FastQCFastQC Report
Thu 2 Feb 2017
SRR4062733_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062733_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170925
Sequences flagged as poor quality0
Sequence length38
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT81944.793915459997074No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT63113.692262688313588No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA47132.7573497147871873No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC42982.5145531665935352No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT36772.151235922188094No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29201.7083516162059382No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA21021.2297791428989322No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG14500.8483252888693872No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG13750.8044463946175223No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA11890.6956267368728974No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA11750.6874360099458827No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11750.6874360099458827No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11540.6751499195553605No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11540.6751499195553605No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA11130.6511627906976745No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT9890.5786163522012578No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA9630.5634050021939447No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC9480.5546292233435718No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG9470.5540441714202136No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC9450.5528740675734971No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA9130.5341524060260349No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC9100.5323972502559602No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG8610.5037297060114085No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA8120.47506216176685684No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG6190.3621471405587246No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT6160.36039198478865No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC5980.3498610501682024No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA5890.34459558285797864No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA5140.3007166886061138No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA5020.29369606552581545No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4980.29135585783238266No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4940.2890156501389498No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT4350.2544975866608162No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC4290.250987275120667No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA4170.2439666520403686No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT4140.24221149627029398No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA4060.23753108088342842No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT3890.22758519818633902No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3880.22700014626298085No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3850.22524499049290625No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA3820.22348983472283163No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG3810.22290478279947346No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA3690.21588415971917507No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT3580.2094485885622349No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTG3470.2030130174052947No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG3420.20008775778850374No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC3370.19716249817171272No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA3320.19423723855492175No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG3150.18429135585783238No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3010.1761006289308176No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2970.1737604212373848No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT2950.1725903173906684No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCA2890.16908000585051924No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA2840.16615474623372822No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA2810.16439959046365366No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA2760.16147433084686266No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTT2730.15971917507678807No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2700.15796401930671347No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT2680.15679391545999707No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT2680.15679391545999707No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA2610.15269855199648968No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG2570.1503583443030569No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA2510.14684803276290773No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT2500.1462629808395495No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG2440.14275266929940034No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT2350.13748720198917655No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAA2320.13573204621910193No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA2220.12988152698551997No Hit
GGTATCAACGCAGAGTACATGGGTGGTATCAACGCAAA2220.12988152698551997No Hit
GTATCAACGCAGAGTACATGGGACGCAGAGTACTTTTT2050.11993564428843059No Hit
GATACCACTGCTTCCATGTACTCTGCGTTGATACCACT2020.118180488518356No Hit
GCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC1990.11642533274828142No Hit
ACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAG1990.11642533274828142No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTA1960.11467017697820682No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1880.10998976159134123No Hit
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA1870.10940470966798303No Hit
TGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG1810.10589439812783384No Hit
GTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC1810.10589439812783384No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTG1790.10472429428111744No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT1780.10413924235775925No Hit
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCA1750.10238408658768466No Hit
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC1740.10179903466432645No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGTGG200.00374465432.06
ATACATG800.032.01
TGCTTAC550.001358215117.45454626
AAAAGTA2000.015.22
CTGCTTA756.2059076E-414.93333325
AAAAAGT2100.014.4761911
AAGTACT2250.014.2222224
AGTACTC2450.013.7142865
GGCAGAG700.006772639713.71428521
AAAGTAC2400.013.3333333
TTTTGGT1801.7389539E-913.33333332
TGGGTGG4600.013.2173916
GGGCAGA850.001598566413.17647120
CATGGGT6850.013.0802924
ATGGGTG5900.012.7457625
TGGGGTG6800.012.06
GGGGTGG6800.011.7647067
ATGGAAG15800.011.7468365
TGGAAGC15800.011.7468366
CATGGAA16350.011.645264
Produced by FastQC (version 0.11.5)