##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062732_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7739808
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.284462999598958	32.0	32.0	32.0	32.0	32.0
2	30.912641760622485	32.0	32.0	32.0	32.0	32.0
3	30.95495728059404	32.0	32.0	32.0	32.0	32.0
4	31.025643659377597	32.0	32.0	32.0	32.0	32.0
5	30.93735115909852	32.0	32.0	32.0	32.0	32.0
6	34.59428153773324	36.0	36.0	36.0	32.0	36.0
7	34.544950856662076	36.0	36.0	36.0	32.0	36.0
8	34.53385768742584	36.0	36.0	36.0	32.0	36.0
9	34.66911155935651	36.0	36.0	36.0	32.0	36.0
10	34.40017736874093	36.0	36.0	36.0	32.0	36.0
11	34.639778144367405	36.0	36.0	36.0	32.0	36.0
12	34.476740379089506	36.0	36.0	36.0	32.0	36.0
13	34.548338020788115	36.0	36.0	36.0	32.0	36.0
14	34.456191290533305	36.0	36.0	36.0	32.0	36.0
15	34.40508924769193	36.0	36.0	36.0	32.0	36.0
16	34.42290558112036	36.0	36.0	36.0	32.0	36.0
17	34.36202758518041	36.0	36.0	36.0	32.0	36.0
18	34.38195417767469	36.0	36.0	36.0	32.0	36.0
19	34.37110959341627	36.0	36.0	36.0	32.0	36.0
20	34.357378115839566	36.0	36.0	36.0	32.0	36.0
21	34.35056076326441	36.0	36.0	36.0	32.0	36.0
22	34.32586002133386	36.0	36.0	36.0	32.0	36.0
23	34.27218633847248	36.0	36.0	36.0	32.0	36.0
24	34.252652003770635	36.0	36.0	36.0	32.0	36.0
25	34.23172952093902	36.0	36.0	36.0	32.0	36.0
26	34.1957409020999	36.0	36.0	36.0	32.0	36.0
27	34.19285491319681	36.0	36.0	36.0	32.0	36.0
28	34.175873484200125	36.0	36.0	36.0	32.0	36.0
29	34.14159640652585	36.0	36.0	36.0	32.0	36.0
30	34.121644748810304	36.0	36.0	36.0	32.0	36.0
31	34.137378989246244	36.0	36.0	36.0	32.0	36.0
32	34.10078402461663	36.0	36.0	36.0	32.0	36.0
33	34.07789818558807	36.0	36.0	36.0	32.0	36.0
34	34.08246638676308	36.0	36.0	36.0	32.0	36.0
35	34.058456488843134	36.0	36.0	36.0	32.0	36.0
36	34.01853469750154	36.0	36.0	36.0	32.0	36.0
37	34.014554107802155	36.0	36.0	36.0	32.0	36.0
38	33.45071221404975	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	2.0
7	16.0
8	13.0
9	17.0
10	65.0
11	103.0
12	31.0
13	38.0
14	485.0
15	1333.0
16	1913.0
17	2332.0
18	2966.0
19	3757.0
20	5303.0
21	8071.0
22	12313.0
23	19537.0
24	30700.0
25	46861.0
26	70158.0
27	101434.0
28	142991.0
29	192822.0
30	260337.0
31	351742.0
32	480284.0
33	724783.0
34	1634201.0
35	3645199.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.98704889261337	17.226609257399556	11.56019092324197	26.226150926745103
2	16.588686188050573	19.338339958862296	38.208163601419585	25.864810251667542
3	19.05388673425111	23.446354257253844	28.435847544509773	29.063911463985274
4	12.76660210241464	15.553085136306544	36.19726246020939	35.483050301069426
5	14.64646249308928	36.31111553845086	33.93537832501075	15.107043643449103
6	34.039244779664955	35.35152997223048	17.209582545035833	13.399642703068732
7	30.45025934758571	30.305307394009617	20.890161846433877	18.354271411970792
8	27.714706065896717	33.43745344666818	19.433875308325987	19.41396517910912
9	27.192314003351512	15.053457522749325	18.621577588022415	39.13265088587675
10	15.57987955974408	26.705531726972282	32.014487250333374	25.700101462950258
11	36.82223286296705	21.374619136130583	22.87453347325367	18.928614527648698
12	24.99870471358924	23.398286294086738	28.89859567696061	22.704413315363414
13	28.95366759843529	19.837225638247723	25.409785224575536	25.79932153874145
14	23.336922480747365	19.900321369321976	25.29466392025606	31.468092229674593
15	24.91301315903743	27.40076443999653	22.252399601341544	25.4338227996245
16	25.688028109319532	26.003123430015112	23.676974381981587	24.631874078683765
17	24.029448785114845	26.072875017122886	25.25398737170756	24.643688826054706
18	24.655886384653442	25.240507084743385	26.094503472307913	24.00910305829526
19	25.456427953795906	25.16391815824524	25.369998144517776	24.009655743441076
20	25.490961709838462	24.92869234125704	25.010507987039222	24.56983796186528
21	26.146695046468633	24.605148755566873	24.736504185710682	24.51165201225381
22	25.460062572205285	24.76419132214762	25.032448364621807	24.743297741025287
23	24.327814522899267	24.636586391063396	25.53203256163194	25.503566524405397
24	24.731123743165835	25.167373289277645	25.228313836062693	24.87318913149383
25	24.834092331437745	24.783423538258297	25.398246247233473	24.984237883070485
26	24.587123929308344	25.398163611079845	25.65567365296351	24.3590388066483
27	25.076807957300502	25.234862368487732	24.770312647182678	24.918017027029087
28	24.484740653452384	25.05229440954705	25.460287065265774	25.00267787173479
29	24.664034705398098	25.187605739214096	25.300734503523657	24.847625051864146
30	24.612484630917194	25.204352596606217	25.56821030228576	24.614952470190826
31	25.064953691261294	24.946597651485394	24.880403547580162	25.10804510967315
32	24.672595470456613	25.154358694136754	24.831641293717414	25.341404541689222
33	24.398241875470656	24.927912035886084	25.322102306763167	25.351743781880092
34	25.007727244299282	24.91369520957372	25.49361326460307	24.584964281523927
35	25.398837792159174	24.903744850292956	25.352756790131277	24.344660567416597
36	24.60474310854184	25.125273982320923	25.17506310541984	25.094919803717396
37	25.39433421927245	24.966568924353442	24.88624906109362	24.752847795280484
38	24.603155221729544	25.097860146335076	25.28840559317494	25.010579038760433
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	81.0
1	197.0
2	313.0
3	313.0
4	851.0
5	1389.0
6	1389.0
7	1604.5
8	1820.0
9	1830.0
10	1840.0
11	1840.0
12	2644.0
13	3448.0
14	5648.0
15	7848.0
16	7848.0
17	12954.5
18	18061.0
19	18061.0
20	25295.0
21	32529.0
22	42591.0
23	52653.0
24	52653.0
25	68706.5
26	84760.0
27	84760.0
28	109548.0
29	134336.0
30	164126.5
31	193917.0
32	193917.0
33	241318.5
34	288720.0
35	288720.0
36	323646.5
37	358573.0
38	403344.5
39	448116.0
40	448116.0
41	480548.5
42	512981.0
43	563910.5
44	614840.0
45	614840.0
46	632436.0
47	650032.0
48	650032.0
49	686809.5
50	723587.0
51	730132.0
52	736677.0
53	736677.0
54	709870.0
55	683063.0
56	683063.0
57	649493.5
58	615924.0
59	554010.0
60	492096.0
61	492096.0
62	448341.5
63	404587.0
64	336274.5
65	267962.0
66	267962.0
67	220073.5
68	172185.0
69	172185.0
70	135287.5
71	98390.0
72	75957.5
73	53525.0
74	53525.0
75	40055.5
76	26586.0
77	26586.0
78	24061.0
79	21536.0
80	17031.5
81	12527.0
82	12527.0
83	12102.5
84	11678.0
85	11678.0
86	7797.0
87	3916.0
88	3283.5
89	2651.0
90	2651.0
91	2026.5
92	1402.0
93	1234.5
94	1067.0
95	1067.0
96	952.0
97	837.0
98	837.0
99	2096.0
100	3355.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12378860044073443
2	0.05587993914060917
3	0.012222525416651163
4	0.002984570159879935
5	2.71324559989085E-4
6	3.4884586284310927E-4
7	2.584043428467476E-5
8	3.7468629712778407E-4
9	2.3256390856207286E-4
10	4.780480342664831E-4
11	0.002144756045628005
12	0.002674484948463838
13	0.014470643199417866
14	0.00952220003390265
15	0.02397992301617818
16	0.011369791085256896
17	0.021034113507725258
18	0.005568613588347411
19	0.007493725942555681
20	0.005904539234048183
21	0.006473028788311028
22	0.007610007896836717
23	0.009832285245318746
24	0.014444802765133193
25	0.017015925976458332
26	0.018979798982093614
27	0.009057072216778504
28	0.005852858365478834
29	0.010840062182421064
30	0.003152532982730321
31	0.005788257279767147
32	0.006834794868296475
33	0.008423981576803973
34	0.012351727588074535
35	0.01629239381648744
36	0.015116654056534735
37	0.009703083073895372
38	0.005775337062624809
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	7739808.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.42706788758563
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.23408790397035	40.96396865519611
2	10.200284344227276	10.287408621980514
3	3.1638241683393145	4.786271283636923
4	1.5234850702477596	3.0729954025242776
5	0.8685214971853275	2.189849625019601
6	0.5447281892321557	1.6481427227214902
7	0.3938202859769332	1.3901441607527039
8	0.2763854747211146	1.114984727752338
9	0.20940684489291844	0.9503795865186281
>10	1.3929621735397104	13.243223644126534
>50	0.1109599517816884	3.864234450793036
>100	0.06584203226787641	6.509127090422802
>500	0.00866947063077567	3.070425234618915
>1k	0.006816802717888481	6.312235103816888
>5k	2.0579026903576436E-4	0.5966096901192439
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	7933	0.10249608259016245	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.421223885657112E-4
2	0.0	0.0	0.0	0.0	1.421223885657112E-4
3	0.0	0.0	0.0	0.0	1.421223885657112E-4
4	0.0	0.0	0.0	0.0	1.421223885657112E-4
5	0.0	1.2920217142337382E-5	0.0	0.0	1.421223885657112E-4
6	0.0	1.2920217142337382E-5	0.0	0.0	1.938032571350607E-4
7	0.0	1.2920217142337382E-5	0.0	0.0	1.938032571350607E-4
8	0.0	1.2920217142337382E-5	0.0	0.0	1.938032571350607E-4
9	0.0	1.2920217142337382E-5	0.0	2.5840434284674764E-5	1.938032571350607E-4
10	0.0	1.2920217142337382E-5	0.0	6.460108571168691E-5	1.938032571350607E-4
11	0.0	1.2920217142337382E-5	0.0	9.044151999636167E-5	2.067234742773981E-4
12	0.0	1.2920217142337382E-5	0.0	9.044151999636167E-5	4.2636716569713356E-4
13	0.0	1.2920217142337382E-5	0.0	9.044151999636167E-5	5.038884685511578E-4
14	1.2920217142337382E-5	1.2920217142337382E-5	0.0	9.044151999636167E-5	5.168086856934953E-4
15	1.2920217142337382E-5	2.5840434284674764E-5	0.0	1.6796282285038596E-4	5.814097714051821E-4
16	1.2920217142337382E-5	2.5840434284674764E-5	0.0	1.8088303999272334E-4	5.814097714051821E-4
17	1.2920217142337382E-5	2.5840434284674764E-5	0.0	1.8088303999272334E-4	5.943299885475196E-4
18	1.2920217142337382E-5	2.5840434284674764E-5	0.0	2.71324559989085E-4	6.201704228321942E-4
19	1.2920217142337382E-5	2.5840434284674764E-5	0.0	3.230054285584345E-4	6.201704228321942E-4
20	1.2920217142337382E-5	2.5840434284674764E-5	0.0	3.7468629712778407E-4	6.847715085438812E-4
21	1.2920217142337382E-5	2.5840434284674764E-5	0.0	4.651278171241457E-4	6.976917256862185E-4
22	1.2920217142337382E-5	2.5840434284674764E-5	0.0	5.814097714051821E-4	7.364523771132307E-4
23	1.2920217142337382E-5	2.5840434284674764E-5	0.0	6.976917256862185E-4	7.493725942555681E-4
24	1.2920217142337382E-5	2.5840434284674764E-5	0.0	9.560960685329662E-4	7.622928113979055E-4
25	1.2920217142337382E-5	2.5840434284674764E-5	0.0	0.0012403408456643885	7.622928113979055E-4
26	1.2920217142337382E-5	2.5840434284674764E-5	0.0	0.0015762664913651606	7.622928113979055E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7215	0.0	21.268715	1
GGTATCA	2510	0.0	19.719543	1
TCTAGCG	1270	0.0	15.87281	28
CTAGCGG	1255	0.0	15.808178	29
TAGCGGC	1330	0.0	15.277628	30
TATCAAC	10565	0.0	14.362524	2
TCAACGC	10505	0.0	14.252685	4
CAACGCA	10760	0.0	13.885359	5
GTATTAG	1805	0.0	13.843931	1
AACGCAG	10970	0.0	13.692636	6
ACCGTCG	1105	0.0	13.609211	8
ATCAACG	11015	0.0	13.609146	3
TACCGTC	1205	0.0	13.2764	7
ATCGTTT	1065	0.0	12.769479	29
ATACCGT	1265	0.0	12.645545	6
GTCCTAC	1960	0.0	12.503956	1
GTCCTAT	2000	0.0	12.494149	1
CGTCGTA	1275	0.0	12.42363	10
AACCGCG	885	0.0	12.292295	7
CGCCGGT	1635	0.0	12.230933	7
>>END_MODULE