##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062735_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6078519
Sequences flagged as poor quality	0
Sequence length	38
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.237349591240893	32.0	32.0	32.0	32.0	32.0
2	31.34740699173598	32.0	32.0	32.0	32.0	32.0
3	31.422987079583038	32.0	32.0	32.0	32.0	32.0
4	31.488512909147772	32.0	32.0	32.0	32.0	32.0
5	31.420737518464612	32.0	32.0	32.0	32.0	32.0
6	34.92073398141883	36.0	36.0	36.0	36.0	36.0
7	34.964416990388614	36.0	36.0	36.0	36.0	36.0
8	34.89371374836535	36.0	36.0	36.0	36.0	36.0
9	35.00680165678515	36.0	36.0	36.0	36.0	36.0
10	34.89946136550696	36.0	36.0	36.0	32.0	36.0
11	35.040414285124385	36.0	36.0	36.0	36.0	36.0
12	34.98151539873446	36.0	36.0	36.0	36.0	36.0
13	35.00620792663476	36.0	36.0	36.0	36.0	36.0
14	34.9564370860731	36.0	36.0	36.0	36.0	36.0
15	34.934219832166356	36.0	36.0	36.0	36.0	36.0
16	34.94666019798573	36.0	36.0	36.0	36.0	36.0
17	34.923849049414834	36.0	36.0	36.0	36.0	36.0
18	34.92063790538452	36.0	36.0	36.0	32.0	36.0
19	34.88658618982683	36.0	36.0	36.0	32.0	36.0
20	34.87381350621755	36.0	36.0	36.0	32.0	36.0
21	34.86599647052185	36.0	36.0	36.0	32.0	36.0
22	34.866528014471946	36.0	36.0	36.0	32.0	36.0
23	34.82321680659384	36.0	36.0	36.0	32.0	36.0
24	34.75789234186814	36.0	36.0	36.0	32.0	36.0
25	34.74303658506291	36.0	36.0	36.0	32.0	36.0
26	34.6737053877762	36.0	36.0	36.0	32.0	36.0
27	34.68511013949286	36.0	36.0	36.0	32.0	36.0
28	34.6508027695562	36.0	36.0	36.0	32.0	36.0
29	34.5770104527106	36.0	36.0	36.0	32.0	36.0
30	34.57679592677098	36.0	36.0	36.0	32.0	36.0
31	34.52835353480017	36.0	36.0	36.0	32.0	36.0
32	34.56047007502979	36.0	36.0	36.0	32.0	36.0
33	34.515079248744634	36.0	36.0	36.0	32.0	36.0
34	34.46548361533459	36.0	36.0	36.0	32.0	36.0
35	34.44889207387523	36.0	36.0	36.0	32.0	36.0
36	34.39657900222077	36.0	36.0	36.0	32.0	36.0
37	34.43418964389188	36.0	36.0	36.0	32.0	36.0
38	34.07406623225164	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	11.0
20	53.0
21	157.0
22	489.0
23	1467.0
24	3817.0
25	9753.0
26	20644.0
27	40042.0
28	69446.0
29	109724.0
30	165221.0
31	237995.0
32	338756.0
33	537511.0
34	1322091.0
35	3221339.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.59963474526773	22.94666787321383	13.15310260860981	22.300594772908628
2	14.121698743015978	22.216023708410503	40.313889010446296	23.34838853812722
3	16.147123995170535	29.13969669256607	30.913533378772033	23.799645933491366
4	10.997881552164044	16.18813633018766	39.247515878487924	33.56646623916038
5	13.82248561984537	37.49507281053467	32.85566740303061	15.826774166589344
6	30.002671706496592	40.81156704140751	16.8314712548071	12.35428999728881
7	25.842363533586948	33.91550075248927	23.80476934081677	16.437366373107015
8	26.53849096181134	34.702350545992964	20.835091615769016	17.924066876426675
9	25.7395654845585	13.777089350332702	22.1214606470761	38.361884518032696
10	15.945330258652557	27.554021468914097	30.813759991629464	25.68688828080388
11	35.96190528210735	25.364835548840137	21.075519173547246	17.59773999550526
12	21.89558279180719	26.57191741383565	32.413654684543886	19.118845109813275
13	26.515303690827725	25.005095828724876	27.400735806410164	21.078864674037234
14	21.83400265755524	19.669659665454695	27.70645283826537	30.789884838724696
15	25.78955498864115	27.81866767217475	23.120977330168746	23.270800009015353
16	25.188808388625056	30.25821923340841	22.865182412091634	21.687789965874902
17	19.740894122400537	28.3042300270839	28.877692082561556	23.077183767954004
18	21.043431796462265	30.051793866236164	27.490841107842222	21.413933229459346
19	23.98539841694992	24.860529349336574	28.02815949082334	23.12591274289017
20	26.32637983035012	26.118417989645177	25.638070720844997	21.917131459159705
21	26.747570251990986	25.604461355749784	25.772841408035617	21.875126984223602
22	24.10923585473233	26.959207319811725	27.98587221192274	20.945684613533203
23	23.12782624818027	28.08305457802234	27.167056921360157	21.622062252437235
24	23.41440406783297	26.06840580740144	26.922001888946962	23.595188235818625
25	24.25485451947031	26.657367240102058	25.382252331204135	23.705525909223493
26	24.53288778613471	27.499433250012583	25.8061257694721	22.161553194380605
27	21.908894494426516	27.934068743457484	28.671897222231816	21.48513953988418
28	21.262267996530078	28.620573531151255	27.87683973678457	22.2403187355341
29	22.697996008567216	25.66223779180422	27.778032774101717	23.861733425526843
30	23.593641806499246	26.574466576480226	26.731626568906012	23.100265048114515
31	24.822855698896394	27.97359027750016	23.86099311361863	23.342560909984815
32	21.282832216202664	26.983612291086033	27.998053473222672	23.735502019488628
33	22.008124018366974	27.725585788248747	28.27274209392123	21.99354809946304
34	22.32626072238978	25.02622760577042	29.402622579611908	23.244889092227893
35	23.724611208750026	25.776706464189715	26.318976711267993	24.17970561579227
36	24.525464179679293	27.380945917911916	25.389934620587677	22.703655281821113
37	22.6145875335752	26.5442947533766	27.290019164207596	23.5510985488406
38	21.891872950568857	28.626658217235917	27.848672274614394	21.63279655758083
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19.0
1	116.0
2	213.0
3	213.0
4	814.0
5	1415.0
6	1415.0
7	2162.0
8	2909.0
9	3890.5
10	4872.0
11	4872.0
12	8126.0
13	11380.0
14	17341.5
15	23303.0
16	23303.0
17	35391.5
18	47480.0
19	47480.0
20	61786.0
21	76092.0
22	89849.0
23	103606.0
24	103606.0
25	122285.5
26	140965.0
27	140965.0
28	157778.5
29	174592.0
30	198610.0
31	222628.0
32	222628.0
33	259064.0
34	295500.0
35	295500.0
36	371956.5
37	448413.0
38	496648.0
39	544883.0
40	544883.0
41	543933.5
42	542984.0
43	542484.0
44	541984.0
45	541984.0
46	543395.5
47	544807.0
48	544807.0
49	498654.5
50	452502.0
51	425266.5
52	398031.0
53	398031.0
54	362318.0
55	326605.0
56	326605.0
57	287939.0
58	249273.0
59	243359.0
60	237445.0
61	237445.0
62	210148.5
63	182852.0
64	164342.5
65	145833.0
66	145833.0
67	133052.5
68	120272.0
69	120272.0
70	104969.5
71	89667.0
72	79659.0
73	69651.0
74	69651.0
75	54111.0
76	38571.0
77	38571.0
78	28989.0
79	19407.0
80	15747.5
81	12088.0
82	12088.0
83	8375.0
84	4662.0
85	4662.0
86	3351.0
87	2040.0
88	1419.0
89	798.0
90	798.0
91	549.0
92	300.0
93	237.0
94	174.0
95	174.0
96	197.5
97	221.0
98	221.0
99	151.5
100	82.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009360832794962063
2	1.6451375738070407E-5
3	0.0
4	1.974165088568449E-4
5	4.935412721421123E-5
6	1.1515963016649287E-4
7	4.44187144927901E-4
8	0.0023689981062821387
9	0.004178649437469884
10	9.212770413319429E-4
11	0.004951864097159192
12	3.125761390233378E-4
13	2.9612476328526733E-4
14	0.0
15	0.0
16	3.290275147614081E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.3161100590456325E-4
22	3.290275147614081E-5
23	1.6451375738070408E-4
24	0.0
25	1.3161100590456325E-4
26	1.6451375738070407E-5
27	9.870825442842245E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	4.935412721421123E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	6078519.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.67490705045078
#Duplication Level	Percentage of deduplicated	Percentage of total
1	55.686414838977086	12.626842804466484
2	14.973163002399922	6.790301586613334
3	7.821789502778224	5.320750498310637
4	4.852904457979929	4.401566300376524
5	3.37629424702837	3.8278579113169995
6	2.3900438972410822	3.251641392986321
7	1.837568640720376	2.916668868901039
8	1.4016110535536188	2.5425120288170158
9	1.079909500943336	2.203816280110995
>10	5.963982315850656	23.483089280967288
>50	0.31281096570208816	4.934368163812711
>100	0.2534124683354571	11.653541157322671
>500	0.03039152261521267	4.743124561243054
>1k	0.01781320494199774	7.4906694121259445
>5k	0.0015268461378847309	2.560229920295982
>10k+	3.6353479473445966E-4	1.2530198323331454
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCG	20282	0.333666802719544	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	16419	0.2701151382433781	No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACT	15240	0.250718966248193	No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGT	13588	0.22354129352890073	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	10475	0.17232816085628752	No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCA	9647	0.15870642174516522	No Hit
ACTTAGAACTGGTACGGACAAGGGGAATCTGACTGTCT	9603	0.1579825612126901	No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCC	9549	0.15709418692283433	No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACA	9128	0.15016815773710668	No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTA	8566	0.14092248457231113	No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGG	8243	0.13560869020891436	No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTAT	8204	0.13496708655512962	No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTT	8057	0.13254873432163328	No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCG	7621	0.12537593449983458	No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTT	7490	0.12322080427814736	No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAG	7397	0.1216908263345068	No Hit
ATTCCATTCCATTCCATTCCATTCCATTCCATTCCATT	7315	0.12034181352398503	No Hit
GTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGA	7265	0.11951924473708152	No Hit
CCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTTGAA	6711	0.11040518257819051	No Hit
GAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCG	6482	0.10663781753417238	No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGC	6362	0.10466365244560394	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.290275147614081E-5	0.0
2	0.0	0.0	0.0	3.290275147614081E-5	0.0
3	0.0	0.0	0.0	3.290275147614081E-5	0.0
4	0.0	0.0	0.0	3.290275147614081E-5	0.0
5	0.0	0.0	0.0	3.290275147614081E-5	0.0
6	0.0	0.0	0.0	3.290275147614081E-5	0.0
7	0.0	0.0	0.0	3.290275147614081E-5	0.0
8	0.0	0.0	0.0	3.290275147614081E-5	0.0
9	0.0	0.0	0.0	4.935412721421123E-5	0.0
10	0.0	0.0	0.0	4.935412721421123E-5	0.0
11	0.0	0.0	0.0	4.935412721421123E-5	0.0
12	0.0	0.0	0.0	6.580550295228163E-5	0.0
13	0.0	0.0	0.0	6.580550295228163E-5	0.0
14	0.0	0.0	0.0	1.4806238164263367E-4	0.0
15	0.0	0.0	0.0	1.809651331187745E-4	0.0
16	0.0	0.0	0.0	3.125761390233378E-4	0.0
17	0.0	0.0	0.0	3.125761390233378E-4	0.0
18	0.0	0.0	0.0	4.2773576918983064E-4	0.0
19	0.0	0.0	0.0	4.606385206659714E-4	0.0
20	0.0	0.0	0.0	7.074091567370275E-4	0.0
21	0.0	0.0	0.0	7.896660354273796E-4	0.0
22	0.0	0.0	0.0	9.212770413319428E-4	0.0
23	0.0	0.0	0.0	0.0011844990531410693	0.0
24	0.0	0.0	0.0	0.0015793320708547593	0.0
25	0.0	0.0	0.0	0.001891908209878097	0.0
26	0.0	0.0	0.0	0.002336095354805998	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5445	0.0	23.30479	1
GTATCAA	17300	0.0	21.653355	1
GTCTAGG	560	0.0	18.002068	1
TCTAGGA	675	0.0	16.593407	2
GACCCGC	650	0.0	15.50705	32
TCCGTGC	1265	0.0	15.432222	8
CTAGGAC	1205	0.0	15.403878	3
CGGACGG	3705	0.0	15.329999	28
CGTGCCA	1315	0.0	15.209122	10
TACGCCG	2175	0.0	15.153522	23
AGTCCGT	1635	0.0	14.680465	6
TGCGCTA	800	0.0	14.599996	10
CCTAGAC	520	0.0	14.462844	3
CTAGACC	145	2.0327207E-8	14.346122	4
AGGACGC	660	0.0	14.304791	5
GGACCGT	370	0.0	14.271793	6
TTAGAAC	2730	0.0	14.125816	3
GCCGGAC	4180	0.0	14.123817	26
AGGTCGC	525	0.0	14.020543	6
ATCAACG	27645	0.0	13.671699	3
>>END_MODULE