FastQCFastQC Report
Thu 2 Feb 2017
SRR4062734_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062734_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15021
Sequences flagged as poor quality0
Sequence length38
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT6734.480394114905799No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6604.393848611943279No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT4432.949204447107383No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT2671.7775114839225084No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC2431.6177351707609346No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA2351.564476399707077No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2131.4180147793089675No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA1871.2449237733839291No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG1090.7256507556088143No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA1050.6990213700818854No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA960.6391052526462951No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA940.6257905598828306No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT910.605818520737634No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT880.5858464815924372No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG870.579189135210705No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT840.5592170960655083No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA830.5525597496837761No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.5459024033020438No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG610.4060981292856668No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.4060981292856668No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA610.4060981292856668No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA590.39278343652220227No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC580.38612609014047No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG490.32620997270487984No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC470.3128952799414153No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA470.3128952799414153No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA460.3062379335596831No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC410.2729512016510219No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA410.2729512016510219No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA390.2596365088875574No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA380.2529791625058252No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA380.2529791625058252No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC360.2396644697423607No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT360.2396644697423607No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG350.23300712336062845No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT330.21969243059716398No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT330.21969243059716398No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT330.21969243059716398No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA330.21969243059716398No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC300.19972039145196727No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA290.193063045070235No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAA260.1730910059250383No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT260.1730910059250383No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA250.16643365954330605No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG240.15977631316157379No Hit
GGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA240.15977631316157379No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA240.15977631316157379No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT230.15311896677984155No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC230.15311896677984155No Hit
GGTATCAACGCAGAGTACATGGGCAGTGGTATCAACGC220.1464616203981093No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA220.1464616203981093No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT210.13980427401637707No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTT210.13980427401637707No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCA200.13314692763464483No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA190.1264895812529126No Hit
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCA190.1264895812529126No Hit
GAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA180.11983223487118035No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTT180.11983223487118035No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT180.11983223487118035No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG180.11983223487118035No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA180.11983223487118035No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTA170.1131748884894481No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.1131748884894481No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT160.10651754210771586No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT160.10651754210771586No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG160.10651754210771586No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG160.10651754210771586No Hit
ACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAG160.10651754210771586No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT160.10651754210771586No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT160.10651754210771586No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGAGT450.0085940117.729636
ATGGGAG600.002336469715.9566665
ATGGAAG951.2873872E-515.1168415
TGGAAGC951.2873872E-515.1168416
CATGGAA1002.0919311E-514.3614
ATGGGGT1003.454685E-412.7653335
AGCAGTG1353.3638993E-410.67335510
ACATGGA1404.7088612E-410.29216532
GAAGCAG1404.7088612E-410.2921658
AAGCAGT1404.7088612E-410.2921659
TACATGG5400.010.046792
TGCTTCC1300.00302527989.85232726
CTGCTTC1300.00302527989.85232725
CATGGGG1804.2827145E-59.7512964
GTACATG5600.09.6879771
GGAAGCA1508.8882184E-49.606027
ACATGGG4050.09.4558033
AACGCAG5500.09.2838786
CATGGGA1400.00574074369.1180954
ACTGCTT1450.00745182538.83312124
Produced by FastQC (version 0.11.5)