FastQCFastQC Report
Thu 2 Feb 2017
SRR4062733_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062733_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170925
Sequences flagged as poor quality0
Sequence length38
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT66463.8882550826385844No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT49162.8761152552289015No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA44662.6128418897177124No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC37892.2167617376042124No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT26161.530495831505046No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA24851.453854029545122No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23001.3456194237238555No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA14220.8319438350153576No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG14120.8260933157817757No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG13610.7962556676905075No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12620.7383355272780459No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA11470.6710545560918532No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA10810.632441129150212No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA10490.6137194676027498No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9640.5639900541173029No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA8770.5130905367851397No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG8610.5037297060114085No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8610.5037297060114085No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT8020.46921164253327485No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC7660.4481497732923797No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7540.4411291502120813No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC7510.4393739944420067No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG6680.3908146848032763No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA6560.3837940617229779No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC6100.35688167324850084No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT5080.2972063770659646No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC4980.29135585783238266No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG4950.28960070206230804No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA4790.28023987128857686No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4710.27555945590171127No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA4440.25976305397103994No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA4360.25508263858417435No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA4150.24279654819365218No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA3970.2322656135732046No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3720.2176393154892497No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT3650.21354395202574228No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA3450.2018429135585783No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA3380.19774755009507095No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA3350.19599239432499635No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC3150.18429135585783238No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCA2970.1737604212373848No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC2890.16908000585051924No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG2830.16556969431037005No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA2820.16498464238701185No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT2770.16205938277022086No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2710.15854907123007167No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT2710.15854907123007167No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2680.15679391545999707No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT2680.15679391545999707No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTG2650.15503875968992248No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA2630.15386865584320608No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2620.15328360391984788No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2540.1486031885329823No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT2500.1462629808395495No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA2370.13865730583589295No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT2290.13397689044902736No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA2220.12988152698551997No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT2200.12871142313880357No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTT2190.12812637121544537No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA2120.124031007751938No Hit
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCA2080.1216908000585052No Hit
GTATCAACGCAGAGTACATGGGACGCAGAGTACTTTTT2080.1216908000585052No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTA2060.12052069621178879No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA2060.12052069621178879No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA2010.11759543659499781No Hit
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA1980.1158402808249232No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAA1950.11408512505484862No Hit
GGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA1930.11291502120813221No Hit
GAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA1920.11232996928477403No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT1900.11115986543805763No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTA1880.10998976159134123No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG1830.10706450197455024No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAATC1810.10589439812783384No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT1770.10355419043440106No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACC200.00374683431.9961974
GCTCACC200.00374683431.9961976
TACCAAT350.002060818522.85442520
CTTCAAT502.7822083E-522.39733928
TCCATGA555.8014703E-520.36121630
ATGCTTC400.00447122419.99762325
GATCAAC400.00447122419.99762321
CAATGTA400.00447122419.99762331
GGATCAA450.00882638717.77566720
AATGCTT450.00882638717.77566724
TGGGATC450.00882638717.77566718
ATACCAA450.00882638717.77566719
CCATGAA652.0891515E-417.2287231
TGCTTCA703.676775E-415.99809826
TACATGC600.002432436415.99809831
AATGTAA600.002432436415.99809832
CATGAAC756.211505E-414.93155932
ACATGCA756.211505E-414.93155932
AAAAAGT1401.2405508E-814.8684281
GCTTCAA650.004145614314.76747527
Produced by FastQC (version 0.11.5)