FastQCFastQC Report
Thu 2 Feb 2017
SRR4062735_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062735_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6078519
Sequences flagged as poor quality0
Sequence length38
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCG202820.333666802719544No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT164190.2701151382433781No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACT152400.250718966248193No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGT135880.22354129352890073No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT104750.17232816085628752No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCA96470.15870642174516522No Hit
ACTTAGAACTGGTACGGACAAGGGGAATCTGACTGTCT96030.1579825612126901No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCC95490.15709418692283433No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACA91280.15016815773710668No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTA85660.14092248457231113No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGG82430.13560869020891436No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTAT82040.13496708655512962No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTT80570.13254873432163328No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCG76210.12537593449983458No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTT74900.12322080427814736No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAG73970.1216908263345068No Hit
ATTCCATTCCATTCCATTCCATTCCATTCCATTCCATT73150.12034181352398503No Hit
GTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGA72650.11951924473708152No Hit
CCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTTGAA67110.11040518257819051No Hit
GAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCG64820.10663781753417238No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGC63620.10466365244560394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54450.023.304791
GTATCAA173000.021.6533551
GTCTAGG5600.018.0020681
TCTAGGA6750.016.5934072
GACCCGC6500.015.5070532
TCCGTGC12650.015.4322228
CTAGGAC12050.015.4038783
CGGACGG37050.015.32999928
CGTGCCA13150.015.20912210
TACGCCG21750.015.15352223
AGTCCGT16350.014.6804656
TGCGCTA8000.014.59999610
CCTAGAC5200.014.4628443
CTAGACC1452.0327207E-814.3461224
AGGACGC6600.014.3047915
GGACCGT3700.014.2717936
TTAGAAC27300.014.1258163
GCCGGAC41800.014.12381726
AGGTCGC5250.014.0205436
ATCAACG276450.013.6716993
Produced by FastQC (version 0.11.5)