##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062733_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	170925
Sequences flagged as poor quality	0
Sequence length	38
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.708913266052363	32.0	32.0	32.0	14.0	32.0
2	29.822325581395347	32.0	32.0	32.0	21.0	32.0
3	30.163580517770953	32.0	32.0	32.0	21.0	32.0
4	29.881673248500803	32.0	32.0	32.0	21.0	32.0
5	30.302594705280093	32.0	32.0	32.0	21.0	32.0
6	33.61549802544976	36.0	36.0	36.0	21.0	36.0
7	33.071001901418754	36.0	36.0	36.0	21.0	36.0
8	32.88619569986837	36.0	36.0	36.0	14.0	36.0
9	32.63325435132368	36.0	36.0	36.0	14.0	36.0
10	32.87468772853591	36.0	36.0	36.0	14.0	36.0
11	33.39273950563113	36.0	36.0	36.0	21.0	36.0
12	33.21753985666228	36.0	36.0	36.0	21.0	36.0
13	33.203223636097704	36.0	36.0	36.0	21.0	36.0
14	33.152347520842476	36.0	36.0	36.0	21.0	36.0
15	33.32948369167764	36.0	36.0	36.0	21.0	36.0
16	33.23810443176832	36.0	36.0	36.0	21.0	36.0
17	33.11512066695919	36.0	36.0	36.0	21.0	36.0
18	33.175106040661106	36.0	36.0	36.0	21.0	36.0
19	33.07545414655551	36.0	36.0	36.0	21.0	36.0
20	32.75194968553459	36.0	36.0	36.0	14.0	36.0
21	32.563065672078395	36.0	36.0	36.0	14.0	36.0
22	32.66239578762615	36.0	36.0	36.0	14.0	36.0
23	32.720936083077376	36.0	36.0	36.0	14.0	36.0
24	32.76499049290624	36.0	36.0	36.0	14.0	36.0
25	32.74034225537516	36.0	36.0	36.0	14.0	36.0
26	32.68372093023256	36.0	36.0	36.0	14.0	36.0
27	32.67967237092292	36.0	36.0	36.0	14.0	36.0
28	32.65288869387158	36.0	36.0	36.0	14.0	36.0
29	32.602229047828	36.0	36.0	36.0	14.0	36.0
30	32.45115694017844	36.0	36.0	36.0	14.0	36.0
31	32.40458680707913	36.0	32.0	36.0	14.0	36.0
32	32.27270440251572	36.0	32.0	36.0	14.0	36.0
33	32.017030861488955	36.0	32.0	36.0	14.0	36.0
34	31.79379259909317	36.0	32.0	36.0	14.0	36.0
35	31.506046511627908	36.0	32.0	36.0	14.0	36.0
36	31.4219511481644	36.0	32.0	36.0	14.0	36.0
37	31.30254205060699	36.0	32.0	36.0	14.0	36.0
38	30.249969284774025	36.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	0.0
9	1.0
10	1.0
11	1.0
12	0.0
13	1.0
14	206.0
15	399.0
16	366.0
17	486.0
18	604.0
19	785.0
20	1029.0
21	1163.0
22	1613.0
23	2015.0
24	2330.0
25	2818.0
26	3570.0
27	4530.0
28	5964.0
29	7789.0
30	9875.0
31	12024.0
32	14754.0
33	18771.0
34	30209.0
35	49619.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	54.084985503851	16.10390371042844	15.363575131051043	14.447535654669515
2	11.452705273571267	25.185414995931698	41.1858364601687	22.176043270328332
3	18.87002211742127	39.665664166266836	25.598282096591106	15.866031619720783
4	10.054411420547625	19.241750526562136	31.074186754036976	39.62965129885326
5	9.31700638880438	44.475907420842006	28.065104958929115	18.14198123142449
6	24.9741114523914	34.6690068743601	22.850958022524498	17.505923650724
7	23.919262834576568	36.23343571741992	22.079859587538394	17.767441860465116
8	34.268446795066815	25.310664388851183	20.58107696988135	19.83981184620065
9	32.02143655330178	15.113823183538786	19.177641394078034	33.6870988690814
10	18.474400753555695	30.515495281501025	27.690246485259447	23.319857479683833
11	30.635205738323553	27.40915393661399	19.3984355345452	22.557204790517257
12	26.628676406058936	29.726598838059687	28.260424406597203	15.384300349284171
13	34.09282404156719	24.746056265505782	26.25275008191733	14.90836961100969
14	28.378259775670095	20.16745753588167	30.997197338934757	20.457085349513484
15	25.358574479913393	23.94826930391784	37.09044093981333	13.602715276355443
16	18.54517369095473	28.653595230154412	32.41587530060208	20.385355778288776
17	17.426897847165737	26.3167750203346	33.16851092216676	23.0878162103329
18	20.149546270997035	23.486253561670285	39.49343833556639	16.870761831766295
19	18.512450851900393	30.358313049990638	37.43914997191537	13.690086126193595
20	23.52631424977328	18.878974929058305	42.063598864932864	15.531111956235556
21	25.487809872628237	23.769431946500347	31.840600993464662	18.902157187406754
22	21.66482362371499	25.663934282354177	33.22509112823484	19.446150965695995
23	18.0923516195469	27.98340666541846	33.841977157835615	20.082264557199025
24	16.66422857945336	31.73921438978578	34.59353185214659	17.00302517861427
25	21.821586354194096	25.64089060534246	32.85818777611984	19.679335264343603
26	16.763804039978467	26.609203473538845	36.40135290124757	20.22563958523512
27	19.867297801754148	30.071558548701354	35.790391377867756	14.27075227167674
28	17.75794985811661	26.73375654565135	36.641605476406404	18.866688119825646
29	14.928848944436643	30.84831250292562	39.53037962832936	14.692458924308383
30	19.16697382970881	28.42106494889392	37.42240476483013	14.989556456567147
31	14.673118103418014	30.981523303572473	35.26193847342	19.083420119589512
32	13.641948582327954	33.10436827878348	35.263348818704145	17.99033432018442
33	14.19109472821953	32.62301796267041	34.07699959042771	19.108887718682347
34	13.56625434459515	33.69299364548103	36.06335794782975	16.677394062094066
35	17.12122364671531	32.715814603947365	34.73513595749536	15.427825791841965
36	17.049251312178537	35.44491190703281	33.24185630109012	14.263980479698535
37	17.169169918846652	36.42363569343108	33.24303292356841	13.164161464153858
38	15.110666456040581	37.839561429683066	30.35472943324031	16.695042681036046
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1441.0
1	1033.5
2	626.0
3	626.0
4	1522.0
5	2418.0
6	2418.0
7	2663.5
8	2909.0
9	2751.5
10	2594.0
11	2594.0
12	3202.0
13	3810.0
14	4221.5
15	4633.0
16	4633.0
17	6441.0
18	8249.0
19	8249.0
20	8584.5
21	8920.0
22	6868.0
23	4816.0
24	4816.0
25	3784.0
26	2752.0
27	2752.0
28	3791.5
29	4831.0
30	5997.5
31	7164.0
32	7164.0
33	5692.5
34	4221.0
35	4221.0
36	4177.5
37	4134.0
38	4918.0
39	5702.0
40	5702.0
41	7460.0
42	9218.0
43	11612.0
44	14006.0
45	14006.0
46	22007.0
47	30008.0
48	30008.0
49	24219.0
50	18430.0
51	12904.0
52	7378.0
53	7378.0
54	5824.0
55	4270.0
56	4270.0
57	4223.5
58	4177.0
59	3902.0
60	3627.0
61	3627.0
62	3354.5
63	3082.0
64	2733.0
65	2384.0
66	2384.0
67	2126.5
68	1869.0
69	1869.0
70	1610.0
71	1351.0
72	1084.0
73	817.0
74	817.0
75	695.5
76	574.0
77	574.0
78	407.0
79	240.0
80	174.0
81	108.0
82	108.0
83	72.5
84	37.0
85	37.0
86	27.0
87	17.0
88	15.5
89	14.0
90	14.0
91	9.5
92	5.0
93	6.5
94	8.0
95	8.0
96	11.5
97	15.0
98	15.0
99	42.5
100	70.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11115986543805763
2	0.053824776948954225
3	0.011115986543805762
4	0.00292525961679099
5	5.85051923358198E-4
6	0.0
7	0.0
8	5.85051923358198E-4
9	0.001170103846716396
10	0.001170103846716396
11	0.002340207693432792
12	0.002340207693432792
13	0.01228609039052216
14	0.009360830773731168
15	0.02340207693432792
16	0.010530934620447564
17	0.019891765394178734
18	0.004680415386865584
19	0.007605675003656575
20	0.00585051923358198
21	0.004680415386865584
22	0.007020623080298377
23	0.007605675003656575
24	0.01521135000731315
25	0.017551557700745943
26	0.019306713470820535
27	0.009360830773731168
28	0.00585051923358198
29	0.01228609039052216
30	0.0035103115401491883
31	0.004095363463507386
32	0.006435571156940178
33	0.008775778850372971
34	0.013456194237238556
35	0.01521135000731315
36	0.01521135000731315
37	0.008190726927014773
38	0.0035103115401491883
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	170925.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.24981717127395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.65493867010976	28.1497732923797
2	12.304712717882504	8.920871727365803
3	4.062298256939961	4.417727073277753
4	1.8867010974822467	2.735702793622934
5	1.0555196901226598	1.9131197893813074
6	0.6132989025177534	1.3339183852566916
7	0.36636539703034215	0.9296475062161768
8	0.3066494512588767	0.889278923504461
9	0.2275661717236927	0.7424308907415533
>10	1.1927049709489994	8.390229632879919
>50	0.1275016139444803	3.351177416995758
>100	0.15978050355067785	11.881234459558286
>500	0.019367333763718526	5.311101360245721
>1k	0.020981278244028408	17.14553166593535
>5k	0.0016139444803098776	3.8882550826385844
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6646	3.8882550826385844	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4916	2.8761152552289015	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	4466	2.6128418897177124	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	3789	2.2167617376042124	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2616	1.530495831505046	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	2485	1.453854029545122	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2300	1.3456194237238555	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	1422	0.8319438350153576	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	1412	0.8260933157817757	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	1361	0.7962556676905075	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1262	0.7383355272780459	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	1147	0.6710545560918532	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	1081	0.632441129150212	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	1049	0.6137194676027498	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	964	0.5639900541173029	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	877	0.5130905367851397	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	861	0.5037297060114085	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	861	0.5037297060114085	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	802	0.46921164253327485	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	766	0.4481497732923797	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	754	0.4411291502120813	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	751	0.4393739944420067	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG	668	0.3908146848032763	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	656	0.3837940617229779	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	610	0.35688167324850084	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	508	0.2972063770659646	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC	498	0.29135585783238266	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	495	0.28960070206230804	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA	479	0.28023987128857686	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	471	0.27555945590171127	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	444	0.25976305397103994	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	436	0.25508263858417435	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA	415	0.24279654819365218	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA	397	0.2322656135732046	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	372	0.2176393154892497	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	365	0.21354395202574228	No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA	345	0.2018429135585783	No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	338	0.19774755009507095	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	335	0.19599239432499635	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	315	0.18429135585783238	No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCA	297	0.1737604212373848	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	289	0.16908000585051924	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	283	0.16556969431037005	No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA	282	0.16498464238701185	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT	277	0.16205938277022086	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	271	0.15854907123007167	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT	271	0.15854907123007167	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	268	0.15679391545999707	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	268	0.15679391545999707	No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTG	265	0.15503875968992248	No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA	263	0.15386865584320608	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	262	0.15328360391984788	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	254	0.1486031885329823	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT	250	0.1462629808395495	No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA	237	0.13865730583589295	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT	229	0.13397689044902736	No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	222	0.12988152698551997	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT	220	0.12871142313880357	No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTT	219	0.12812637121544537	No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA	212	0.124031007751938	No Hit
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCA	208	0.1216908000585052	No Hit
GTATCAACGCAGAGTACATGGGACGCAGAGTACTTTTT	208	0.1216908000585052	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTA	206	0.12052069621178879	No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA	206	0.12052069621178879	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	201	0.11759543659499781	No Hit
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	198	0.1158402808249232	No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAA	195	0.11408512505484862	No Hit
GGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA	193	0.11291502120813221	No Hit
GAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA	192	0.11232996928477403	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT	190	0.11115986543805763	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTA	188	0.10998976159134123	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG	183	0.10706450197455024	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAATC	181	0.10589439812783384	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT	177	0.10355419043440106	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	5.85051923358198E-4	0.0
16	0.0	0.0	0.0	0.0017551557700745941	0.0
17	0.0	0.0	0.0	0.002340207693432792	0.0
18	0.0	0.0	0.0	0.00292525961679099	0.0
19	0.0	0.0	0.0	0.004095363463507386	0.0
20	0.0	0.0	0.0	0.005265467310223783	0.0
21	0.0	0.0	0.0	0.00585051923358198	0.0
22	0.0	0.0	0.0	0.009945882697089367	0.0
23	0.0	0.0	0.0	0.025742284627760712	0.0
24	0.0	0.0	0.0	0.05089951733216323	0.0
25	0.0	0.0	0.0	0.05616498464238701	0.0
26	0.0	0.0	0.0	0.06611086733947638	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACC	20	0.003746834	31.996197	4
GCTCACC	20	0.003746834	31.996197	6
TACCAAT	35	0.0020608185	22.854425	20
CTTCAAT	50	2.7822083E-5	22.397339	28
TCCATGA	55	5.8014703E-5	20.361216	30
ATGCTTC	40	0.004471224	19.997623	25
GATCAAC	40	0.004471224	19.997623	21
CAATGTA	40	0.004471224	19.997623	31
GGATCAA	45	0.008826387	17.775667	20
AATGCTT	45	0.008826387	17.775667	24
TGGGATC	45	0.008826387	17.775667	18
ATACCAA	45	0.008826387	17.775667	19
CCATGAA	65	2.0891515E-4	17.22872	31
TGCTTCA	70	3.676775E-4	15.998098	26
TACATGC	60	0.0024324364	15.998098	31
AATGTAA	60	0.0024324364	15.998098	32
CATGAAC	75	6.211505E-4	14.931559	32
ACATGCA	75	6.211505E-4	14.931559	32
AAAAAGT	140	1.2405508E-8	14.868428	1
GCTTCAA	65	0.0041456143	14.767475	27
>>END_MODULE