##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062734_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15021
Sequences flagged as poor quality	0
Sequence length	38
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.08208508088676	32.0	32.0	32.0	14.0	32.0
2	29.05945010318887	32.0	32.0	32.0	14.0	32.0
3	29.581186339125225	32.0	32.0	32.0	21.0	32.0
4	29.39577924239398	32.0	32.0	32.0	21.0	32.0
5	29.68570667731842	32.0	32.0	32.0	21.0	32.0
6	32.973770055255976	36.0	36.0	36.0	21.0	36.0
7	32.48485453698156	36.0	32.0	36.0	21.0	36.0
8	32.337594035017645	36.0	32.0	36.0	14.0	36.0
9	32.11264230077891	36.0	32.0	36.0	14.0	36.0
10	32.284268690499964	36.0	32.0	36.0	14.0	36.0
11	32.88709140536582	36.0	36.0	36.0	21.0	36.0
12	32.697290460022636	36.0	32.0	36.0	21.0	36.0
13	32.695492976499565	36.0	32.0	36.0	21.0	36.0
14	32.61939950735637	36.0	32.0	36.0	21.0	36.0
15	32.80274282670927	36.0	36.0	36.0	21.0	36.0
16	32.59856201318154	36.0	32.0	36.0	21.0	36.0
17	32.45136808468145	36.0	32.0	36.0	14.0	36.0
18	32.570601158378274	36.0	32.0	36.0	21.0	36.0
19	32.508354969709075	36.0	32.0	36.0	14.0	36.0
20	32.092403967778445	36.0	32.0	36.0	14.0	36.0
21	32.040676386392384	36.0	32.0	36.0	14.0	36.0
22	32.0860794887158	36.0	32.0	36.0	14.0	36.0
23	32.209040676386394	36.0	32.0	36.0	14.0	36.0
24	32.33466480260968	36.0	32.0	36.0	14.0	36.0
25	32.244324612209574	36.0	32.0	36.0	14.0	36.0
26	32.287863657546104	36.0	32.0	36.0	14.0	36.0
27	32.18667199254377	36.0	32.0	36.0	14.0	36.0
28	32.25411091139072	36.0	32.0	36.0	14.0	36.0
29	32.05891751547833	36.0	32.0	36.0	14.0	36.0
30	31.912256174688768	36.0	32.0	36.0	14.0	36.0
31	32.02310099194461	36.0	32.0	36.0	14.0	36.0
32	31.852406630716995	36.0	32.0	36.0	14.0	36.0
33	31.53525064909127	36.0	32.0	36.0	14.0	36.0
34	31.42167631981892	36.0	32.0	36.0	14.0	36.0
35	31.123693495772585	36.0	32.0	36.0	14.0	36.0
36	31.053791358764396	36.0	32.0	36.0	14.0	36.0
37	31.049863524399175	36.0	32.0	36.0	14.0	36.0
38	29.97916250582518	36.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	1.0
13	0.0
14	24.0
15	42.0
16	61.0
17	45.0
18	72.0
19	76.0
20	107.0
21	151.0
22	255.0
23	354.0
24	301.0
25	337.0
26	348.0
27	441.0
28	534.0
29	695.0
30	856.0
31	1038.0
32	1199.0
33	1546.0
34	2433.0
35	4104.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.96908109548877	19.324315319517556	15.419470913573665	13.287132671420004
2	12.127872127872127	27.998667998667997	38.108558108558114	21.764901764901765
3	18.704221600745772	39.97869223598349	26.528166200559326	14.788919962711415
4	9.546634711404034	23.300712336062844	30.67705212702217	36.47560082551095
5	8.887557419612541	45.17675254643499	27.53478463484455	18.400905399107916
6	24.452433260102524	36.954929764995676	20.07855668730444	18.514080287597366
7	20.99061314160176	40.75627454896478	20.717661939950734	17.535450369482724
8	31.84208774382531	28.313694161507225	19.492710205712	20.35150788895546
9	30.4773317355702	18.187870314892486	19.639171826110115	31.6956261234272
10	18.580653751414687	32.32807402969177	26.05019639171826	23.041075827175288
11	27.902796271637815	30.81225033288948	18.375499334221036	22.90945406125166
12	25.947133630734402	33.970304281243756	26.31333644050869	13.76922564751315
13	32.44755244755245	27.865467865467863	25.66100566100566	14.025974025974024
14	26.796297529466607	24.239195578344543	30.25238063528002	18.71212625690884
15	24.197415745304383	27.221260157186627	35.50686026375383	13.074463833755162
16	16.867550109875474	31.990410867683295	31.197975627622025	19.944063394819207
17	15.790875790875791	30.46953046953047	31.22877122877123	22.51082251082251
18	18.40458116926355	26.35504061792516	38.24077773338661	16.99960047942469
19	17.7054201624717	31.48888001065388	38.05433479824211	12.751365028632309
20	22.321369115002994	21.755343943530665	41.479656389425315	14.44363055204102
21	24.678697476193648	26.3235000332956	32.47652660318306	16.521275887327697
22	21.45045285029302	27.637187000532766	33.71070857751731	17.2016515716569
23	18.6134789557805	29.37533297815663	34.40996270644646	17.60122535961641
24	16.016249334043685	33.71070857751731	35.00266382525306	15.270378263185936
25	20.293040293040292	28.498168498168496	33.59307359307359	17.615717615717617
26	15.758625283069136	29.339283335553485	36.34607699480485	18.556014386572535
27	18.13399041022909	31.779435269046353	36.80740543420352	13.279168886521045
28	16.37925294626806	28.870097876023703	36.8732938278181	17.877355349890138
29	14.031699520511454	32.49866808737347	39.697655833777304	13.771976558337773
30	17.198215593581466	31.207137625674147	37.525800652506824	14.068846128237567
31	13.791036824931743	33.50869015116202	34.50755810081907	18.192714923087166
32	12.64482620854974	34.471966972965774	36.41630043947263	16.466906379011853
33	13.624558833322235	34.048078843976825	35.47313045215423	16.854231870546712
34	13.16595631326585	34.78289824187533	36.80074587107086	15.25039957378796
35	15.404595404595407	34.538794538794534	35.35131535131535	14.705294705294705
36	14.571124134256793	37.71976558337773	33.597496004262126	14.111614278103357
37	15.018315018315018	38.24175824175824	34.26573426573427	12.474192474192474
38	13.716873085630576	39.20628579038487	32.02823278732188	15.048608336662673
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	783.0
1	444.5
2	106.0
3	106.0
4	171.0
5	236.0
6	236.0
7	271.5
8	307.0
9	276.5
10	246.0
11	246.0
12	284.0
13	322.0
14	365.5
15	409.0
16	409.0
17	588.5
18	768.0
19	768.0
20	824.0
21	880.0
22	677.0
23	474.0
24	474.0
25	353.0
26	232.0
27	232.0
28	290.0
29	348.0
30	433.0
31	518.0
32	518.0
33	407.5
34	297.0
35	297.0
36	293.5
37	290.0
38	394.5
39	499.0
40	499.0
41	631.0
42	763.0
43	925.5
44	1088.0
45	1088.0
46	1662.5
47	2237.0
48	2237.0
49	1849.5
50	1462.0
51	1047.5
52	633.0
53	633.0
54	484.0
55	335.0
56	335.0
57	361.0
58	387.0
59	364.5
60	342.0
61	342.0
62	314.5
63	287.0
64	281.5
65	276.0
66	276.0
67	227.0
68	178.0
69	178.0
70	151.0
71	124.0
72	100.5
73	77.0
74	77.0
75	70.5
76	64.0
77	64.0
78	43.0
79	22.0
80	18.0
81	14.0
82	14.0
83	9.5
84	5.0
85	5.0
86	2.5
87	0.0
88	1.0
89	2.0
90	2.0
91	1.5
92	1.0
93	2.0
94	3.0
95	3.0
96	1.5
97	0.0
98	0.0
99	3.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09320284934425138
2	0.039944078290393446
3	0.019972039145196723
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.006657346381732241
12	0.013314692763464482
13	0.039944078290393446
14	0.026629385526928964
15	0.04660142467212569
16	0.026629385526928964
17	0.039944078290393446
18	0.019972039145196723
19	0.019972039145196723
20	0.026629385526928964
21	0.026629385526928964
22	0.03328673190866121
23	0.03328673190866121
24	0.03328673190866121
25	0.039944078290393446
26	0.04660142467212569
27	0.03328673190866121
28	0.013314692763464482
29	0.03328673190866121
30	0.013314692763464482
31	0.026629385526928964
32	0.019972039145196723
33	0.026629385526928964
34	0.03328673190866121
35	0.039944078290393446
36	0.03328673190866121
37	0.039944078290393446
38	0.019972039145196723
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	15021.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.70900738965448
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.77734016016805	44.51101790826177
2	6.8924773532886965	6.990213700818853
3	1.509780753577524	2.296784501697623
4	0.8270972823946435	1.6776512881965249
5	0.47262701851122485	1.1983223487118035
6	0.31508467900748327	0.9586578789694428
7	0.23631350925561243	0.8388256440982624
8	0.24944203754759092	1.0119166500233008
9	0.3019561507155048	1.378070701018574
>10	1.1159249048181699	11.577125357832369
>50	0.1706708677957201	6.690633113640902
>100	0.10502822633582776	11.9965381798815
>500	0.02625705658395694	8.874242726849078
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	673	4.480394114905799	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	660	4.393848611943279	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	443	2.949204447107383	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	267	1.7775114839225084	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	243	1.6177351707609346	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	235	1.564476399707077	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	213	1.4180147793089675	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	187	1.2449237733839291	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	109	0.7256507556088143	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	105	0.6990213700818854	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	96	0.6391052526462951	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	94	0.6257905598828306	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	91	0.605818520737634	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	88	0.5858464815924372	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	87	0.579189135210705	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	84	0.5592170960655083	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	83	0.5525597496837761	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	82	0.5459024033020438	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	61	0.4060981292856668	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	61	0.4060981292856668	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	61	0.4060981292856668	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	59	0.39278343652220227	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	58	0.38612609014047	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	49	0.32620997270487984	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	47	0.3128952799414153	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	47	0.3128952799414153	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	46	0.3062379335596831	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	41	0.2729512016510219	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	41	0.2729512016510219	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA	39	0.2596365088875574	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA	38	0.2529791625058252	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA	38	0.2529791625058252	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC	36	0.2396644697423607	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	36	0.2396644697423607	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG	35	0.23300712336062845	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	33	0.21969243059716398	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	33	0.21969243059716398	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	33	0.21969243059716398	No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA	33	0.21969243059716398	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	30	0.19972039145196727	No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA	29	0.193063045070235	No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAA	26	0.1730910059250383	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT	26	0.1730910059250383	No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA	25	0.16643365954330605	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	24	0.15977631316157379	No Hit
GGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA	24	0.15977631316157379	No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	24	0.15977631316157379	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	23	0.15311896677984155	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	23	0.15311896677984155	No Hit
GGTATCAACGCAGAGTACATGGGCAGTGGTATCAACGC	22	0.1464616203981093	No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA	22	0.1464616203981093	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT	21	0.13980427401637707	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTT	21	0.13980427401637707	No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCA	20	0.13314692763464483	No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA	19	0.1264895812529126	No Hit
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCA	19	0.1264895812529126	No Hit
GAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA	18	0.11983223487118035	No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTT	18	0.11983223487118035	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT	18	0.11983223487118035	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	18	0.11983223487118035	No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	18	0.11983223487118035	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTA	17	0.1131748884894481	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	17	0.1131748884894481	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT	16	0.10651754210771586	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT	16	0.10651754210771586	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	16	0.10651754210771586	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	16	0.10651754210771586	No Hit
ACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAG	16	0.10651754210771586	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT	16	0.10651754210771586	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT	16	0.10651754210771586	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.006657346381732242	0.0
24	0.0	0.0	0.0	0.026629385526928968	0.0
25	0.0	0.0	0.0	0.05991611743559017	0.0
26	0.0	0.0	0.0	0.07988815658078689	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGGAGT	45	0.00859401	17.72963	6
ATGGGAG	60	0.0023364697	15.956666	5
ATGGAAG	95	1.2873872E-5	15.116841	5
TGGAAGC	95	1.2873872E-5	15.116841	6
CATGGAA	100	2.0919311E-5	14.361	4
ATGGGGT	100	3.454685E-4	12.765333	5
AGCAGTG	135	3.3638993E-4	10.673355	10
ACATGGA	140	4.7088612E-4	10.292165	32
GAAGCAG	140	4.7088612E-4	10.292165	8
AAGCAGT	140	4.7088612E-4	10.292165	9
TACATGG	540	0.0	10.04679	2
TGCTTCC	130	0.0030252798	9.852327	26
CTGCTTC	130	0.0030252798	9.852327	25
CATGGGG	180	4.2827145E-5	9.751296	4
GTACATG	560	0.0	9.687977	1
GGAAGCA	150	8.8882184E-4	9.60602	7
ACATGGG	405	0.0	9.455803	3
AACGCAG	550	0.0	9.283878	6
CATGGGA	140	0.0057407436	9.118095	4
ACTGCTT	145	0.0074518253	8.833121	24
>>END_MODULE