755544
20	26.34844128969166	26.033954979182866	25.70230888725316	21.915294843872314
21	26.798401479404816	25.542453916671604	25.813195527564442	21.845949076359137
22	24.21299853407414	26.810846206114768	28.07697180190422	20.89918345790687
23	23.218886358614487	27.922591701515298	27.21309296073859	21.645428979131626
24	23.448822240211662	25.879239309995327	27.021738701206388	23.650199748586623
25	24.295447649649297	26.481322180457806	25.431774638024073	23.79145553186882
26	24.65421291819303	27.473443680462278	25.741624621320387	22.13071878002431
27	21.701702264953656	28.06813008507294	28.640032405604444	21.59013524436896
28	21.27504572913414	28.754535484459005	27.871557646677903	22.09886113972895
29	22.780939450274285	25.760818291920256	27.782789616888	23.675452640917456
30	23.522286176065776	26.740746078453633	26.7502552505272	22.986712494953395
31	24.86099011790565	27.98964819151833	23.822930111308267	23.326431579267755
32	21.271584395038072	26.987942649352973	27.970582341441876	23.769890614167082
33	22.02747867645099	27.85985309036394	28.255294238816507	21.85737399436856
34	22.31853864774042	25.170342138758834	29.364842488229232	23.146276725271512
35	23.580387178725655	25.920275505528128	26.448300344464183	24.051036971282038
36	24.60342924613055	27.42004908513289	25.32591791107153	22.650603757665028
37	22.585738156646908	26.587959967910095	27.28653731109504	23.53976456434796
38	21.873863759382363	28.838325690118616	27.77027184880683	21.51753870169219
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	96.0
1	284.0
2	472.0
3	472.0
4	1290.0
5	2108.0
6	2108.0
7	2940.5
8	3773.0
9	4559.5
10	5346.0
11	5346.0
12	8532.5
13	11719.0
14	17798.5
15	23878.0
16	23878.0
17	35569.0
18	47260.0
19	47260.0
20	62076.5
21	76893.0
22	90123.5
23	103354.0
24	103354.0
25	121911.0
26	140468.0
27	140468.0
28	157559.5
29	174651.0
30	198732.5
31	222814.0
32	222814.0
33	259704.5
34	296595.0
35	296595.0
36	370600.5
37	444606.0
38	490695.5
39	536785.0
40	536785.0
41	536621.0
42	536457.0
43	536753.0
44	537049.0
45	537049.0
46	539780.0
47	542511.0
48	542511.0
49	496988.5
50	451466.0
51	424816.0
52	398166.0
53	398166.0
54	363840.5
55	329515.0
56	329515.0
57	291397.5
58	253280.0
59	246825.5
60	240371.0
61	240371.0
62	214013.0
63	187655.0
64	168170.5
65	148686.0
66	148686.0
67	133706.5
68	118727.0
69	118727.0
70	103827.0
71	88927.0
72	79184.0
73	69441.0
74	69441.0
75	54018.0
76	38595.0
77	38595.0
78	29518.5
79	20442.0
80	16512.0
81	12582.0
82	12582.0
83	8801.0
84	5020.0
85	5020.0
86	3893.0
87	2766.0
88	2069.5
89	1373.0
90	1373.0
91	1110.0
92	847.0
93	782.0
94	717.0
95	717.0
96	716.0
97	715.0
98	715.0
99	1554.0
100	2393.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12152631257712611
2	0.05461856745039376
3	0.012338531803552806
4	0.003027053135804955
5	3.2902751476140816E-4
6	2.1386788459491532E-4
7	3.290275147614081E-5
8	2.7967338754719695E-4
9	2.1386788459491532E-4
10	3.783816419756194E-4
11	0.001859005458401956
12	0.0025499632394009133
13	0.014773335412787228
14	0.00927857591627171
15	0.02383804344446402
16	0.011022421744507174
17	0.020860344435873278
18	0.00562637050242008
19	0.007501827336560106
20	0.0058073356355388545
21	0.006564098919490093
22	0.007386667706393614
23	0.00931147866774785
24	0.014460759273763888
25	0.017158784894807436
26	0.01895198485025711
27	0.009179867661843288
28	0.005544113623729727
29	0.01131854650779244
30	0.0029118935056384626
31	0.005823787011276925
32	0.006597001670966233
33	0.008176333741820993
34	0.012354983179290875
35	0.015859126211499874
36	0.015053008800334423
37	0.009788568564151893
38	0.005938946641443417
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	6078519.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.019621669445513
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.356171627602606	15.100885795387384
2	13.594478823424502	6.802574339107396
3	7.015217307452211	5.265542488842016
4	4.397431647512159	4.400883045520028
5	3.0118745334631254	3.767798067154255
6	2.1322898017359084	3.2009450477429646
7	1.5508918004257757	2.716190826782867
8	1.213319413236487	2.428543414669642
9	0.9438286429883074	2.1252812011518247
>10	5.2440960023737295	22.743126205231174
>50	0.27040329870910657	4.739378239898883
>100	0.2262593596524509	11.478827868452413
>500	0.026677393751860737	4.616075044540352
>1k	0.015347725897842066	6.987427183169205
>5k	0.0014491415010820063	2.5610277049064845
>10k+	2.634802729240012E-4	1.065493527443172
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCG	21079	0.34677854918278617	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	17073	0.2808743379760761	No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACT	13718	0.22567997237484985	No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGT	12782	0.21028148468401597	No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCA	9929	0.1633457097033011	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	9804	0.16128928773604229	No Hit
ACTTAGAACTGGTACGGACAAGGGGAATCTGACTGTCT	8731	0.14363696156909272	No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCC	8402	0.1382244589512676	No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACA	8100	0.1332561434783703	No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTA	7975	0.1311997215111115	No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCG	7631	0.1255404482572153	No Hit
GTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGA	7382	0.12144405569843575	No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTT	7263	0.11948634198560538	No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGG	7225	0.1188611897075587	No Hit
ATTCCATTCCATTCCATTCCATTCCATTCCATTCCATT	6930	0.11400803386482793	No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAG	6680	0.10989518993031033	No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGC	6536	0.10752619182402819	No Hit
CCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTTGAA	6398	0.10525590197217446	No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTT	6302	0.10367656990131972	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.290275147614081E-5	0.0
2	0.0	0.0	0.0	3.290275147614081E-5	0.0
3	0.0	0.0	0.0	3.290275147614081E-5	0.0
4	0.0	0.0	0.0	3.290275147614081E-5	0.0
5	0.0	0.0	0.0	3.290275147614081E-5	0.0
6	0.0	0.0	0.0	3.290275147614081E-5	0.0
7	0.0	0.0	0.0	3.290275147614081E-5	0.0
8	0.0	0.0	0.0	3.290275147614081E-5	0.0
9	0.0	0.0	0.0	4.935412721421123E-5	0.0
10	0.0	0.0	0.0	4.935412721421123E-5	0.0
11	0.0	0.0	0.0	4.935412721421123E-5	0.0
12	0.0	0.0	0.0	6.580550295228163E-5	0.0
13	0.0	0.0	0.0	6.580550295228163E-5	0.0
14	0.0	0.0	0.0	1.3161100590456325E-4	0.0
15	0.0	0.0	0.0	1.6451375738070408E-4	0.0
16	0.0	0.0	0.0	2.632220118091265E-4	0.0
17	0.0	0.0	0.0	2.632220118091265E-4	0.0
18	0.0	0.0	0.0	3.783816419756194E-4	0.0
19	0.0	0.0	0.0	4.112843934517602E-4	0.0
20	0.0	0.0	0.0	6.251522780466755E-4	0.0
21	0.0	0.0	0.0	6.909577809989571E-4	0.0
22	0.0	0.0	0.0	8.390201626415908E-4	0.0
23	0.0	0.0	0.0	0.0011186935501887878	0.0
24	0.0	0.0	0.0	0.001529977943640548	0.0
25	0.0	0.0	0.0	0.0019248109613542379	0.0
26	0.0	0.0	0.0	0.0024348036092344204	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5565	0.0	23.60302	1
GTATCAA	19095	0.0	22.213509	1
AGTCCGT	1375	0.0	15.705414	6
GTCCTAG	1110	0.0	15.585462	1
TGCGCTA	890	0.0	15.46125	10
GTCTAGG	495	0.0	15.209381	1
CGGACGG	3980	0.0	15.194546	28
CCTAGAC	565	0.0	15.00694	3
ATCAACG	28565	0.0	14.757442	3
ACTGGTA	2925	0.0	14.713021	8
GCGCTAT	950	0.0	14.653177	11
AACGCAG	28740	0.0	14.621488	6
CGCTTCA	570	0.0	14.597035	14
TCAACGC	28925	0.0	14.555502	4
AACTGGT	2975	0.0	14.358191	7
CTGGTAC	2905	0.0	14.265475	9
TACGCCG	2395	0.0	14.095632	23
CGGCCGA	2850	0.0	14.090594	32
TCCTAGA	750	0.0	14.085624	2
GTCCTAC	615	0.0	14.064928	1
>>END_MODULE