FastQCFastQC Report
Thu 2 Feb 2017
SRR4062732_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062732_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7739808
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT79330.10249608259016245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA72150.021.2687151
GGTATCA25100.019.7195431
TCTAGCG12700.015.8728128
CTAGCGG12550.015.80817829
TAGCGGC13300.015.27762830
TATCAAC105650.014.3625242
TCAACGC105050.014.2526854
CAACGCA107600.013.8853595
GTATTAG18050.013.8439311
AACGCAG109700.013.6926366
ACCGTCG11050.013.6092118
ATCAACG110150.013.6091463
TACCGTC12050.013.27647
ATCGTTT10650.012.76947929
ATACCGT12650.012.6455456
GTCCTAC19600.012.5039561
GTCCTAT20000.012.4941491
CGTCGTA12750.012.4236310
AACCGCG8850.012.2922957
CGCCGGT16350.012.2309337
Produced by FastQC (version 0.11.5)