FastQCFastQC Report
Thu 2 Feb 2017
SRR4062734_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062734_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15021
Sequences flagged as poor quality0
Sequence length38
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT8185.445709340256974No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT6084.047666600093203No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3642.423274082950536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT3522.343385926369749No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA2961.9705745289927437No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2841.8906863724119565No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC2541.6909659809599895No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2141.4246721256906996No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA1480.9852872644963718No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1450.965315225351175No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1180.7855668730444045No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG1150.7655948338992078No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1110.7389654483722788No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT1000.6657346381732242No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG980.6524199454097597No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA920.6124758671193662No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA910.605818520737634No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA810.5392450569203115No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG800.5325877105385793No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA790.5259303641568471No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT770.5126156713933826No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA750.49930097862991807No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC670.4460422075760602No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT570.37946874375873774No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG570.37946874375873774No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA570.37946874375873774No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC560.3728113973770055No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC510.3395246654683443No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT480.31955262632314757No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA470.3128952799414153No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT460.3062379335596831No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG460.3062379335596831No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA450.2995805871779509No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA450.2995805871779509No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT400.26629385526928967No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC350.23300712336062845No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG350.23300712336062845No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT340.2263497769788962No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA340.2263497769788962No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA330.21969243059716398No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT310.20637773783369948No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC310.20637773783369948No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG310.20637773783369948No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA300.19972039145196727No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA300.19972039145196727No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTG290.193063045070235No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTT290.193063045070235No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA280.18640569868850276No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT280.18640569868850276No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT270.17974835230677053No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA270.17974835230677053No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT270.17974835230677053No Hit
GAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA260.1730910059250383No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT260.1730910059250383No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTT260.1730910059250383No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA260.1730910059250383No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG260.1730910059250383No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCA260.1730910059250383No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA260.1730910059250383No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG250.16643365954330605No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG240.15977631316157379No Hit
GTATCAACGCAGAGTACATGGGACGCAGAGTACTTTTT240.15977631316157379No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA230.15311896677984155No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA230.15311896677984155No Hit
ACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAG230.15311896677984155No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT210.13980427401637707No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT210.13980427401637707No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAA210.13980427401637707No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA200.13314692763464483No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA190.1264895812529126No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG190.1264895812529126No Hit
GGTATCAACGCAGAGTACATGGGTGGTATCAACGCAAA190.1264895812529126No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT190.1264895812529126No Hit
GGTATCAACGCAGAGTACATGGGCAGTGGTATCAACGC180.11983223487118035No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.11983223487118035No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTA180.11983223487118035No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT170.1131748884894481No Hit
TGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG170.1131748884894481No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG170.1131748884894481No Hit
CATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA160.10651754210771586No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA160.10651754210771586No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAA1050.00722199710.66666719
GGTATCA4800.08.6666671
ACATGGG3401.7877574E-78.03
TACATGG4701.1441443E-97.4893622
AACGCAG5903.45608E-117.05084755
GTACATG4902.1540473E-86.85714341
GTATCAA6107.6397555E-116.8196721
ACGCAGA7052.2755557E-95.9007096
CGCAGAG7052.2755557E-95.9007097
AGAGTAC7358.949428E-105.87755110
ATCAACG7358.949428E-105.8775512
TATCAAC7401.0513759E-95.83783771
TCAACGC7451.2350938E-95.7986583
CAACGCA7451.2350938E-95.7986585
AGTACTT4152.1703156E-45.783132612
GAGTACT4202.5565975E-45.71428611
CAGAGTA7305.0749804E-95.69863039
GCAGAGT7406.9339876E-95.62162168
CTTTTTT4752.2265656E-45.38947416
GAGTACA3900.0041398465.333333511
Produced by FastQC (version 0.11.5)