##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062733_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	170925
Sequences flagged as poor quality	0
Sequence length	38
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.826450197455024	32.0	32.0	32.0	14.0	32.0
2	31.219154599970746	32.0	32.0	32.0	32.0	32.0
3	31.341407049875677	32.0	32.0	32.0	32.0	32.0
4	31.49524060260348	32.0	32.0	32.0	32.0	32.0
5	31.280930232558138	32.0	32.0	32.0	32.0	32.0
6	34.66998391107211	36.0	36.0	36.0	32.0	36.0
7	34.787497440397836	36.0	36.0	36.0	32.0	36.0
8	34.84167909902004	36.0	36.0	36.0	32.0	36.0
9	34.80114085125055	36.0	36.0	36.0	32.0	36.0
10	34.681614743308465	36.0	36.0	36.0	32.0	36.0
11	34.867333625859295	36.0	36.0	36.0	32.0	36.0
12	34.78300424162644	36.0	36.0	36.0	32.0	36.0
13	34.760819072692705	36.0	36.0	36.0	32.0	36.0
14	34.65202281702501	36.0	36.0	36.0	32.0	36.0
15	34.68183121252011	36.0	36.0	36.0	32.0	36.0
16	34.61786748573936	36.0	36.0	36.0	32.0	36.0
17	34.57186485300571	36.0	36.0	36.0	32.0	36.0
18	34.48342840427088	36.0	36.0	36.0	32.0	36.0
19	34.440204768173174	36.0	36.0	36.0	32.0	36.0
20	34.326195699868364	36.0	36.0	36.0	32.0	36.0
21	34.21854029545122	36.0	36.0	36.0	32.0	36.0
22	34.23639022963288	36.0	36.0	36.0	32.0	36.0
23	34.13823606845107	36.0	36.0	36.0	32.0	36.0
24	34.150516308322366	36.0	36.0	36.0	32.0	36.0
25	34.11318999561211	36.0	36.0	36.0	32.0	36.0
26	33.978628053239724	36.0	36.0	36.0	32.0	36.0
27	33.937931841450926	36.0	36.0	36.0	32.0	36.0
28	33.82956267368729	36.0	36.0	36.0	32.0	36.0
29	33.72701477256106	36.0	36.0	36.0	32.0	36.0
30	33.67020623080298	36.0	36.0	36.0	27.0	36.0
31	33.506140119935644	36.0	36.0	36.0	21.0	36.0
32	33.351739066842185	36.0	36.0	36.0	21.0	36.0
33	33.07304958315051	36.0	36.0	36.0	14.0	36.0
34	32.854134854468334	36.0	36.0	36.0	14.0	36.0
35	32.538440836624254	36.0	36.0	36.0	14.0	36.0
36	32.39871288576861	36.0	36.0	36.0	14.0	36.0
37	32.22151235922188	36.0	36.0	36.0	14.0	36.0
38	31.776047974257715	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	1.0
20	7.0
21	13.0
22	21.0
23	76.0
24	238.0
25	472.0
26	1147.0
27	2269.0
28	3964.0
29	6250.0
30	8752.0
31	11845.0
32	15371.0
33	21195.0
34	37336.0
35	61966.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.629474472541744	17.29583299203108	16.092303731701325	14.982388803725852
2	11.465262542050606	25.616498464238703	39.68173175369314	23.236507240017552
3	18.705865145531668	38.39286236653503	26.80181366096241	16.099458826970896
4	10.161766856808542	19.161035541904344	31.057481351469946	39.61971624981717
5	9.201750485595937	43.152512227656736	28.998853291521375	18.64688399522595
6	25.68962995465848	34.66315635512652	21.53634635073863	18.11086733947638
7	22.65321047242943	35.89293549802545	22.894836916776367	18.559017112768757
8	33.73448862938282	25.358202231414147	20.973186756609703	19.93412238259333
9	30.652937046571495	14.142873859115376	20.300140416569153	34.90404867774398
10	18.463977720831725	29.790781760101098	28.43753290974831	23.30770760931887
11	30.663288887848772	26.260085068540434	19.87058348593795	23.20604255767284
12	25.666227877724147	29.761006289308177	29.2988152698552	15.273950563112477
13	34.58534444931988	23.900541173029104	27.335381015065085	14.17873336258593
14	28.150943396226413	19.316074301594266	32.30539710399298	20.22758519818634
15	25.484276729559745	22.89191165715957	37.773877431622054	13.849934181658622
16	18.599385695480475	27.05923650724002	33.878016673979815	20.46336112329969
17	17.401199356442884	24.843059821559162	34.99546584759398	22.76027497440398
18	19.907561796109405	20.579786456047973	42.130759104870556	17.381892642972062
19	18.293988591487494	28.10706450197455	40.187801667397984	13.411145239139973
20	22.573643410852714	17.024425917800205	45.371361708351614	15.030568962995467
21	24.204183121252008	22.216176685680857	35.23007166886061	18.34956852420652
22	20.699722100336405	23.750767880649406	36.57159572912096	18.977914289893228
23	17.669738189264297	24.970016088927892	37.414070498756764	19.946175223051046
24	16.168494953927162	28.60494368875238	38.11788796255668	17.108673394763784
25	21.43220710838087	22.673102237823606	36.33113938862074	19.563551265174784
26	16.59441275413193	23.526692994003216	39.680561649846425	20.19833260201843
27	19.8964458095656	26.82697089366681	39.26575983618546	14.010823460582127
28	17.885037297060112	23.108380868802104	40.125786163522015	18.880795670615765
29	15.055726195699867	27.145239139973672	42.901857539856664	14.897177124469795
30	19.24294281117449	24.750621617668568	41.02267076203013	14.983764809126809
31	14.580664033933012	27.006581834137776	38.87845546292233	19.534298669006876
32	13.983911072107649	28.943103700453417	38.989615328360394	18.083369899078544
33	14.659060991663008	28.360391984788652	37.78557847008922	19.19496855345912
34	13.873921310516307	29.542781921895568	39.907561796109405	16.67573497147872
35	18.0125786163522	28.60786894836917	37.96752961825362	15.412022817025012
36	17.930086295158695	30.707035249378382	36.750621617668564	14.612256837794355
37	18.35366388767003	31.608600263273367	36.70732777534006	13.330408073716542
38	16.334064648237533	32.25391253473745	34.131929208717274	17.280093608307737
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	745.0
1	596.0
2	447.0
3	447.0
4	1401.5
5	2356.0
6	2356.0
7	2678.5
8	3001.0
9	3010.0
10	3019.0
11	3019.0
12	3570.0
13	4121.0
14	4691.0
15	5261.0
16	5261.0
17	7499.0
18	9737.0
19	9737.0
20	9871.5
21	10006.0
22	7700.5
23	5395.0
24	5395.0
25	3859.0
26	2323.0
27	2323.0
28	3151.5
29	3980.0
30	4961.0
31	5942.0
32	5942.0
33	4774.5
34	3607.0
35	3607.0
36	3476.5
37	3346.0
38	3924.0
39	4502.0
40	4502.0
41	6016.5
42	7531.0
43	9754.0
44	11977.0
45	11977.0
46	21913.5
47	31850.0
48	31850.0
49	26420.0
50	20990.0
51	14596.5
52	8203.0
53	8203.0
54	6227.5
55	4252.0
56	4252.0
57	4276.0
58	4300.0
59	3914.0
60	3528.0
61	3528.0
62	3268.0
63	3008.0
64	2714.0
65	2420.0
66	2420.0
67	2171.5
68	1923.0
69	1923.0
70	1628.5
71	1334.0
72	1104.0
73	874.0
74	874.0
75	709.5
76	545.0
77	545.0
78	394.0
79	243.0
80	162.0
81	81.0
82	81.0
83	55.0
84	29.0
85	29.0
86	18.0
87	7.0
88	7.0
89	7.0
90	7.0
91	5.5
92	4.0
93	6.5
94	9.0
95	9.0
96	13.5
97	18.0
98	18.0
99	11.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006435571156940178
2	0.0
3	0.0
4	0.0
5	5.85051923358198E-4
6	0.0
7	0.0
8	0.001170103846716396
9	0.00292525961679099
10	0.0017551557700745941
11	0.002340207693432792
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	170925.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.672370922919406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.51536870104921	23.917507678806494
2	13.368183833308702	8.46804153868656
3	4.521944731786612	4.296621325142606
4	2.1002659967489286	2.660816147433085
5	1.2099157676961725	1.9160450489980985
6	0.6779222698389242	1.2882843352347522
7	0.41561992020097527	0.9214567792891618
8	0.3103295404167282	0.7863097849934182
9	0.2308999556672085	0.6581834137779727
>10	1.2505541598936014	7.574082199795232
>50	0.16070636914437714	3.636097703671201
>100	0.18287276488842913	12.444054409828873
>500	0.02770799468006502	6.860318853298231
>1k	0.024013595389389686	16.086002632733656
>5k	0.003694399290675336	8.486178148310662
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	8194	4.793915459997074	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	6311	3.692262688313588	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	4713	2.7573497147871873	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	4298	2.5145531665935352	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	3677	2.151235922188094	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2920	1.7083516162059382	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	2102	1.2297791428989322	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	1450	0.8483252888693872	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	1375	0.8044463946175223	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	1189	0.6956267368728974	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	1175	0.6874360099458827	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1175	0.6874360099458827	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1154	0.6751499195553605	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1154	0.6751499195553605	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	1113	0.6511627906976745	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	989	0.5786163522012578	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	963	0.5634050021939447	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	948	0.5546292233435718	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	947	0.5540441714202136	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	945	0.5528740675734971	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	913	0.5341524060260349	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	910	0.5323972502559602	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG	861	0.5037297060114085	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	812	0.47506216176685684	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	619	0.3621471405587246	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	616	0.36039198478865	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC	598	0.3498610501682024	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	589	0.34459558285797864	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA	514	0.3007166886061138	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	502	0.29369606552581545	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	498	0.29135585783238266	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	494	0.2890156501389498	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	435	0.2544975866608162	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	429	0.250987275120667	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA	417	0.2439666520403686	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	414	0.24221149627029398	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	406	0.23753108088342842	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT	389	0.22758519818633902	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	388	0.22700014626298085	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	385	0.22524499049290625	No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	382	0.22348983472283163	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	381	0.22290478279947346	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA	369	0.21588415971917507	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT	358	0.2094485885622349	No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTG	347	0.2030130174052947	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	342	0.20008775778850374	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	337	0.19716249817171272	No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA	332	0.19423723855492175	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	315	0.18429135585783238	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	301	0.1761006289308176	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	297	0.1737604212373848	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT	295	0.1725903173906684	No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCA	289	0.16908000585051924	No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA	284	0.16615474623372822	No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA	281	0.16439959046365366	No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA	276	0.16147433084686266	No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTT	273	0.15971917507678807	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	270	0.15796401930671347	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT	268	0.15679391545999707	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT	268	0.15679391545999707	No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA	261	0.15269855199648968	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG	257	0.1503583443030569	No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA	251	0.14684803276290773	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT	250	0.1462629808395495	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG	244	0.14275266929940034	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT	235	0.13748720198917655	No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAA	232	0.13573204621910193	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	222	0.12988152698551997	No Hit
GGTATCAACGCAGAGTACATGGGTGGTATCAACGCAAA	222	0.12988152698551997	No Hit
GTATCAACGCAGAGTACATGGGACGCAGAGTACTTTTT	205	0.11993564428843059	No Hit
GATACCACTGCTTCCATGTACTCTGCGTTGATACCACT	202	0.118180488518356	No Hit
GCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC	199	0.11642533274828142	No Hit
ACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAG	199	0.11642533274828142	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTA	196	0.11467017697820682	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	188	0.10998976159134123	No Hit
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	187	0.10940470966798303	No Hit
TGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	181	0.10589439812783384	No Hit
GTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC	181	0.10589439812783384	No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTG	179	0.10472429428111744	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT	178	0.10413924235775925	No Hit
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCA	175	0.10238408658768466	No Hit
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	174	0.10179903466432645	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	5.85051923358198E-4	0.0	0.0	5.85051923358198E-4	0.0
16	5.85051923358198E-4	0.0	0.0	0.0017551557700745941	0.0
17	5.85051923358198E-4	0.0	0.0	0.002340207693432792	0.0
18	5.85051923358198E-4	0.0	0.0	0.00292525961679099	0.0
19	5.85051923358198E-4	0.0	0.0	0.004095363463507386	0.0
20	5.85051923358198E-4	0.0	0.0	0.005265467310223783	0.0
21	5.85051923358198E-4	0.0	0.0	0.006435571156940178	0.0
22	5.85051923358198E-4	0.0	0.0	0.010530934620447565	0.0
23	5.85051923358198E-4	0.0	0.0	0.02632733655111891	0.0
24	5.85051923358198E-4	0.0	0.0	0.049729413485446836	0.0
25	5.85051923358198E-4	0.0	0.0	0.054994880795670616	0.0
26	5.85051923358198E-4	0.0	0.0	0.06611086733947638	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGGTGG	20	0.003744654	32.0	6
ATACATG	80	0.0	32.0	1
TGCTTAC	55	0.0013582151	17.454546	26
AAAAGTA	200	0.0	15.2	2
CTGCTTA	75	6.2059076E-4	14.933333	25
AAAAAGT	210	0.0	14.476191	1
AAGTACT	225	0.0	14.222222	4
AGTACTC	245	0.0	13.714286	5
GGCAGAG	70	0.0067726397	13.714285	21
AAAGTAC	240	0.0	13.333333	3
TTTTGGT	180	1.7389539E-9	13.333333	32
TGGGTGG	460	0.0	13.217391	6
GGGCAGA	85	0.0015985664	13.176471	20
CATGGGT	685	0.0	13.080292	4
ATGGGTG	590	0.0	12.745762	5
TGGGGTG	680	0.0	12.0	6
GGGGTGG	680	0.0	11.764706	7
ATGGAAG	1580	0.0	11.746836	5
TGGAAGC	1580	0.0	11.746836	6
CATGGAA	1635	0.0	11.64526	4
>>END_MODULE